Proteolytic cleaning of a surface-bound rubisco protein stain

Onaizi, Sagheer A.; He, Lizhong; Middelberg, Anton P. J.

doi:10.1016/j.ces.2009.05.027

Cited by 39 publications

(14 citation statements)

References 52 publications

(60 reference statements)

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“…1, and their contact angles with Milli-Q water (MQW) is shown in Table 1. Before the assembly process took place, the SPR chips were thoroughly cleaned as described elsewhere [11][12][13][14]. Briefly, the chips were rinsed with HPLC grade ethanol and then with MQW several times before incubating in detergent (10 % (v/v) decon 90 aqueous solution) while slowly heating up the solution and the immersed chips until the temperature reached 70°C, and then the detergent solution was discarded and the chips were rinsed several times with MQW, HPLC grade ethanol and then with MQW again.…”

Section: Methodsmentioning

confidence: 99%

Adsorption of an anionic surfactant at air-liquid and different solid-liquid interfaces from solutions containing high counter-ion concentration

2015

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The self-assembly (adsorption) of the anionic surfactant, sodium dodecylbenzenesulphonate (SDBS), at airliquid and different (octadecanethiol, β-mercaptoethanol, α-lipoic acid) solid-liquid interfaces from aqueous solutions containing high concentrations of counter-ion has been investigated. SDBS adsorption at the solid-liquid interfaces was obtained using surface plasmon resonance (SPR) while its adsorption at the air-liquid interface was extracted from surface tension measurements. The results have demonstrated that SDBS packing at the air-liquid interface is similar to its packing at the hydrophobic octadecanethiol-liquid interface. Additionally, SDBS packing at the three solid-liquid interfaces increases with increasing surface hydrophobicity, irrespective whether the surface is neutral or negatively charged. Nonetheless, SDBS adsorption is always within monolayer coverage (no evidence of bilayer or admicelle formation). The results have also revealed that SDBS affinity for the solid-liquid interfaces increases with increasing surface hydrophobicity. Furthermore, SDBS affinity for the air-liquid interface is more than 10-fold its affinity for the solid-liquid interfaces.

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Section: Methodsmentioning

confidence: 99%

Adsorption of an anionic surfactant at air-liquid and different solid-liquid interfaces from solutions containing high counter-ion concentration

2015

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“…The mixed SAM solution was then added to the jar containing cleaned [9] gold slides. The self-assembly reaction was allowed to proceed for at least 1 d at room temperature.…”

Section: Design Of a Surface Plasmon Resonance (Spr) Biosensor Chip Wmentioning

confidence: 99%

“…This objective was achieved by a combination of phenylmethanesulfonyl fluoride (PMSF) treatment of SA and glutaraldehyde (GA) cross-linking of rubisco [9]. Measurements of the adsorption isotherm of partially deactivated SA onto cross-linked rubisco were conducted as follows.…”

Section: Adsorption Isotherm Of Sa Onto Immobilised Rubiscomentioning

confidence: 99%

“…Concurrently, the enzyme can be deactivated both in bulk and on the surface. With a series of simplifying assumptions [9], the following model equations have been shown to adequately represent the coupled transfer-reaction processes:…”

Section: Introductionmentioning

confidence: 99%

“…To achieve a decoupling of the rates of transfer and reaction we use a recently-published mathematical model [9]. The model included enzyme activity loss, which is an important issue in many enzymatic applications, especially for proteases, mainly due to autolysis [3,10,11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The construction, fouling and enzymatic cleaning of a textile dye surface

Onaizi

Middelberg

2010

Journal of Colloid and Interface Science

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Lysozyme binding to tethered bilayer lipid membranes prepared by rapid solvent exchange and vesicle fusion methods

2014

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Tethered bilayer lipid membranes (tBLMs) are important tools for studying protein-lipid interactions. The widely used methodology for the preparation of these membranes is the fusion of phospholipid vesicles from an aqueous medium onto an anchored phospholipid layer. The preparation of phospholipid vesicles is a long and tedious procedure. There is another simple method, rapid solvent exchange, for preparing lipid membranes. However, there is a lack of information on the effects of the preparation method of tBLMs on their interactions with proteins. Therefore, we present in this paper a comparative study on the binding of lysozyme onto tBLMs prepared by the abovementioned methods. The prepared tBLMs have either zwitterionic or anionic characteristics. The results show that lysozyme binding onto the prepared tBLMs is unaffected by the preparation method of the tBLMs, suggesting that the tedious fusion method might be replaced by the simple rapid solvent exchange method without altering the level of protein-lipid interactions.

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Proteolytic cleaning of a surface-bound rubisco protein stain

Cited by 39 publications

References 52 publications

Adsorption of an anionic surfactant at air-liquid and different solid-liquid interfaces from solutions containing high counter-ion concentration

Adsorption of an anionic surfactant at air-liquid and different solid-liquid interfaces from solutions containing high counter-ion concentration

The construction, fouling and enzymatic cleaning of a textile dye surface

Lysozyme binding to tethered bilayer lipid membranes prepared by rapid solvent exchange and vesicle fusion methods

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