Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae

Abstract

BackgroundThe predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown.FindingsThus, the objective of this work was to evaluate the production of proteases from nematophagous fungus Arthrobotrys sinensis in liquid medium and its nematicidal activity on first stage larvae of A. vasorum. Proteases were obtained in its crude form, using Whatman no.1 filter paper, followed by centrifugation for 5 min at 10 × g and 4°C. A zymogram was performed with co-polymerized casein in an acrylamide gel as substrate. An in vitro assay to evaluate the nematicidal action of the proteases of A. sinensis (SF53) produced in liquid medium on A. vasorum L1 was conducted. By the analysis of the zymogram, it was observed a single halo at the beginning of digestion of the gel, suggesting that the three proteases of SF53 are produced in an enzymatic complex of large molecular weight. Regarding nematicidal activity, within 24 hours, the proteases produced in liquid medium of A. sinensis (SF53) showed a percentage reduction of 64% on the number of L1 of A. vasorum.ConclusionIn the present work, it is suggested that the three proteases of SF53 are produced in an enzymatic complex and was also demonstrated that these enzymes were effective in destroying A. vasorum L1.

Antiparasitic drugs: in vitro tests against nematophagous fungi

“…The nematophagous fungi are the most studied microorganisms for this kind of purpose (Graminha et al, 2005;Soares et al, 2014). These fungi live in the soil organic matter, in which they developed a parasitic or predatory relation with nematodes, classified as: Ovicidal, endo-parasites and predators.…”

Interacción biológica de las enzimas producidas por hongos nematófagos saprófitos de suelo, contra huevos de Toxocara canis

“…La contaminación de suelos con los estadios infectantes, huevos y larvas, han demostrado ser la mayor fuente de contaminación con geohelmintos 57 . Además, el uso de extractos crudos enzimáticos es también un artificio, aunque reciente, que puede ser usado para combatir los huevos o larvas de geohelmintos 212 . lunata y T. ajelloi, para realizar los ensayos de interacción con huevos de T. canis, obedeciendo esa elección a: frecuencia de recuperación en los suelos, antecedentes de antagonismo con huevos de nematodos tanto positivo como negativo y actividad quitinolítica.…”