Proteins responding to drought and high-temperature stress in Populus × euramericana cv. ‘74/76’

Abstract

Proteomic analysis provides a powerful method of studying plant responses to stress at the protein level. In order to study stress-responsive molecular mechanisms for Populus 9 euramericana cv. '74/76', one of the most important forest plantation tree species in subtropical and temperate regions, we analyzed the response of 2-year-old cuttings of P. 9 euramericana cv. '74/76' to drought and high temperature using twodimensional gel electrophoresis. More than 1,000 reproducible leaf proteins were detected in the controls and treatments, and 26 proteins were found to change notably in abundance. We identified 13 proteins affected by drought stress and 11 proteins affected by high temperature. These proteins are mainly involved in photosynthesis such as ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and putative photosystem I reaction center subunit II precursor, and detoxification (manganese superoxide dismutase and methionine sulfoxide reductase A). Furthermore, the level of the photosynthesis proteins affected greatly by the imposed stress conditions was consistent with the observed noticeable decrease in net photosynthesis rate. These studies provides a fundamental data for future research on responses to drought and high temperature, two major factors limiting the growth of forest trees during summer under recent climatic warming.

Physiological, biochemical, and proteome profiling reveals key pathways underlying the drought stress responses ofHippophae rhamnoides

“…Metabolic processes are necessary for life, and a number of studies have shown that these pathways can be regulated in forest tree species under abiotic stress conditions, involving genes in a number of metabolic processes such as carbon, energy, carbohydrate, and amino acid metabolism . Indeed, in our study, five photosystem II oxygen‐evolving enhancer proteins were downregulated in abundance, causing Pn to be significantly decreased under drought stress (Fig.…”

Evidence of current impact of climate change on life: a walk from genes to the biosphere

“…The individual molecular responses of plants to drought and warming are frequently related to physiological (Xu & Zhou, ; He et al ., ; Aubert et al ., ; Yang et al ., ; Aranjuelo et al ., ), phenological (Swarbreck et al ., ) and anatomical (Spieb et al ., ) responses. Moreover, changes in the molecular composition of plants in response to drought are linked to changes in elemental stoichiometry (Rivas‐Ubach et al ., ) (Fig.…”

“…The mechanisms of molecular responses to warming strongly differ when comparing different plant species, even those belonging to the same genus (Xu & Huang, Fig. 1 Impacts from climate change on life at different spatial scales from the molecular to the biospheric levels. (Xu & Zhou, 2006;He et al, 2008;Aubert et al, 2010;Yang et al, 2010;Aranjuelo et al, 2011), phenological (Swarbreck et al, 2011) and anatomical (Spieb et al, 2012) responses. Moreover, changes in the molecular composition of plants in response to drought are linked to changes in elemental stoichiometry (Rivas-Ubach et al, 2012) ( Fig.…”

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

“…The abundances of Prx and Trx were increased in response to drought, indicating the enhanced detoxification in drought-stressed leaves. Besides, a Trx-linked enzyme, methionine sulfoxide reductase (MSR), was also increased in some plants [57]. MSR is involved in the enzymatic conversion of methionine sulfoxide to methionine, which is involved in the protection of cells and tissues from H 2 O 2 -induced stress [148].…”