Physiological, biochemical, and proteome profiling reveals key pathways underlying the drought stress responses ofHippophae rhamnoides

Abstract

The effects of drought on plant growth and development are occurring as a result of climate change and the growing scarcity of water resources. Hippophae rhamnoides has been exploited for soil and water conservation for many years. However, the outstanding drought-resistance mechanisms possessed by this species remain unclear. The protein, physiological, and biochemical responses to medium and severe drought stresses in H. rhamnoides seedlings are analyzed. Linear decreases in photosynthesis rate, transpiration rate, and the content of indole acetic acid in roots, as well as a linear increase in the contents of abscisic acid, superoxide dismutase, glutathione reductase, and zeatin riboside in leaves are observed as water potential decreased. At the same time, cell membrane permeability, malondialdehyde, stomatal conductance, water use efficiency, and contents of zeatin riboside in roots and indole acetic acid in leaves showed nonconsistent changes. DIGE and MS/MS analysis identified 51 differently expressed protein spots in leaves with functions related to epigenetic modification and PTM in addition to normal metabolism, photosynthesis, signal transduction, antioxidative systems, and responses to stimuli. This study provides new insights into the responses and adaptations in this drought-resistant species and may benefit future agricultural production.

Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins

“…Water deficit results in decrease in abundance of pyruvate dehydrogenase (PDH) subunits [ 19 ]. However, alterations in abundance of Hippophae rhamnoides PDH E1 subunit α in drought [ 65 ] contrasts with our data (decreased in abundance spots No. 223, 241 for “ A ” and “ C ”; Table S1 ).…”

“…We applied functional classification for the clustering of drought-responsive proteins ( Table S5 ). Some functional classes are often underrepresented, highlighting the relevance of organelle-specific studies; on the other hand, key stress-related enzymes (for carbohydrate and amino acid metabolism) are often overrepresented in drought response [ 7 , 45 , 64 , 65 ], whereas proteins related to protein folding and degradation may decline in abundance [ 30 ].…”

Transcriptomics Analyses Reveal Wheat Responses to Drought Stress during Reproductive Stages under Field Conditions

“…This finding differed from those of Hippophae rhamnoides seedlings, where the proteins responsive to drought stress were mainly located in the chloroplast, mitochondria and secretory pathway (He et al, 2016). Plant oxidative stress can be caused by excess H 2 O 2 accumulation under drought stress, which severely damages biomolecules due to the elevated and non-metabolized cellular H 2 O 2 (Sofo et al, 2015).…”

Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry

“…Hierarchical cluster analysis was performed by using pheatmap R package. Relative quantification of 5 key genes in the uronic acid pathway was performed by quantitative RT‐PCR (34) (Supplemental Table 1), and all reactions were performed in triplicate for each sample. Gene expression was quantified relative to 18S rRNA gene expression by using the Δ C t method.…”