Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry

Abstract

Berries of sea buckthorn, known as the "king of vitamin C," are abundant in antioxidants, have attractive colors, and are an excellent material with which to study the relationships between berry color, antioxidants, and berry quality. No study has yet determined the molecular basis of the relationship between sea buckhorn berries and their color and antioxidant levels. By using RNA-seq, LC-MS/MS, and LC/GC-MS technology and selecting red (darkest colored) and yellow (lightest colored) sea buckthorn berries at different development stages, this study showed that the red and yellow berry resulted from a higher ratio of lycopene to β-carotene and of β-carotene to lycopene content, respectively. The uronic acid pathway-a known animal pathway-in ascorbic acid synthesis was found in sea buckthorn berries, and the higher expression of UDP-glucuronosyltransferase in red berries was consistent with the higher content of ascorbic acid. In summary, multiomic data showed that the color of sea buckthorn berries is mainly determined by β-carotene and lycopene; red sea buckthorn berries were richer than yellow berries in antioxidants, such as carotenoids, flavonoids, and ascorbic acid; and the animal pathway might be operating in sea buckthorn.-He, C., Zhang, G., Zhang, J., Zeng, Y., Liu, J. Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry.

Genome-wide organization and expression profiling of the R2R3-MYB transcription factor family in sea buckthorn (Hippophae rhamnoides L.)

“…The expression levels of gene HrMYB157 peaked at first stage and gene HrMYB104 peaked at second stage, subsequently, their expression levels gradually decreased with fruit development. This trend was in line with the change of flavonoids content during sea buckthorn fruit development [52]. In addition, gene HrMYB11, HrMYB14, HrMYB19, HrMYB107 and HrMYB58 had higher expression levels at the first stage and lower at the second or third stage.…”

Genome sequence and population genomics provide insights into chromosomal evolution and phytochemical innovation of Hippophae rhamnoides

“…L-Galactose pathway is involved in the high accumulation of ascorbic acid in seabuckthorn Richness in AsA was another essential feature of seabuckthorn fruit (He et al, 2017), and the AsA content in pulp decreased sharply following the maturation of seabuckthorn fruit (Figure 5d). Comparative genomics and transcriptome analysis were used to explore the molecular basis underlying its biosynthesis (Figures 5e,g and S16).…”

“…Seabuckthorn fruits were collected at 46, 63, and 76 days postanthesis. Library construction and RNA-seq were performed according to He et al (2017). TopHat2 (Kim et al, 2013) and Cuffdiff were used to map the clean reads to the reference genome and calculated expression level, respectively.…”

West meets east: open up a dialogue on phytomedicine

“…2 B), which are higher than that in any other ones. Besides, sea buckthorn berries are called “king of vitamin C” as the vitamin C content is claimed to be among the richest content in all the fruits and vegetables [ 48 , 51 , 52 ]. These bioactive compounds that the sea buckthorn contains also contribute to its medicinal properties.…”