Proteins in pepper plants (“Capsicum annuum” L.) under heat stress

Turhan, Ece; Aydogan, Cigdem; Ergin, Sergül; Ozturk, Nilufer

doi:10.1016/j.jbiotec.2015.06.085

Cited by 5 publications

(6 citation statements)

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“…Tokyol and Turhan (2019) reported that in 'Balkız', 'Ferasetsiz' and 'Local Genotype' green bean genotypes, the TSP content reduced and/or increased depending on the temperature and genotypes. Similarly, Turhan et al (2015) reported that heat stress application increased the TSP content up to certain temperatures in pepper cultivars. Ergin et al (2016) also determined that TSP content decreased, while new proteins had been synthesized depending on increased temperatures in strawberry cultivars.…”

Section: Figure 1 Effect Of Heat Treatments On the Leaf Chlorophyll (Chl) (Panel A) And Carotenoid (Car) (Panel B)contents Of Tomato Cultmentioning

confidence: 88%

“…It was declerated that new proteins are synthesized or reduced and almost complete lost in tomatoes in response to high temperature (Heckathorn et al, 1998). In addition, Turhan et al (2015) found that strips of 7-54 kDa in the pepper plant under high temperature stress conditions and 40 kDa protein band may be associated with high temperature tolerance. The preservation of protein structures and functions under stress conditions is very important for the survival of the cell (Wang et al, 2004).…”

Section: Figure 1 Effect Of Heat Treatments On the Leaf Chlorophyll (Chl) (Panel A) And Carotenoid (Car) (Panel B)contents Of Tomato Cultmentioning

confidence: 96%

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Physiological Effects of High Temperature Treatments on Tomato Leaves at Two Developmental Phases

Turhan¹,

Aydogan²,

Ergin³

2021

CTNS

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This study was conducted to investigate the effects of high temperatures on three tomato cultivars at first bloom and yield stages. The leaves were subjected to high temperature stress at 35, 40, 45, 50, 55 and 60°C with gradual increments every 30-minutes in both stages. Samples were analyzed for total chlorophyll (Chl), carotenoid (Car), ascorbic acid (AsA), glutathione (GSH), total soluble protein (TSP) contents. Besides, protein profiles were determined with SDS-PAGE. Heat stress decreased Chl content in both stages, while it was higher in first bloom stage than in yield stage. Whereas carotenoid content increased in both stages. The AsA and GSH contents were higher in yield stage than in first bloom stage. Heat stress, generally reduced AsA content, while increased GSH content. It was observed that the effect of cultivars and temperature treatments on the TSP content was different in both periods. In addition, TSP content had decreased with increasing temperatures, while many protein bands had been observed in SDS-PAGE with sizes ranging from 13 kDa to 89 kDa according to treatments.

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Section: Figure 1 Effect Of Heat Treatments On the Leaf Chlorophyll (Chl) (Panel A) And Carotenoid (Car) (Panel B)contents Of Tomato Cultmentioning

confidence: 88%

Section: Figure 1 Effect Of Heat Treatments On the Leaf Chlorophyll (Chl) (Panel A) And Carotenoid (Car) (Panel B)contents Of Tomato Cultmentioning

confidence: 96%

Physiological Effects of High Temperature Treatments on Tomato Leaves at Two Developmental Phases

Turhan¹,

Aydogan²,

Ergin³

2021

CTNS

Self Cite

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“…New proteins are synthesized in response to high temperature and there are decreases and almost complete disappearance of proteins due to high temperatures in strawberry plants (Ergin et al, 2016). Turhan et al (2015) detected bands ranging in size from 7-54 kDa in pepper plants under high temperature stress conditions and pointed out that 40 kDa HSP protein may be associated with tolerance to high temperature. In this study, it has been found that TSP content does not play an important role in tolerating high temperatures in cucurbits.…”

Section: Figure 3 Effects Of High Temperature Stress On Rwc (%) Of Cucurbit Plants Vertical Bars Indicate ± Sementioning

confidence: 99%

“…The synthesis of new proteins or the decrease in their synthesis in plants exposed to stress, and the destruction of existing proteins are shown as a defence mechanism against stress. Total soluble proteins are among the compounds that show the greatest variation under stress conditions (Turhan et al, 2015;Ergin et al, 2016). Cucumber (Cucumis sativus L.), zucchini (Cucurbita pepo L.), melon (Cucumis melo L.) and watermelon (Citrullus lanatus) are important crop for their nutritional properties and benefits for human health, that belonging to the family of Cucurbitaceae.…”

Section: Introductionmentioning

confidence: 99%

Physiological Effects of High Temperature Stress in Some Cucurbit Plants

Ergin¹,

Aydogan²,

Turhan³

2021

CTNS

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Some cucurbit plants (Cucumis sativus L., Cucurbita pepo L., Cucumis melo L. and Citrillus lanatus L.) were used for investigating some physiological changes and protein patterns under heat stress in plants. Leaf samples were exposed tohigh temperatures as follows 35, 40, 45, 50, 55 and 60°C. Percentage of ion leakage, chlorophyll content, leaf relative water content (RWC) and loss of turgidity, total soluble protein (TSP) content and protein profiles were determined in leaf samples kept at each temperature stage for 30 minutes. Ion leakage was increased especially 55 and 60°C in all cucurbits parallel to the temperature increasement. Total chlorophyll content was decreased slightly in both cucumber cultivars, zucchini, in both melon cultivars and watermelon due to high temperatures. The RWC of cucurbits was decreased on the contrary of the loss of turgidity, which was parallel to the increment of temperatures. While the TSP content in cucumber was decreased with temperature increasement, it was not changed in zucchini and watermelon. In melon cultivars TSP was not noticeably changed until 55°C, but significantly decreased at 60°C. When the SDS-PAGE profiles of cucurbits were examined, many protein bands were observed with sizes ranging from 6.5 kDa to 211 kDa.

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“…Besides, a new protein band with a molecular weight of 44 kDa was observed only at 50°C high temperature treatment. Turhan et al (2015) determined bands in the range of 7-54 kDa in pepper plants due to heat stress and pointed out that 40 kDa HSP protein may be associated with thermal tolerance. Likewise, in tomato plants protein bands ranging between 17-89 kDa (at first bloom stage) and 13-89 kDa (at yield stage) were detected, of which a protein band of 43 kDa was found to be only in cv.…”

Section: Figure 3 Effects Of Heat Stress On Acidic (Panel A) and Basi...mentioning

confidence: 99%

Effects of Heat Stress on Some Biochemical Traits of Small Reddish Bean

Aydogan¹,

Turhan²

2022

CTNS

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The effects of heat stress on malondialdehid (MDA) content, ascorbic acid (AsA), isoperoxidases, total soluble protein (TSP) and SDS-PAGE protein profiles of small reddish bean (Phaseolus vulgaris L. cv. Keklik) leaves were investigated. For this purpose, the collected leaf samples (preferably the third from the apex) were placed into pyrex tubes with caps closed and incubated in a water bath: after a 30-minutes habituation at 30ºC, to apply heat stress the leaves were subjected to 35, 40, 45, 50, 55 and 60°C with gradual increments every 30-minutes. According to the results, the MDA content increased with escalating temperatures, starting with 40°C. Although there was fluctuation, the AsA content generally decreased with high temperatures. Besides, TSP content decreased after 50°C. Moreover, three asidic and four basic isoperoxidase bands were identified. There was a marked difference at acidic isoperoxidase band intensities under 40 and 45°C heat treatment. Protein bands with molecu1ar weight of approximately 14.5-195 kDa were determined. In conclusion, especially 40°C is critical for small reddish bean plants, besides 40°C and above temperatures causes oxidative stress in small reddish bean plant. Additionally, isoperoxidases and proteins may be an effective mean on heat stress tolerance.

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Proteins in pepper plants (“Capsicum annuum” L.) under heat stress

Cited by 5 publications

References 0 publications

Physiological Effects of High Temperature Treatments on Tomato Leaves at Two Developmental Phases

Physiological Effects of High Temperature Treatments on Tomato Leaves at Two Developmental Phases

Physiological Effects of High Temperature Stress in Some Cucurbit Plants

Effects of Heat Stress on Some Biochemical Traits of Small Reddish Bean

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