Physiological Effects of High Temperature Treatments on Tomato Leaves at Two Developmental Phases

Abstract

This study was conducted to investigate the effects of high temperatures on three tomato cultivars at first bloom and yield stages. The leaves were subjected to high temperature stress at 35, 40, 45, 50, 55 and 60°C with gradual increments every 30-minutes in both stages. Samples were analyzed for total chlorophyll (Chl), carotenoid (Car), ascorbic acid (AsA), glutathione (GSH), total soluble protein (TSP) contents. Besides, protein profiles were determined with SDS-PAGE. Heat stress decreased Chl content in both stages, while it was higher in first bloom stage than in yield stage. Whereas carotenoid content increased in both stages. The AsA and GSH contents were higher in yield stage than in first bloom stage. Heat stress, generally reduced AsA content, while increased GSH content. It was observed that the effect of cultivars and temperature treatments on the TSP content was different in both periods. In addition, TSP content had decreased with increasing temperatures, while many protein bands had been observed in SDS-PAGE with sizes ranging from 13 kDa to 89 kDa according to treatments.

Effects of Heat Stress on Some Biochemical Traits of Small Reddish Bean

“…Tokyol and Turhan (2019) found that the TSP content of green bean genotypes reduced and/or induced with heat stress. Besides, in tomato (Turhan et al, 2021) and cucumber (Ergin et al, 2021) plants fluctuations in the TSP content under high temperatures were reported. Besides, the TSP contents of zucchini plants were not affected by the temperature, while the TSP content of melon plants did not change up to 50°C and then decreased with the increase in temperature (Ergin et al, 2021).…”

“…Likewise, in tomato plants protein bands ranging between 17-89 kDa (at first bloom stage) and 13-89 kDa (at yield stage) were detected, of which a protein band of 43 kDa was found to be only in cv. 'Yaren' (relatively tolerant) at yield stage (Turhan et al, 2021). Besides, Ergin et al (2016) indicated that heat stress resulted in new protein bands and decreases/disappearance of some proteins in strawberry plants.…”

“…11, Issue 22, pp. 153-161, 2022 https://doi.org/10.47068/ctns.2022.v11i22.018 Current Trends in Natural Sciences (on-line) Current Trends in Natural Sciences (CD-Rom) ISSN: 2284-953X ISSN: 2284-9521 ISSN-L: 2284-9521 ISSN-L: 2284-9521 http://www.natsci.upit.ro *Corresponding author, E-mail address: ciaydogan@ogu.edu.tr 157 first bloom stage(Turhan et al, 2021). The leaf AsA content of melon and watermelon showed fluctuation with increasing temperatures(Aydogan et al, 2021).…”