Proteins in Human Tears

Sapse, A.T.; Bonavida, B; Stone, W. H.; Sercarz, Eli E.

doi:10.1001/archopht.1969.00990010817012

Cited by 43 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It contains various chemicals such as glucose (Ridley 1930;Giardini and Roberts 1950;Hill 1982, 1984), ascorbic acid (Kuizenga et al 1987;Paterson and O'Rourke 1987;Choy et al 2000;Bilgihan et al 2001) and various proteins (e.g. albumin (Sapse et al 1969;Li et al 2005), lysozyme (Mackie and Seal 1976;Aviser et al 1979;Farris 1985), epidermal growth factor (Wilson 1991;Van setten et al 1991;Pflugfelder et al 1999;Ohashi et al 2003)). Tear contents are known to reflect physiological conditions.…”

mentioning

confidence: 99%

Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents

Chu

Shirai

Takahashi

et al. 2011

Biomed Microdevices

View full text Add to dashboard Cite

A soft contact-lens biosensor (SCL-biosensor) for novel non-invasive biomonitoring of tear fluids was fabricated and tested. Wearing a biosensor on eye enabled the in situ monitoring of tear contents. The biosensor has an enzyme immobilized electrode on the surface of a polydimethyl siloxane (PDMS) contact lens. The SCL-biosensor was fabricated using microfabrication techniques for functional polymers (PDMS and 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer). In investigation of in vitro characterization, the SCL-biosensor showed excellent relationship between the output current and glucose concentration from 0.03 to 5.0 mmol·L(-1), with a correlation coefficient of 0.994. The calibration range covered the reported tear glucose concentrations (0.14 mmol·L(-1)). Based on the result, ocular biomonitoring with the SCL-biosensor was carried out. The SCL-biosensor well worked both in the static state and the dynamic state. The tear glucose level of rabbit was estimated to 0.12 mmol·L(-1) at first and then the tear turnover was successfully calculated to be 29.6 ± 8.42% min(-1). The result indicated that SCL-biosensor is useful for advanced biomonitoring on eye.

show abstract

mentioning

confidence: 99%

Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents

Chu

Shirai

Takahashi

et al. 2011

Biomed Microdevices

View full text Add to dashboard Cite

show abstract

“…It was previously shown that serum albumin is in trace amounts in unstimulated tears under typical conditions but increases in tear concentration in any mechanical damage (18). Josephson et al also found higher levels of albumin, beta globulin, and transferritin concentrations in tears taken after rubbing the eyes for 3 minutes than their serum levels and in the other non-traumatized eye.…”

Section: Discussionmentioning

confidence: 92%

The impacts of contact lenses on the short- and long-term biochemical parameters of tear films and corneal thickness

CAN¹,

İrkeç²

2022

J. Transl. Pract. Med.

View full text Add to dashboard Cite

Introduction: To investigate the effects of long- and short-term use of different types of contact lenses on corneal metabolism and corneal thickness. Materials and Method: The participants were divided into two groups in the study. In the first group, 20 of the 42 patients were administered a rigid gas-permeable lens (RGPL) with a Dk value of 92x10-11, while the remaining wore a soft contact lens with a water content of 38%. We then explored their Lactate Dehydrogenase (LHD) levels to evaluate the hypoxic effect on tear films in short- (8 hours) and long-term (2nd, 3rd, 5th months) and albumin levels to assess mechanical trauma. In the second group, the corneal thickness was measured using a Haag-Streit optical pachymeter before and at the 8th hour and 2nd month following the first set of lenses, and LDH and albumin values were simultaneously evaluated in tear film. Results: The results yielded no significant increase in tear film LDH and albumin levels in the short- and long-term in both the RGPL and the soft contact lens groups with similar age and sex distribution. In the second part, the corneal thickness measurements resulted in an increase of 5.5% in the RGPL group and 3.63% in the soft lens group at the 8th hour of lens use. However, the groups did not significantly differ by baseline corneal thickness at the 2nd-month follow-up. Similarly, there was no significant increase in LDH and albumin levels in the tear film samples of the same group. Conclusion: The absence of a long-term increase in corneal thickness and no elevated levels of albumin and LDH in tear films indicate that contact lenses fit corneal physiology.

show abstract

“…Some of them are always present (IgG, albumin, alpha-l-antitrypsin, alpha-1-antichymotrypsin, transferrin, beta-2-microglobulin), and others appear sporadically (cerulcoplasmin. haptoglobin, zinc aIpha-2-glycoprotein) [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Functional Exploration of the Lacrimal Gland by Tear Electrophoresis

Liotet¹,

Warnet²,

Arrata³

1982

Ophthalmologica

View full text Add to dashboard Cite

Electrophoresis of tears, a rapid and easy technique, shows several peaks of migration. Three main peaks correspond to the proteins synthesized by the lacrimal gland: rapid migrant proteins, lactoferrin migrating to the anode, and lysozyme migrating to the cathode. Secretory tissue injuries of the lacrimal gland show a more or less important decrease of one or several peaks. Any inflammatory reaction induces an increase in the serum albumin and immunoglobulin fractions. This exploration can be helpful in the diagnosis of the sicca syndrome or of drug poisoning thereby avoiding lacrimal biopsy.

show abstract

Proteins in Human Tears

Cited by 43 publications

References 13 publications

Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents

Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents

The impacts of contact lenses on the short- and long-term biochemical parameters of tear films and corneal thickness

Functional Exploration of the Lacrimal Gland by Tear Electrophoresis

Contact Info

Product

Resources

About