Proteinhydrolysis: A Description of the Method Used at the Department of Animal Physiology in Copenhagen

Weidner, Kirsten; Eggum, B. O.

doi:10.1080/00015126609434171

Cited by 111 publications

(29 citation statements)

References 4 publications

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“…Acetone was added till precipitate was formed and precipitated by centrifugation at 3000 r.p.m then collected, weighted and dried. The HPLC analysis of amino acids was performed using a model Eppendorf-Germany LC 3000 amino acid analyser (Widner and Eggum 1966). …”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride

et al. 2018

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The aim of the present study was to evaluate different biological activities of Trichoderma viride fungus (Family Hypocreaceae). Trichoderma viride isolated for the first time from the cucumber soil (rhizosphere). It was tested as antimicrobial, antioxidant and anticancer agent. Trichoderma viride from the cucumber soil (rhizosphere) caused inhibition of the mycelial growth of Fusarium solani, Rhizoctonia solani and Sclerotium rolfsii. Also, the alcoholic extract of the fungal mycelia proved a potent antibacterial activity against Bacillus subtilis, Escherichia coli and Pseudomonas fluorescens. In addition, it exhibited a significant antifungal activity against Candida albicans, Fusarium solani, Fusarium oxysporium, Rhizoctonia solani and Pythium ultimum at 100 µg/disc. Study of the antimicrobial and antioxidant activities of the volatile constituents had been done. The in vitro antioxidant, anticancer and antiviral activities of the isolated proteins, and carbohydrates were determined. Furthermore, the volatile constituents were isolated from fresh mycelia of Trichoderma viride and subjected to GC/MS analysis. Total protein (10%), carbohydrate (19.57%), steroidal (13.95%) and triterpenoidal content (38.34%) were determined in the alcoholic extract of Trichoderma viride mycelia. In conclusion, this fungus showed antioxidant, anticancer, antiviral and antibacterial effects. Further studies must be done to identify the molecules responsible for its effect and to consider its application in the pharmacological and medicinal purposes.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride

et al. 2018

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“…Norleucine served as an internal standard. Cysteine and methionine, which are destroyed during the acid hydrolysis, were converted to acid-stable derivatives (cysteinic acid and methioninesulfone, respectively) by performic acid oxidation [21]. The oxidized samples were then hydrolysed with 6 N HCI as described above.…”

Section: Methodsmentioning

confidence: 99%

Amino acid composition, available lysine content and in vitro protein digestibility of selected tropical crop seeds

Petzke

Ezeagu

Proll

et al. 1997

Plant Food Hum Nutr

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As the search for alternative sources of food to alleviate hunger continues, this study was undertaken to determine nitrogen and amino acid content, chemical score, protein digestibility corrected amino acid score, available lysine and in vitro digestibility of 8 lesser known, wild tropical seeds, gathered in Nigeria. Results were contrasted with a tropical soybean variety (Glycine max, TGX 1660-15F). The investigated seeds were Millettia thonningii, Gliricidia sepium, Lonchocarpus sericeus, Albizia zygia, Daneillia ogea and Afzella bella from the family of Leguminosae, Diospyros mespiliformis (Ebenaceae) and Entandrophragma angolense (Meliaceae). The crude protein content, based on nitrogen determination, was found to be lower in the wild seeds compared to soybean, which was partly due to the relatively high content of non-protein nitrogen. With reference to amino acid requirement and digestibility in most seed samples, lysine, followed by sulphur amino acids and threonine, were the limiting amino acids. It was concluded, that these less familiar wild seed plants may be used as valuable food or feed complements. However, further investigation is necessary to elucidate potential toxic and antinutritional factors.

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“…Amino acid in the hydrolysates were determined on a Beckman Amino Acid Analyser, Model 120°C, by using sodium-based buffer solutions. Methionine and cystine were determined as methionine sulphone and cysteic acid after oxidising the samples with performic acid at 50°C for 15 min and subsequent hydrolysis according to a method by Moore (1963) as modified by Weidner and Eggum (1966).…”

Section: Methodsmentioning

confidence: 99%

Free and Total Amino Acid Composition of Edible Parts of Beans, Kale, Spinach, Cauliflower and Potatoes as Influenced by Nitrogen Fertilisation and Phosphorus and Potassium Deficiency

Eppendorfer¹,

Bille²

1996

J. Sci. Food Agric.

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Proteinhydrolysis: A Description of the Method Used at the Department of Animal Physiology in Copenhagen

Cited by 111 publications

References 4 publications

Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride

Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride

Amino acid composition, available lysine content and in vitro protein digestibility of selected tropical crop seeds

Free and Total Amino Acid Composition of Edible Parts of Beans, Kale, Spinach, Cauliflower and Potatoes as Influenced by Nitrogen Fertilisation and Phosphorus and Potassium Deficiency

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