Amino acid composition, available lysine content and in vitro protein digestibility of selected tropical crop seeds

Abstract

As the search for alternative sources of food to alleviate hunger continues, this study was undertaken to determine nitrogen and amino acid content, chemical score, protein digestibility corrected amino acid score, available lysine and in vitro digestibility of 8 lesser known, wild tropical seeds, gathered in Nigeria. Results were contrasted with a tropical soybean variety (Glycine max, TGX 1660-15F). The investigated seeds were Millettia thonningii, Gliricidia sepium, Lonchocarpus sericeus, Albizia zygia, Daneillia ogea and Afzella bella from the family of Leguminosae, Diospyros mespiliformis (Ebenaceae) and Entandrophragma angolense (Meliaceae). The crude protein content, based on nitrogen determination, was found to be lower in the wild seeds compared to soybean, which was partly due to the relatively high content of non-protein nitrogen. With reference to amino acid requirement and digestibility in most seed samples, lysine, followed by sulphur amino acids and threonine, were the limiting amino acids. It was concluded, that these less familiar wild seed plants may be used as valuable food or feed complements. However, further investigation is necessary to elucidate potential toxic and antinutritional factors.

Chemical composition, nutritive value and voluntary intake of tropical tree foliage and cocoyam in pigs

“…Many tropical feedstuffs present a deficiency in one or more major amino acids, generally lysine and methionine. 10,17 The comparison with the requirements of pigs weighing 50-80 kg and those of lactating sows demonstrates that lysine is limiting for Trichanthera only. In all the other cases, the requirements in the most limiting factors, ie lysine, methionine-cysteine, threonine and tryptophan, were covered.…”

Comparative Phytochemical, Antioxidant and Antimicrobial Properties of Ficus capensis, Aristolochia ringens, Albizia zygia and Lannea welwitschii

“…It is deciduous tree with a deciduous crown. 29 The plant is used in the treatment of allergycaused respiratory disorders 30 and diabetes 31 and a variety of other conditions in Africa such as cough, fever, worms and female infertility.…”

Farinha integral de trigo germinado: 3. Características nutricionais e estabilidade ao armazenamento

“…Foi calculado em porcentagem, conforme PETZKE et al [26], multiplicando-se o escore químico (EQ) do aminoácido limitante pela digestibilidade in vitro e dividindo o resultado por 100.…”

“…PETZKE et al [26] também encontraram resultados de disponibilidade de lisina superestimados (de 115 a 233%) em sementes da família das leguminosas, o que atribuíram a fatores da matriz da amostra relacionados aos constituintes carboidratos, que quando aquecidos com ácido clorídrico (HCl), produzem furfural, que pode ser o agente redutor responsável pela perda de grupos ε-amino. Ah v uhÃD rt hy …”

“…Contudo, BOUTRIF [5] sugere que procedimentos promissores de digestibilidade in vitro sejam aperfeiçoados e avaliados. PETZKE et al [26] empregaram a digestibilidade protéica in vitro para esse cálculo, baseados em AKESON & STAHMANN [1], que demonstraram ser análogos aos resultados obtidos por métodos in vivo aplicados em ratos. O PDCAAS da farinha integral controle obtido no presente trabalho foi 42%, bem próximo do valor obtido por HENLEY & KUSTER [13], que citam 0,40 ou 40% para o PDCAAS da proteína do grão de trigo.…”