Proteinase inhibitor in human tears

Quantitative Reverse Zymography: Analysis of Picogram Amounts of Metalloproteinase Inhibitors Using Gelatinase A and B Reverse Zymograms

“…The technique has its origin in a fibrinogen-agarose plate assay for protease inhibitory activity in which a digesting agent was added to grooves after electrophoresis and allowed to diffuse into the gel (10). The original method had very crude sensitivity and resolving power by current standards but was somewhat improved with a complete submersion technique developed later (11). Fibrinogen-agarose electrophoresis was later described for the detection of protease inhibitors using crude horse serum as a source of inhibitors (12).…”

Connective tissue growth factor in tear film of the horse: detection, identification and origin

“…The simultaneous presence of enzymes in corneas with corneal ulceration possibly may also contribute to reduced CTGF levels in tears due to proteolysis. The importance of proteolytic enzymes and their regulatory inhibitors in tears has been investigated extensively [1,2,10,11,12,19,20,21,22,23,24,25,26,27].…”

Structure basis 1/2SLPI and porcine pancreas trypsin interaction

“…It inhibits various serine proteases, including elastase, cathepsin G, chymotrypsin and trypsin (Seemü ller et al, 1986;Smith & Johnson, 1985;Thompson & Ohlsson, 1986). SLPI has been found in a variety of fluids, including saliva (Ohlsson et al, 1983), bronchi mucus (Ohlsson et al, 1977), tears (Kueppers, 1971), cervical mucus (Wallner & Fritz, 1974) and seminal plasma (Schiessler et al, 1976). In the respiratory tract, SLPI is produced by serous cells of tracheal and bronchial submucosal glands and by non-ciliated bronchiolar epithelial cells, identified as goblet and clara cells (Kramps et al, 1981(Kramps et al, , 1989Willems et al, 1986).…”