Proteinase inhibitor II gene in transgenic poplar: Chemical and biological assays

Klopfenstein, Ned B.; Allen, Kurt K.; Ávila, Francisco; Heuchelin, Scott A.; Martinez, J.A.; Carman, Richard C.; Hall, Richard B.; Hart, E. R.; McNabb, Harold S.

doi:10.1016/s0961-9534(97)00004-4

Cited by 23 publications

(10 citation statements)

References 43 publications

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“…Protease inhibitors play an endogenous role in preventing uncontrolled proteolysis and/or in protecting against the foreign proteolytic enzymes of pests or pathogens [5,6]. Observations of their woundinducible expression [7,8] have led to investigations focusing on their role in plant protection against insects [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

et al. 2015

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“…The Bt gene has been introduced into several other tree species like walnut and white spruce , Dandekar et al 1998. As a different approach to engineer insect resistance, proteinase inhibitors have been expressed in poplar (Leplé et al 1992, Klopfenstein et al 1997. Increased resistance against the fungal pathogen Septoria musiva has been achieved in poplar by expression of chitin binding peptides .…”

Section: Biotic Stressmentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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“…Furthermore, lettuce neither possesses detectable trypsin inhibitory activity in its leaves nor responds to any treatments by accumulating inhibitors (WalkerSimmons and Ryan, 1977). Since the over-expression of potato and tomato PIN2 conferred insect resistance (Johnson et al, 1989;Duan et al, 1996;Klopfenstein et al, 1997), production of insectresistant lettuce would be desirable as it is an economically important vegetable crop grown globally (Ryder, 1999).…”

Section: Expression Of Sapin2a Mrna In Transgenic Lettuce Inhibits Plmentioning

confidence: 99%

Serine proteinase inhibitor proteins: Exogenous and endogenous functions

Chye

Sin

et al. 2006

In Vitro Cell. Dev. Biol. - Plant

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SummaryProteinase inhibitor II (PIN2) proteins from the Solanaceae family have been previously used in plant transformation to acquire protection against caterpillars. Some of these PIN2 proteins have been shown to exhibit exogenous activities against trypsin and/or chymotrypsin in vitro. Despite their application in conferring insect resistance in transgenic plants, the endogenous roles of this family of proteins in various plant species have not been well defined. To investigate the exogenous and endogenous functions of PIN2 proteins, cDNAs encoding PIN2 proteins from the weed Solanum americanum (American black nightshade), designated SaPIN2a and SaPIN2b, were cloned and characterized. The localization of S. americanum SaPIN2a and SaPIN2b mRNAs and proteins in the reproductive tissues destined to undergo developmental programmed cell death subsequently led to investigations into their function during seed development. Using plant transformation of lettuce and S. americanum, it was evident that: (1) the expression of SaPIN2a in transgenic lettuce conferred resistance to cabbage looper (Trichoplusia ni) caterpillars; and (2) the expression of siRNAs from a PIN2-RNAi construct resulted in transgenic S. americanum that were impaired in seed development. These results suggest that S. americanum PIN2 proteins not only enhance resistance to caterpillars (when expressed exogenously) but they function in inhibiting endogenous proteases that are expressed during seed development. Specifically, the aborted seeds of PIN2-RNAi lines showed abnormal endothelium that subsequently affected endosperm and embryo development.

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Proteinase inhibitor II gene in transgenic poplar: Chemical and biological assays

Cited by 23 publications

References 43 publications

Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Wood production, wood technology, and biotechnological impacts

Serine proteinase inhibitor proteins: Exogenous and endogenous functions

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