Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Gandreddi, V D Sirisha; Kappala, Vijaya Rachel; Zaveri, Kunal; Patnala, Kiranmayi

doi:10.1186/s12858-015-0052-7

Cited by 10 publications

(6 citation statements)

References 52 publications

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“…With regards to mechanism of action, SMTI showed a non-competitive type of inhibition similar to that reported in Sapindus trifoliatus seeds 12 . Vogel et al .…”

Section: Discussionsupporting

confidence: 77%

“…A trypsin inhibitor from S. mukorossi seeds (SMTI) was purified with a recovery of 10.47% and purification fold of 9.26 which was greater than the trypsin inhibitor purified from Inga vera seeds 11 (yield 7.14%; purification fold 1.36) and Lonchocarpus sericeus seeds 10 (yield 0.88%; purification fold 8.26). SDS-PAGE depicted a molecular mass of ~29 kDa, similar to that of other trypsin inhibitors 12,13 .…”

Section: Discussionmentioning

confidence: 59%

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Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Samiksha

Singh

Kesavan

et al. 2019

Sci Rep

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Peptidase inhibitors (PIs) are defense proteins of plants which are active against gut peptidases of different insects. Sapindus mukorossi was identified as a source of bioactive PIs which could confer resistance against Bactrocera cucurbitae, a most devastating pest of several economically important crops. In the present study, a trypsin inhibitor was purified from mature dry seeds of S. mukorossi and characterized for its biochemical properties as well as its potential for bio control of B. cucurbitae. The purified fractions from RP- HPLC through SDS-PAGE gave an apparent molecular weight of ~29 kDa. S. mukorossi trypsin inhibitor (SMTI) was found to be a non-competitive inhibitor which was active over a broad range of temperature (10–100 °C) and pH (6–11). SMTI when incorporated in artificial diet inhibited the growth and development of B. cucurbitae larvae. Gene expression analysis of trypsin and chymotrypsin genes via qRT-PCR indicated that their mRNA expression was down-regulated while that of other genes namely, Catalase, Elastase, Superoxide Dismutase, Glutathione –S-transferase and Alkaline Phosphatase was up regulated. SMTI also showed deleterious effects against different bacterial strains. The results of this study indicated that S. mukorossi trypsin inhibitor has potential to be used as a bio control agent that can reduce the harm caused by melon fruit fly and other devastating pests.

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“…With regards to mechanism of action, SMTI showed a non-competitive type of inhibition similar to that reported in Sapindus trifoliatus seeds 12 . Vogel et al .…”

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 59%

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Samiksha

Singh

Kesavan

et al. 2019

Sci Rep

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show abstract

“…Maximum trypsin and chymotrypsin inhibitory activity was reported from the seeds of S. mukorossi which belongs to sapindaceae family. Gandreddi et al (2015) isolated and purified trypsin inhibitor from soap nut ( Sapindus trifoliatus L. Var. Emarginatus ) seeds and evaluated its role against larval gut peptidases of Helicoverpa armigera and Spodoptera frugiperda .…”

Section: Discussionmentioning

confidence: 99%

“…PIs serve various functions viz. signal initiation mediator, cellular event's transmission and termination processes like apoptosis, inflammatory response, blood coagulation, hormone response pathwayetc (Gomes et al, 2011). They regulate the development of insects, agricultural pests, plant and animal health (Shamsi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

In vitro screening of peptidase inhibitory activity in some plants of North India

Samiksha

Sohal

2020

Heliyon

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In the present study, trypsin and chymotrypsin inhibitory activity of some plants of different families was evaluated. A total of 55 plants were screened, out of which six showed the maximum trypsin inhibitory activity namely Acacia concinna, Caesalpinia bonducella, Lathyrus sativus, Mucuna pruriens, Psoralea corylifolia and Sapindus mukorossi . Results suggested that the plants showing trypsin inhibitory activity (TIA) also have chymotrypsin inhibitory activity (CIA). Both trypsin and chymotrypsin inhibitory activities were high in seeds compared to leaves followed by flowers. It was also observed that TIA was maximally present in Sapindaceae family whereas CIA was maximum in fabaceae family followed by others.

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“…The seed extract and leaf extract of Annona muricata showed trypsin inhibition of 51.8 ± 1.3% and 44.9 ± 1.3% respectively (Table 1). Protease inhibitor activities were reported from the seed extract of Annona muricata [26] . Leaf extract of Murraya koenijii showed 49.9 ± 0.3% trypsin inhibition (Table 1) and Shee and Sharma purified a 27 kDa trypsin inhibitor from the seeds of M. koenijii [27] .…”

Section: Trypsin Inhibition By Plant Extractsmentioning

confidence: 99%

Toxicity of plant extracts containing trypsin inhibitor to the larvae of Aedes aegypti

Aparna¹,

Parambil²,

George³

et al. 2021

Int. J. Mosq. Res.

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Mosquitoes are vectors of many pathogens and can transmit a range of potentially fatal diseases. Some of these diseases spread via mosquitoes belonging to the genus Aedes include Dengue, Chikungunya, Yellow fever, Zika etc. Conventional methods of mosquito control are not always completely successful. Therefore new strategies need to be adopted for mosquito control. Plant protease inhibitors are promising candidates as they prevent larval growth by inhibiting gut proteases of mosquito larvae. In this study we screened 15 plant extracts containing trypsin inhibitors for their toxicity to the larvae of Aedes aegypti. Leaf extracts from Lawsonia inermis showed the highest mortality with 100% mortality after 48 hours in second, third and in fourth instar larvae. The treatment with extract from Annona muricata leaf led to 100, 96 and 66% mortality after 48 hours in second, third and fourth instar larvae respectively. After Proteinase K treatment of Lawsonia inermis leaf extract, a reduction in the in vitro inhibition of larval gut protease activity from 82 to 6% and a decrease in mortality from 80 to 50% were observed compared to untreated. This revealed that the mortality, at least in part, is due to the presence of a protenaceous plant protease inhibitor in the extract. Expressing the gene coding for such protease inhibitors in micro organisms or algae upon which the mosquito larvae feeds will serve as an alternate method for control of mosquito population.

show abstract

Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Abstract: Background: The defensive capacities of plant protease Inhibitors (PI) rely on inhibition of proteases in insect guts or those secreted by microorganisms; and also prevent uncontrolled proteolysis and offer protection against proteolytic enzymes of pathogens.

Cited by 10 publications

References 52 publications

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

In vitro screening of peptidase inhibitory activity in some plants of North India

Toxicity of plant extracts containing trypsin inhibitor to the larvae of Aedes aegypti

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