Proteinase inhibition and antioxidant activity of selected forage crops

Abstract

The hypothesis of the possible therapeutic potential of selected species of forage crops is discussed. Extracts from genotypes of Anthyllis sp., Astragalus sp., Coronilla sp., Lotus sp., Medicago sp., Melilotus sp., Onobrychis sp. and Trifolium sp. were prepared and tested for proteinase inhibition and antioxidant activities. We found that Trifolium pratense accession POLKIE99-3 expressed the highest relative trypsin inhibition activity (80.0%) compared to standards. The highest thrombin inhibition activity (81.4%) was detected in the Medicago sativa old cultivar Hodonínka, whereas the highest relative urokinase inhibition activity (62.5%) was expressed by the local population Nitranka. Relatively high antioxidant properties of Trifolium sp. accessions, Trifolium pratense genetic resources SVKZAH98-40, were of interest. Results of this study confirmed that there are significant differences in proteinase inhibition and antioxidant activity among important selected agricultural crops. The present paper may also be the starting point of the research aimed for development of new functional food and nutraceuticals, and/or of the research focused on new secondary metabolites with potential as bioactive compounds.Key words: trypsin; thrombin; urokinase; inhibition; antioxidant; activity; forage crops.Abbreviations: AOX, antioxidant activity; BBI, Bowman-Birk inhibitor; DMSO, dimethyl sulphoxide; Rel%BT, relative percentage value of activity to standardized black tea extract; Rel%Q, relative percentage value of activity to standard quercetin; SHE, sunflower oil hydrolysate ethanol; SITh, selectivity index of thrombin; SIU, selectivity index of urokinase; ThIA, thrombin inhibition activity; TrIA, trypsin inhibition activities; UIA, urokinase inhibition activity.

Thermal and acido-basic stability of antioxidant properties of extracts from cereal and pseudocereal grains

“…Nevertheless both genotypes of buckwheats contain more polyphenols and flavonoids also due to a high content of the well-known flavonoid rutin (Jiang et al 2007), a compound typical and specific for this crop with well documented health beneficial effects. Achieved results are in rough correlation with papers published previously (Quettier-Deleu et al 2000;Ivanišová et al 2010) and our achieved results (Maliar et al 2011).…”

Antioxidant and Proteinase Inhibitory Activities of Selected Poppy (Papaver somniferum L.) Genotypes

“…In comparison with the extracts from hop ( Humulus lupulus L.) cones, the poppy grain extracts expressed approximately two times higher activities of IA_TR. On the contrary, IA_TR in alfalfa ( Medicago sativa L.) leaf extracts were the inhibitory activities two times lower. The highest IA_TY was found in the genotypes Redy , MS ZB‐3 and MS 423, while the lowest in the genotype Aristo ( Table ).…”

“…The highest IA_TY was found in the genotypes Redy , MS ZB‐3 and MS 423, while the lowest in the genotype Aristo ( Table ). An average value of IA_TY in poppy seeds was three times higher than in the extracts from hop cones, two times higher in comparison with oat seed extracts and also higher than in leaf extracts from alfalfa . The IA_UR of the 15 poppy genotypes were in the range of 31.0 – 51.8% ( Table ).…”

“…An average value of IA_TY in poppy seeds was three times higher than in the extracts from hop cones, [33] two times higher in comparison with oat seed extracts [35] and also higher than in leaf extracts from alfalfa. [34] The IA_UR of the 15 poppy genotypes were in the range of 31.0 -51.8% ( Table 3). The highest IA_UR was in seed extract from the genotype BUDDHA.…”

Trifolium pratense

“…Additionally, prunetin and pseudobaptigenin were detected in human plasma for the fi rst time. Maliar et al ( 2011 ) found that Trifolium pratense accession POLKIE99-3 expressed the highest relative trypsin inhibition activity (80.0 %) compared to standards. They also found that Trifolium pratense genetic resources SVKZAH98-40 possessed relatively high antioxidant properties.…”