Antioxidant and Proteinase Inhibitory Activities of Selected Poppy (<i>Papaver somniferum</i> L.) Genotypes

Krošlák, Erik; Maliar, Tibor; Nemeček, Peter; Viskupičová, Jana; Maliarová, Mária; Havrlentová, Michaela; Kraic, Ján

doi:10.1002/cbdv.201700176

Cited by 18 publications

(16 citation statements)

References 43 publications

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“…Consequently, many countries forbid or strictly control the cultivation of P. somniferum and the uses of opium products through legislation. However, P. somniferum is attractive for researchers, and research efforts have been undertaken in many aspects, including the pharmacological activity and metabolism of alkaloids in the plant [5,6].…”

Section: Introductionmentioning

confidence: 99%

Selection of the Reference Gene for Expression Normalization in Papaver somniferum L. under Abiotic Stress and Hormone Treatment

Zhang

et al. 2020

Genes

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Papaver somniferum L. is an important medical plant that produces analgesic drugs used for the pain caused by cancers and surgeries. Recent studies have focused on the expression genes involved in analgesic drugs biosynthesis, and the real-time quantitative polymerase chain reaction (RT-qPCR) technique is the main strategy. However, no reference genes have been reported for gene expression normalization in P. somniferum. Herein, nine reference genes (actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), cyclophilin 2 (CYP2), elongation factor 1-alpha (EF-1α), glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), nuclear cap-binding protein subunit 2 (NCBP2), protein phosphatase 2A (PP2A), TIP41-like protein (TIP41), and tubulin beta chain (TUB)) of P. somniferum were selected and analyzed under five different treatments (cold, drought, salt, heavy metal, and hormone stress). Then, BestKeeper, NormFinder, geNorm, and RefFinder were employed to analyze their gene expression stability. The results reveal that NCBP2 is the most stable reference gene under various experimental conditions. The work described here is the first report regarding on reference gene selection in P. somniferum, which could be used for the accurate normalization of the gene expression involved in analgesic drug biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Selection of the Reference Gene for Expression Normalization in Papaver somniferum L. under Abiotic Stress and Hormone Treatment

Zhang

et al. 2020

Genes

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“…The proteinases were selected as the third group of variables (secondary metabolites content, antioxidant activity parameters). As the result, the presented paper represents a continuity of several, yet published papers with identical methodology and alternate plant subjectbarley (Maliar et al 2015), oat (Krošlák et al 2016) and poppy (Krošlák et al 2017), enabling comparison and discussion of obtained results. Further, the selected secondary metabolites are main carriers of antioxidant activity as well as proteinase inhibition activity in the plant tissue (Meijersen et al 1991;Fink et al 2000;Maliar et al 2015;Yu et al 2018).…”

Section: Determination Of Proteinase Inhibition Activity Parametersmentioning

confidence: 88%

Biologically valuable components, antioxidant activity and proteinase inhibition activity of leaf and callus extracts of Salvia sp.

Vulganová¹,

Maliar²,

Maliarová³

et al. 2019

Nova Biotechnologica Et Chimica

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Sage is medicinal plant, known for its antioxidant and anti-inflammatory effects. Eight extract samples were tested in this study: extract from Salvia officinalis L. varieties from two different geographical localities (Jaslovské Bohunice and Pobedim, Slovakia), Salvia officinalis L., variety “bicolor”, Salvia officinalis L., variety “purpurescens”, Salvia apiana, Salvia divinorum, and two callus cultures of Salvia sclarea L. and Salvia aethiopis L. The highest values for composite parameters were observed for extract from Salvia apiana. It can be concluded that prepared sage extract samples are rich on polyphenolic acids (2 950±265 μg.mL−1 GAeq.) and amines (197±5.50 μg.mL−1 TRPeq.). HPLC analysis confirmed the dominant content of rosmarinic acid in the extracts; the highest content was detected in the Salvia apiana extract (1 120±15 μg.mL−1). Extract from Salvia apiana expressed too the highest antioxidant activity (1 710 – 4 669 μg.mL−1TEAC). Similarly, the highest inhibition activity was observed for this extract on thrombin (57±3.3 %) and on other proteinases (over 80 %). Spearman correlation analysis and PCA analyses revealed a coherence between antioxidant activity of samples and their content of rosmarinic acid as well as inhibitory activity towards particular proteases, and revealed the significance of thiol based secondary metabolites. Cluster analysis demonstrates the differences of Salvia apiana extract from extracts of S. officinalis L., the group of S. divinorum extract and from callus cultures.

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“…Antioxidant, anti-inflammatory, immunomodulatory, various proteinase inhibitory activities (Kroslak et al, 2017) . Traditionally, it is used to treat stomach irritation, diarrhea, and abdominal cramp.…”

Section: Curcuminmentioning

confidence: 99%

“…It also contains various minerals such as copper, magnesium, zinc, iron, manganese, and phosphorus. It showed antioxidant and various proteinase inhibitory activities (Kroslak et al, 2017). Furthermore, each poppy seed contains an enormous quantity of zinc, therefore it is believed that it can stimulate the production of white blood cells and increase immunity against foreign agents (Rink & Gabriel, 2000).…”

Section: Immunomodulatory Effects Of Herbs and Spicesmentioning

confidence: 99%

Herbal spices-based therapeutics for diabetic patients with COVID-19 infection: A review

Rajasekar¹,

Vel²

2021

NRFHH

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The COVID-19 pandemic is a highly challenging situation and affects people's lives severely. Nowadays, the original coronavirus gets mutated into different variants and infects all age groups. Elderly people with comorbidities like diabetes are highly susceptible to coronavirus infection due to poor glycemic control, and immunity. The mechanism of COVID-19 infection is not fully understood. However, the scenario of current infection is similar to the history of pandemics that the world faced earlier (i.e.) the persisted hyperglycemia is considered as the major root cause of severe viral infections. The uncontrolled hyperglycemia can produce low-grade inflammation and impaired immunity mediated cytokine storm that can cause multiple organs failure and sudden death in diabetic patients with COVID-19 infection. The available hypoglycemic and antiviral drugs and vaccines are not sufficiently efficient against COVID-19 and also cause several side effects. As the traditional medicines are considered life-saving drugs for various human diseases, the present review has analyzed the herbs-spices with antiviral, antidiabetic, antioxidant, anti-inflammatory, and immunomodulatory properties. The intake of this herbal-spices formulation could exert both therapeutic and prophylactic effects against the COVID-19. However, the proper scientific studies are necessary for the validation of its efficacy and mode of action against COVID-19.

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Antioxidant and Proteinase Inhibitory Activities of Selected Poppy (Papaver somniferum L.) Genotypes

Cited by 18 publications

References 43 publications

Selection of the Reference Gene for Expression Normalization in Papaver somniferum L. under Abiotic Stress and Hormone Treatment

Selection of the Reference Gene for Expression Normalization in Papaver somniferum L. under Abiotic Stress and Hormone Treatment

Biologically valuable components, antioxidant activity and proteinase inhibition activity of leaf and callus extracts of Salvia sp.

Herbal spices-based therapeutics for diabetic patients with COVID-19 infection: A review

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