Proteinaceous Protease Inhibitor from<i>Lawsonia Inermis</i>: Purification, Characterization and Antibacterial Activity

Dabhade, Arvind R.; Patel, Priti; Pati, U.S.

doi:10.1177/1934578x1300801033

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Therefore, proteases are reported as prime targets for the development of antibacterial drugs that are currently being studied to be obtained from plants. Agreeing with this, the exploration of protease inhibitor from plants has been promoted, such as Moringa oleifera leaves, Cassia fistula, and Lawsonia inermis seeds [58][59][60]. Historically, the leaves and bark of T. sureni are usually used to control mites, stink bugs, caterpillars, and aphids because they contain surenin and surenolactone [61].…”

Section: Resultsmentioning

confidence: 99%

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Kusuma

Herawati²,

Ramdhani³

et al. 2022

Int J App Pharm

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Objective: This study was purposed to determine the most effective inhibition among those repellent plants i.e. The leaves of kirinyuh (Chromolaena odorata), kenikir (Cosmos caudatus), bandotan (Ageratum conyzoides), grass teki (Cyperus Cyperus rotundus), lemongrass (Cymbopogon citratus) and suren (Toona sureni) towards S. typhi clinical isolate. Methods: The ethanolic extracts of sixt plants were obtained by maceration method using 70% ethanol. Phytochemical screening was done using the standard methods as described by Farnsworth. The inhibition of the repellent leaves ethanolic extracts to chloramphenicol resistant-S. typhi clinical isolate assayed using the agar diffusion method and statistically analyzed by ANOVA followed by the Duncan test. The most potential plant was further determined by investigating the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) value using the microdilution test. Results: As the result, all ethanolic leaves extracts contain alkaloids, flavonoids, and tannins, except that tannins were not found in C. rotundus and A. conyzoides. However, all extracts had the activity to inhibit the growth of S. typhi. T. sureni leaves extract evidently showed the strongest inhibition with MIC value in the range of 1.5625<x≤3.125 mg/ml and the MBC value in the range of 6.25<x≤12.5 mg/ml. The ratio of MBC/MIC≤4, thus, T. sureni leaf extract may be classified as a strong bactericidal agent. Conclusion: In summary, T. sureni extract leaves achieved the most appreciable value of MIC MBC and considered as the bactericidal agent which has strong potential to be a novel anti-typhoid fever agent.

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Section: Resultsmentioning

confidence: 99%

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Kusuma

Herawati²,

Ramdhani³

et al. 2022

Int J App Pharm

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“…Lawsonia inermis and Simarouba glauca leaf extracts gave trypsin inhibition of 81.7± 1.3% and 65.2 ± 0.2% respectively (Table 1). A Kunitz type trypsin inhibitor was purified and characterized from the seeds of Lawsonia inermis [23] . Santhosh et al, reported antioxidant activity from Simarouba glauca extract [24] .…”

Section: Trypsin Inhibition By Plant Extractsmentioning

confidence: 99%

Toxicity of plant extracts containing trypsin inhibitor to the larvae of Aedes aegypti

Aparna¹,

Parambil²,

George³

et al. 2021

Int. J. Mosq. Res.

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Mosquitoes are vectors of many pathogens and can transmit a range of potentially fatal diseases. Some of these diseases spread via mosquitoes belonging to the genus Aedes include Dengue, Chikungunya, Yellow fever, Zika etc. Conventional methods of mosquito control are not always completely successful. Therefore new strategies need to be adopted for mosquito control. Plant protease inhibitors are promising candidates as they prevent larval growth by inhibiting gut proteases of mosquito larvae. In this study we screened 15 plant extracts containing trypsin inhibitors for their toxicity to the larvae of Aedes aegypti. Leaf extracts from Lawsonia inermis showed the highest mortality with 100% mortality after 48 hours in second, third and in fourth instar larvae. The treatment with extract from Annona muricata leaf led to 100, 96 and 66% mortality after 48 hours in second, third and fourth instar larvae respectively. After Proteinase K treatment of Lawsonia inermis leaf extract, a reduction in the in vitro inhibition of larval gut protease activity from 82 to 6% and a decrease in mortality from 80 to 50% were observed compared to untreated. This revealed that the mortality, at least in part, is due to the presence of a protenaceous plant protease inhibitor in the extract. Expressing the gene coding for such protease inhibitors in micro organisms or algae upon which the mosquito larvae feeds will serve as an alternate method for control of mosquito population.

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“…Many reports are available on the isolation and purification of protease inhibitor from plants [15,16,17]. For example, a trypsin inhibitor from mung bean seeds was extracted and characterised by Sappasith et al through determining the optimal extraction medium and extract time, heat-treatment, ammonium sulphate precipitation and gel filtration on Sephadex G-50 and purified to 13.51-fold with a yield of 30.25% [18].…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Bowman-Birk Trypsin Inhibitor from Soybean

Gu¹,

Song²,

Zhao³

et al. 2014

JFNR

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In this paper, crude extract of a Bowman-Birk trypsin inhibitor from soybean meal was firstly isolated by a combination of alcohol precipitation, thermal denaturation, isoelectric precipitation and acetone precipitation. Then this extract was purified by DE-52 ion exchange and affinity chromatography. The results showed that soybean Bowman-Birk trypsin inhibitor (SBBI) was purified to 50.07-fold with trypsin activity of 822.31 U·mg-1. The purified SBBI gave a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined to be 8837.46Da by MALDI-TOF. N-terminal sequence showed high homology with other serine proteinase inhibitors belonging to the Leguminosae family.

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Proteinaceous Protease Inhibitor fromLawsonia Inermis: Purification, Characterization and Antibacterial Activity

Cited by 8 publications

References 31 publications

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Toxicity of plant extracts containing trypsin inhibitor to the larvae of Aedes aegypti

Purification and Characterization of Bowman-Birk Trypsin Inhibitor from Soybean

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