Proteinaceous Pancreatic Lipase Inhibitor from the Seed of Litchi chinensis

Abstract

A study of the pancreatic lipase inhibitory activity of a protein from the seed of Litchi chinensis was carried out. Protein was isolated by 70 % ammonium sulphate precipitation followed by dialysis. Lipase inhibitory activity of the protein was evaluated using both synthetic (p-nitrophenyl palmitate) and natural (olive oil) substrates. Protein at the final concentration of 100 µg/mL was able to inhibit 68.2 % pancreatic lipase on synthetic substrate and 60.0 % on natural substrate. Proteinaceous nature of the inhibitor was determined using trypsinization assay. Pancreatic lipase inhibitory protein was sensitive to 0.05 % trypsin treatment with the loss of 61.9 % activity. IC50 of this proteinaceous pancreatic lipase inhibitor was 73.1 µg/mL using synthetic substrate. This inhibitory protein was sensitive to pH, with the highest inhibitory activity at pH=8.0 and the lowest at pH=3.0. Protein was further analyzed using 10 % non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and, interestingly, it showed the presence of a single band of (61±2) kDa when stained with Coomassie brilliant blue. The isolated protein was finally crystallized to see its homogeneity by batch crystallization method. Crystals were well formed with distinct edges. The isolated protein showed good pancreatic lipase inhibitory activity.

Antioxidant Properties and Diet-Related α-Glucosidase and Lipase Inhibitory Activities of Yogurt Supplemented with Safflower (Carthamus tinctorius L.) Petal Extract

“…Additionally, hyperlipidemia is a common feature in patients with diabetes mellitus and is one of the factors that is associated with considerably increases the risk of premature atherosclerosis (Schofield et al, 2016). It is known that pancreatic lipase regulates the digestion and absorption of dietary lipids ( Mhatre et al, 2019 ). Therefore, inhibition of this enzyme may help prevent complications by regulating lipid levels in the blood of diabetic patients through reducing dietary fat absorption into the body.…”

Potential Synergistic Action of Liquid Olive Fruit Polyphenol Extract with Aqueous Extracts of Solid Wastes of Pomegranate or/and Orange Juice Industry as Organic Phyto-protective Agents against Important Plant Pathogens - Part 1 (in vitro Studies)

“…Thus, the interest in the isolation, examination and use of plant tissues and agricultural waste rich in natural polyphenols extracted from various parts of plant species such as thyme, citrus and olive green tea has been expressed by many researchers [59][60][61][62][63][64][65][66][67][68][69][70][71][72][73]. This interest is strongly enhanced since several microorganisms such as fungi, bacteria, viruses, etc., interact in the natural environment with plants [74][75][76] and by them shelves [77] affecting growth and development [78,78,79].…”

Potential of enhancing anti-obesogenic agriceuticals by applying sustainable fertilizers during plant cultivation

“…Flavonoid glycosides were the major specialized constituents of such extract (mainly quercetin and kaempferol), with kaempferol-3-O-β-(2 ′′ -O-glucosyl)-rutinoside and kaempferol-3-O-α-arabinosyl-7-O-rhamnoside being the most abundant flavonols; the crude methanolic extract exhibited a potent inhibitory activity, with an IC 50 value of 2.06 ± 0.02 mg/mL. A protein from the Litchi chinensis seed at 100 µg/mL could also inhibit 68.2% of the pancreatic lipase activity, with an IC 50 of 73.1 µg/mL (Mhatre et al, 2019). Bajes et al (2020) described that polysaccharides (e.g., pectin) could reduce fat absorption in the gastrointestinal tract by changing the characteristics of gastrointestinal fluids, restricting pancreatic lipase activity (competitive inhibition), and affecting the fat aggregation state.…”