Protein transmission during Dean vortex microfiltration of yeast suspensions

Kluge, Tanja; Rezende, Carla Ferreira; Wood, David; Belfort, Georges

doi:10.1002/(sici)1097-0290(19991220)65:6<649::aid-bit5>3.0.co;2-d

Cited by 16 publications

(5 citation statements)

References 12 publications

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“…However, numerous studies have shown that the fouling of MF membranes by proteins during filtration can be severe, resulting in low fluxes and protein transmission. [6][7][8][9][10][11] Numerous techniques have been used to characterize the protein fouling of membranes. They were recently thoroughly reviewed by Chan and Chen.…”

Section: Introductionmentioning

confidence: 99%

“…The pores of MF membranes (typically 0.1−1 μm diameter) are much larger than native proteins (typical dimensions of a few nanometers), hence little rejection of proteins would be expected at the surface of the membrane and protein transmission should be high. However, numerous studies have shown that the fouling of MF membranes by proteins during filtration can be severe, resulting in low fluxes and protein transmission. − …”

Section: Introductionmentioning

confidence: 99%

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In Situ Three-Dimensional Characterization of Membrane Fouling by Protein Suspensions Using Multiphoton Microscopy

et al. 2006

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Fouling of microfiltration membranes leads to severe flux declines and the need to clean or replace the membrane. In situ 3D characterization of protein fouling both on the surface and within the pores of the membrane was achieved using multiphoton microscopy. Time-lapse images of the fouled membrane were obtained for single suspensions and mixtures of fluorescently labeled bovine serum albumin and ovalbumin. Deposited protein aggregates were visible on the membrane and evidently play an important role in fouling. A combination of 3D images and resistance versus time data was used to identify the dominant fouling mechanism. Fouling is initially internally dominated, but after 1 and 15 min for ovalbumin and bovine serum albumin, respectively, the fouling becomes externally dominated. This is in good agreement with two-stage protein fouling models.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Situ Three-Dimensional Characterization of Membrane Fouling by Protein Suspensions Using Multiphoton Microscopy

et al. 2006

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“…[1][2][3] Stability analysis of this problem explains this effect in Poiseuilletype flows in curved pipes and channels. 4 At present the Dean vortices are known as a means for intensification of heat 5 and mass 6 transfer in single-phase liquids. Recently, certain attempts were made to intensify mass transfer of a passive scalar through a boundary separating two immiscible liquids by creating spatially periodic vortical flows inside both liquid phases, using a two-fluid Taylor-Couette apparatus.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional instability of a two-layer Dean flow

2001

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Stability of a two-layer Dean flow in a cylindrical annulus with respect to three-dimensional perturbations is studied by a global Galerkin method. It is shown that for large inner radius of the annulus ͑i͒ the instability becomes three-dimensional if one of the fluid layers is thin, ͑ii͒ its onset is not affected by possible small deformations of the interface, and ͑iii͒ multiple three-dimensional flow states are expected in a slightly supercritical flow regime. Stability diagrams and patterns of the three-dimensional perturbations are reported. It is concluded that even when the axisymmetric perturbation is the most dangerous, the resulting supercritical flow is expected to be three-dimensional. Possible multiplicity of supercritical three-dimensional states is predicted. The basis functions of the global Galerkin method are constructed so as to satisfy analytically the boundary conditions on no-slip walls and at the liquid-liquid interface. A modification of the numerical approach, accounting for small deformations of the interface which is subject to the action of the capillary force, is proposed. The results are of potential importance for development of novel bioseparators employing Dean vortices for enhancement of mass transfer of a passive scalar ͑say, a protein͒ through the interface. The developed numerical approach can be used for stability analysis in other two-fluid systems.

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“…However, while the main application of Taylor instability appears to be in relation to Couette viscometers, application of Dean instability appears to be much broader encompassing heat and mass transfer enhancement, chaotic mixing, microfluidics, and microfiltration, among others. [5][6][7][8] The flow is also of fundamental importance in studies related to hydrodynamic instability theories. 9,10) Thus, it is of no surprise that Dean instability has been investigated so extensively in the past, for both Newtonian and non-Newtonian fluids alike.…”

Section: Introductionmentioning

confidence: 99%

Dean Instability of Bingham Fluids in Tangential Flow between Two Fixed Concentric Cylinders

Soleimani

Sadeghy

2010

J. Soc. Rheol., Jpn

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In the present work, hydrodynamic instability of viscoplastic fluids is investigated in azimuthal pressure-driven flow between two fixed concentric cylinders-the so-called Dean flow. To avoid stress discontinuity anywhere in the gap, a regularized Bingham model was used to represent viscoplastic fluids. To determine conditions for the onset of instability, an infinitesimal disturbance was introduced to the basic flow and its evolution in time was checked using a normal-mode linear stability analysis. An eigenvalue problem was obtained which was solved numerically using the pseudo-spectral collocation method. A plot of the Dean number versus the Bingham number showed that the yield stress has always a stabilizing effect on the Dean flow. That is to say that, the critical Dean number is increased the higher the Bingham number. Results obtained at three different gap spacing suggest that by an increase in the gap size the flow becomes progressively more stable, but, only if the Bingham number is sufficiently low. At high enough Bingham numbers, however, it is predicted that by an increase in the gap size, Dean flow may become less stable.

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Protein transmission during Dean vortex microfiltration of yeast suspensions

Cited by 16 publications

References 12 publications

In Situ Three-Dimensional Characterization of Membrane Fouling by Protein Suspensions Using Multiphoton Microscopy

In Situ Three-Dimensional Characterization of Membrane Fouling by Protein Suspensions Using Multiphoton Microscopy

Three-dimensional instability of a two-layer Dean flow

Dean Instability of Bingham Fluids in Tangential Flow between Two Fixed Concentric Cylinders

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