Protein transmission during Dean vortex microfiltration of yeast suspensions

Abstract

Substantially higher rates of protein and fluid volume transport for microfiltration of yeast suspensions were possible with improved hydrodynamics using centrifugal fluid instabilities called Dean vortices. Under constant permeate flux operation with suspended yeast cells, a helical module exhibited 19 times the filtration capacity of a linear module. For feed containing both BSA and beer yeast under constant transmembrane pressure with diafiltration, about twice as much protein (BSA and other proteins from cell lysis) was transported out of the feed by the helical module as compared with the linear module. The volumetric permeation flux improvements for the helical over the linear module ranged from 18 to 43% for yeast concentrations up to 4.5 dry wt %.

Stationary d.c. streaming due to shape oscillations of a droplet and its effect on mass transfer in liquid–liquid systems

“…a protein) at a liquid-liquid interface (Baier & Graham 1998;Baier et al 1999). Secondary Dean vortices arising in flows in curved (helical) pipes have already found application in a novel biotechnological module (Gehlert, Luque & Belfort 1998;Kluge et al 1999;Luque et al 1999). In these applications secondary Dean vortices are used to prevent blockage of permeable tubular membranes.…”

Selective precipitation‐assisted recovery of immunoglobulins from bovine serum using controlled‐fouling crossflow membrane microfiltration

“…Diafiltration was performed in the constant permeate flux mode by employing a pump on the permeate side. Benefits of this technique for reducing fouling have been reported extensively for cell-protein separations (Kluge et al, 1999;Maiorella et al, 1991;van Reis et al, 1991). As seen from Figure 3a and b, 97% of the BSA was removed in 5 diavolumes.…”

Optimized Removal of Soluble Host Cell Proteins for the Recovery of met-Human Growth Hormone Inclusion Bodies from Escherichia coli Cell Lysate Using Crossflow Microfiltration

“…An important technical challenge that needs to be addressed with membrane processes is the reduction of fouling to manageable levels (4). Approaches to this problem include optimizing the solution conditions (4), developing improved fluid mechanics (5, 6), offering protein‐resistant membranes (7−9), and combining membrane filtration with other processes (10).…”

“…An important technical challenge that needs to be addressed with membrane processes is the reduction of fouling to manageable levels (4). Approaches to this problem include optimizing the solution conditions (4), developing improved fluid mechanics (5,6), offering proteinresistant membranes (7)(8)(9), and combining membrane filtration with other processes (10). (13) extended the use of crossflow microfiltration (MF) for the purification of IBs of recombinant interleukin-2 (r-IL 2) by partial solubilization of the insoluble cell debris after removal of the soluble HCP into the permeate.…”