Protein subunits in dormant and germinating soybean seeds

Histochemical Studies on Cotyledon of Soybean during Germination

“…More re cently investigations on the protein bodies in soybean seeds were carried out (Saio and Watanabe 1966, Tombs 1967, Catsimpoolas et al 1968). The present micro scopical histochemical investigation on the changes in total insoluble polysaccha rides, neutral lipids, proteins and ribonucleic acid (RNA) in soybean cotyledons upon germination is undertaken to bridge the gap concerning this aspect and to give a comprehensive totality to the metabolism of these seeds.…”

Biochemistry of black gram(phaseolus mungoL.): A review

“…Fractions I, II, and III were electrophoresed by employing the method of Catsimpoolas et al 5 The mobilities of the protein subunits in all three fractions were slightly different when compared. Changes in protein subunit mobilities observed may be due to the formation and presence of complexes between phytate, protein, and minerals.…”

“…Protein extracts were run on the PAGE (polyacrylamide gel electrophoresis), 12 SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), 74 and PAMU-PAGE (phenol-acetic acid-mercaptoethanol-urea-polyacrylamide gel electrophoresis). 5 Typical electrophoretic patterns of black gram proteins extracted with 5% NaCl are shown in Figure 1. Failure of Davis' procedure to give defined bands was expected for the Figure 1B).…”

A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA

“…5.0-mm inside diameter glass tubes were filled to a height of 7 cm with the acrylamide solution described by Catsimpoolas et al ., 1968 . 35% acetic acid was layered over the gel solution ; the tubes, sealed with Parafilm (American Can Co ., Neenah, Wis .)…”

“…This method provides sharp resolution of many water insoluble proteins on the basis of both charge and molecular weight (Work, 1964 ;Takayama, et al ., 1964 ;Catsimpoolas, et al ., 1968 ;Wood, 1970) . Fig .…”