Histochemical Studies on Cotyledon of Soybean during Germination

Suryanarayana, A.

doi:10.1508/cytologia.41.243

Cited by 3 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations of protein bodies are similar to those reported for other tissues, such as peanut (Bagley et al, 1963), pea (Bain and Mercer, 1966), Yucca (Horner and Amott, 1965) and Phaseolus (Opik, 1966). Suryanarayana (1976), suggests that protein bodies in soybean cotyledons undergo progressive fragmentation into smaller particles and finally disappear from most cotyledon cells by 9 days after germination. In a study by McAlister and Krober (1951), protein decreased at about the same rate as oil for the first two weeks, but protein depletion was much slower thereafter.…”

Section: Germinationsupporting

confidence: 87%

“…Tombs (1967) found that disruption and coalescence of protein bodies generally occurred in the epidermis layer and around the vascular bundles, particularly near the embryonic end. Similarly, Suryanarayana (1976) reported that the size and stainabiliiy of all soybean reserve materials decreased in cells bordering vascular strands compared to those away from them. He suggested that this was partly due to the enzyme stimulating effect of hormones that moved into these cells from the phloem and partly due to the sustained enzyme activity in the cells created by the continual removal of end products of degradation by the phloem transport mechanism.…”

Section: Germinationmentioning

confidence: 85%

“…Tombs (1967), in a report on protein bodies of the soybean, found that lipid attached itself tenaciously to protein bodies in homogenates of whole soybeans, thus suggesting a strong lipid body-protein body interaction. Suryanarayana (1976) found that oil globules in sectioned cotyledon tissue were conspicuously seen only after pretreatment with 50% ethanol or following progressive protein body fragmentation during germination, suggesting a tenacious attachment to the protein body mass. Furthermore, in freeze-fractured cotyledons, protein bodies were more readily seen when section surfaces were first washed with hexane.…”

Section: Lipid Bodiesmentioning

confidence: 94%

“…In day 0 and day 1 germinated soybean cotyledons, Suryanarayana (1976) found that most cells were devoid of starch grains. By day 2 to day 5, however, almost all cells contained them.…”

Section: Germinationmentioning

confidence: 95%

“…In a hislochemical study of soybean cotyledons during germination, Suryanarayana (1976), observed that during early stages of germination, neutral lipid, stained with Nile blue chloride, appeared as a faintly stained viscous mass covering the protein bodies. From day 1 to day 5 there was little qualitative change in the oil globules in most cells, but subsequently from day 5 to day 14 there was a noticeable progressive decrease in oil globule content.…”

Section: Germinationmentioning

confidence: 99%

See 4 more Smart Citations

Microscopy of soybean seeds: cellular and subcellular structure during germination, development and processing with emphasis on lipid bodies

Bair

View full text Add to dashboard Cite

Section: Germinationsupporting

confidence: 87%

Section: Germinationmentioning

confidence: 85%

Section: Lipid Bodiesmentioning

confidence: 94%

“…In day 0 and day 1 germinated soybean cotyledons, Suryanarayana (1976) found that most cells were devoid of starch grains. By day 2 to day 5, however, almost all cells contained them.…”

Section: Germinationmentioning

confidence: 95%

Section: Germinationmentioning

confidence: 99%

See 3 more Smart Citations

Microscopy of soybean seeds: cellular and subcellular structure during germination, development and processing with emphasis on lipid bodies

Bair

View full text Add to dashboard Cite

Spatial activity mapping of ß-mannanase on soybean seeds

Rueckel,

Janson,

Solbak

et al. 2024

Sci Rep

View full text Add to dashboard Cite

For farm animals the supplementation of exogenous enzymes, like ß-mannanase, to soybean-based diets is beneficial to improve feed digestibility. In order to unravel the effect of ß-mannanase on soybean meal’s cell structure, a novel imaging concept was developed which allows visualizing the spatial activity pattern of ß-mannanase with high sensitivity by fluorescence microscopy before any visible degradation of the cellular structure occurs. It is based on fluorescence labeling of newly formed reducing ends of ß-mannanase-hydrolyzed polysaccharides after the native reducing ends of all polysaccharides present were chemically reduced. It was revealed that ß-mannanase is not only active at the cell wall but also at previously unknown sites, like the middle lamella and, most prominently, at an intracellular matrix enclosing the protein storage vacuoles. Based on these findings it can be hypothesized that the evaluated ß-mannanase can degrade the enclosing matrix of encapsulated proteins and the cell wall structure and thereby improves efficiency of feed utilization.

show abstract

Microscopy of soybean seeds: cellular and subcellular structure during germination, development and processing with emphasis on lipid bodies

Bair

View full text Add to dashboard Cite

Soybean Seed Anatomy 4 Subcellular Components 8 Protein bodies 8 Starch granules 13 Calcium oxalate crystals Lipid Bodies 17 Soybeans The controversy 20 Appearance 22 Light microscopy Electron microscopy Membrane Cellular distribution and association Chemical composition Function Cellular origin Germination and Developmental Studies of Soybean Cotyledons Germination Maturation Relationship of Processing and Soybean Ultrastructure Conversion of soybeans to several food products Processing of soybeans to desolventized-toasted flakes Solvent extraction of lipids iii Page MATERIALS AND METHODS 63 Soybeans 63 Processed Soybean Samples 63 Proximate Analysis 63 Moisture determination 63 Protein determination 63 Crude lipid 64 Ash determination 65 Carbohydrate 65 Protein Dispersibilify Index (PDI) Microscopy Studies 67 Light microscopy (LM) Paraffin embedding Resin embedding Transmission electron microscopy (TEM) Scanning electron microscopy (SEM) Energy dispersive X-ray (EDX) analysis Photography: (LM), (TEM) and (SEM) Germination and Maturation Studies Germination Cytochemical studies 71 Acid phosphatase localization 71 Catalase localization 72 Lipase localization 73 Maturation 73 îv Page Processing Methods 74 Solvent extraction of soybean cotyledons 74 Physical treatments 74 Heat treatment (boiling) 75 Freezing-thawing treatment 75 Sonication treatment 75 Enzyme treatment (trypsin) 75 Lipid Body Isolation 75 Density Gradient Centrifugation 76 RESULTS AND DISCUSSION 78 Soybean Cytology with Emphasis on Protein Bodies 78 Starch Granules 97 Calcium Oxalate Crystals Soybean Lipid Bodies Appearance and distribution Light microscopy Electron microscopy Isolation and chemical characterization of lipid bodies Isolation of lipid bodies Chemical composition Effect of isolation medium Effect of soybean cotytedon:isolation medium ratio Effect of centrifugation temperature Effect of heating V Page Microscopic examination of isolated lipid bodies 135 Membrane 143 Effect of trypsin treatment 150 Examination of centrifugation pellet 153 Problems associated with microscopical preparation of 158 isolated lipid bodies Density gradient centrifugation of lipid bodies 158 Germination and Developmental Studies of Soybean Cotyledons 159 Germination Maturation 189 Relationship of Processing and Soybean Ultrastructure 211 Ultrastructural changes in soybeans during processing to yield 211 desolventized-toasted meal Effect of boiling Effect of trypsin treatment Effect of freezing-thawing Effect of solvent extraction Effect of son ication

show abstract

Histochemical Studies on Cotyledon of Soybean during Germination

Cited by 3 publications

References 14 publications

Microscopy of soybean seeds: cellular and subcellular structure during germination, development and processing with emphasis on lipid bodies

Microscopy of soybean seeds: cellular and subcellular structure during germination, development and processing with emphasis on lipid bodies

Spatial activity mapping of ß-mannanase on soybean seeds

Microscopy of soybean seeds: cellular and subcellular structure during germination, development and processing with emphasis on lipid bodies

Contact Info

Product

Resources

About