Protein separation using non-porous sorbents

Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns

“…To avoid slow mass transfer in stagnant pores, which are essential for a large surface area and hence for a high capacity, the pores should be shorter or completely eliminated. One approach was the introduction of non-porous particles (for review see [6]). They exhibit a small surface area and are useful for analytical purposes where there is no need for a high binding capacity.…”

Porous Polymer Monoliths: An Alternative to Classical Beads

“…Separation conditions: 1.25 min gradient of THF in methanol consisting of 0-35% THF in methanol in 0.12 min, 35-50% in 0.38 min, 50-55% in 0.25 min, 55-59% in 0.25 min, and 59-60% in 0.25 min, overall sample concentration 16 mg/ml (2 mg/ml of each standard) in THF, ELSD detection. Molecular weights of polystyrene standards: 3000 (1), 7,000 (2), 12,900 (3), 20,650 (4), 50,400 (5), 96,000 (6), 214,500 (7), and 980,000 (8). Dotted line shows the same separation recorded more than two months and about 400 injections later achieved in only 3 min.…”

“…Separation media consisting of a rigid porous silica matrix with pores filled with a soft hydrogel also exhibit very good mass transfer characteristics [4,5]. The use of non-porous beads made from both silica [6,7] and synthetic polymers [8,9] is the ultimate solution to the problem of diffusion into pores of a separation medium. Non-porous media are becoming very popular for the separation of proteins, oligonucleotides, and DNA fragments.…”

Fast cation-exchange separation of proteins in a plastic microcapillary disc

“…For a conventional porous adsorbent under typical operating conditions the overall mass transfer resistance to adsorption [8] is of relative magnitude 240 × EBC. For MCF's, with no intraparticle diffusion resistance and high film mass transfer rates, the overall mass transfer resistance is only of order 0.023 × EBC.…”