“…Separation conditions: 1.25 min gradient of THF in methanol consisting of 0-35% THF in methanol in 0.12 min, 35-50% in 0.38 min, 50-55% in 0.25 min, 55-59% in 0.25 min, and 59-60% in 0.25 min, overall sample concentration 16 mg/ml (2 mg/ml of each standard) in THF, ELSD detection. Molecular weights of polystyrene standards: 3000 (1), 7,000 (2), 12,900 (3), 20,650 (4), 50,400 (5), 96,000 (6), 214,500 (7), and 980,000 (8). Dotted line shows the same separation recorded more than two months and about 400 injections later achieved in only 3 min.…”