Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns

Monolith-based immobilized metal affinity chromatography increases production efficiency for plasmid DNA purification

“…Table 1 The comparison of the pDNA purification productivity ( ) from alkaline cell lysate using either monolith or particle-based column. Process On the contrary, monolith-based chromatographic supports with large channel size of 700-1000 nm allow full utilization of the ligands on the chromatographic resin even at low feed concentration (with incompact structure of RNA), resulting in concentration-independent dynamic binding capacity for RNA molecules [14,22]. Thus the enhanced process efficiency with the use of monolith column is especially highlighted at low RNA feed concentration.…”

“…In a monolith column, due to high porosity, the pressure drop is low and stationary phase structure is intact even at a high flowrate [14]. Moreover, the separation and purification process based on convective flow can result in flow-independent resolution and dynamic binding capacity even for macromolecules [15,16].…”

Large-scale methacrylate monolithic columns: design and properties

“…Passage of the mobile phase through the monolith is due to the geometry, accompanied with the flux increase and, consequently, increases of the linear velocity [11], which in the case of conventional porous particulate supports results in decreased resolution [15]. Since monoliths exhibit flow-unaffected characteristics [4,5] no such effects were observed and the resolution was not influenced in the tested range [10]. From the end user's point of view, there is no difference in the performance between axial or radial monolithic columns and very fast separations are also obtained with the large columns (Fig.…”

Short columns with molecularly imprinted monolithic stationary phases for rapid separation of diastereomers and enantiomers

“…With respect to conventional stationary phases, flow rate has very large influence on the dynamic binding capacity because diffusion limits the adsorption of analytes on the binding sites with the increase of flow rate, as a result the dynamic capacity is compromised at elevated flow rate [31]. However, in the case of monolithic supports, convection becomes a dominant transport mechanism [9], as a consequence the dynamic binding capacity is largely independent of the flow rate, particularly at high flow rates [32]. The effect of flow rate on the dynamic binding capacity of cinchonine was investigated, and the obtained results were shown in Fig.…”