Protein-RNA interaction guided chemical modification of Dicer substrate RNA nanostructures for superior in vivo gene silencing

Jang, Bora; Jang, Hye-Jin; Kim, Hyun‐Sook; Kim, Minjeong; Jeong, Michaela; Lee, Gyeong Seok; Lee, Kyuri; Lee, Hyukjin

doi:10.1016/j.jconrel.2021.11.009

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…[143][144][145] On the other hand, IVT mRNA has been also co-delivered with its adjuvants to achieve strong immune effects with effective antigen recognition or enzymatic degradation resistance. [146][147][148][149][150][151] Here, adjuvants that are simultaneously encapsulated in LNP refer to pathogens-derived biomaterials such as their nucleic acids, or proteins. These adjuvants can help induce antigen-specific T cell and B cell responses.…”

Section: Mrna With Chemical Modificationsmentioning

confidence: 99%

High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications

Seo

Jeon

Kwon

et al. 2023

Adv Healthcare Materials

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The recent development of RNA-based therapeutics in delivering nucleic acids for gene editing and regulating protein translation has led to the effective treatment of various diseases including cancer, inflammatory and genetic disorder, as well as infectious diseases. Among these, lipid nanoparticles (LNP) have emerged as a promising platform for RNA delivery and have shed light by resolving the inherent instability issues of naked RNA and thereby enhancing the therapeutic potency. These LNP consisting of ionizable lipid, helper lipid, cholesterol, and poly(ethylene glycol)-anchored lipid can stably enclose RNA and help them release into the cells' cytosol. Herein, the significant progress made in LNP research starting from the LNP constituents, formulation, and their diverse applications is summarized first. Moreover, the microfluidic methodologies which allow precise assembly of these newly developed constituents to achieve LNP with controllable composition and size, high encapsulation efficiency as well as scalable production are highlighted. Furthermore, a short discussion on current challenges as well as an outlook will be given on emerging approaches to resolving these issues.

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Section: Mrna With Chemical Modificationsmentioning

confidence: 99%

High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications

Seo

Jeon

Kwon

et al. 2023

Adv Healthcare Materials

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“…Methylation is one of the common methods for RNA posttranscription modification. It participates in the regulation of various RNA biological processes in cells, including alternative splicing [ 9 ], maintaining RNA stability [ 10 ] and normal structure [ 11 ], protein translation [ 12 ], and RNA-protein interactions [ 13 ]. Posttranscriptional modifications are installed or removed by enzymatic reactions at specific sites by methyltransferases (writers) and demethyltransferases (erasers), and methylated binding proteins (readers) can read the modified information and act as delivery messengers for the execution of downstream functions [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

5-Methylcytosine-Related Long Noncoding RNAs Are Potential Biomarkers to Predict Overall Survival and Regulate Tumor-Immune Environment in Patients with Bladder Cancer

Wang

Chen

et al. 2022

Disease Markers

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The role of 5-methylcytosine-related long noncoding RNAs (m5C-lncRNAs) in bladder cancer (BLCA) remains unclear. Here, we aim to study the prognostic value, gene expression characteristics, and correlation between the m5C-lncRNA risk model and the tumor microenvironment, immune infiltration, and tumor mutations in BLCA. After collecting BLCA patient RNA sequence transcriptome data, clinical information and mutation data from the Cancer Genome Atlas (TCGA) database, 17 m5C-related lncRNAs independently correlated with OS were obtained by Lasso and multivariate Cox regression analysis, and a risk model was constructed. Univariate Cox, multivariate Cox regression analysis, and the C-index curve proved that the risk model was a significant independent prognostic indicator for patients with BLCA. ESTIMATE and CIBERSORT indicated that the higher the number of immune cells and stromal cells in TME, the higher the prognostic risk. We found that in the low-risk group, the expression levels of immune cells that predicted a good prognosis were higher, including plasma cells, regulatory T cells, and CD 8 T cells. There is a negative correlation between TMB and risk score. The TMB of the low-risk group is significantly higher than that of the high-risk group. In conclusion, the m5C-related risk model is crucial to predict the prognosis of patients with BLCA.

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Oligonucleotide therapeutics and their chemical modification strategies for clinical applications

Kim,

Lee

et al. 2024

J. Pharm. Investig.

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Protein-RNA interaction guided chemical modification of Dicer substrate RNA nanostructures for superior in vivo gene silencing

Cited by 3 publications

References 53 publications

High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications

High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications

5-Methylcytosine-Related Long Noncoding RNAs Are Potential Biomarkers to Predict Overall Survival and Regulate Tumor-Immune Environment in Patients with Bladder Cancer

Oligonucleotide therapeutics and their chemical modification strategies for clinical applications

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