HOWARTH, R. E. 1972. RNA content and protein synthesis in skeletal muscles of young rats: effects of protein deficiency. Can. J. Pkysiol. Phar~nacol. 50,[59][60][61][62][63][64] A protein-free diet was fed to weanling rats for 1, 2, 3, 4, or 7 days before sacrifice in order to determine the time course of RNA depletion from their gastroca~ernius muscles. A 34(% Boss of total RNA occurred on the first 2 days and thereafter depletion occurred at approximately 8% per day.The time course of RNA repletion was determined by refeeding a stock diet to protein-starved rats. A significant increase in total RNA content occurred on the 2nd day and repletion of total RNA content was complete on the 4th day of refeding the stock diet. Rates of muscle protein synthesis in vivo were estimated by 14C incorporation from L-leucine into muscle proteins, and correlations between RNA concentrations and rate of protein synthesis were evaluated by linear regression anlysis. There was no significant relationship between soluble RNA concentration and protein synthesis. The relationship between protein synthesis and microsomal RNA concentration was significant. The resulks skipport the conclusion that availability of ribosomal WNA is a major factor in the long-term regulation of muscle protein biosyntkesis.HOWARTH, R. E. 1972. RNA content and protein synthesis in skeletal muscles of youiag rats: efkcts of protein deficiency. Can. J. Pkysiol. Pharmacol. 50. 59-64.Avant d'etse sacrifit5, des jeunes rats ont ete nourris pendant 1, 2, 3,4, OLI 7 Q O I~~S d'un sCgirne sans psoteines afin de determiner 1e laps dc temps rcquis avant que les m. gwsfrocrtemii soient Cpuises de AWN. Une perte de 34% du total ARN a etC observe aprks les deux premiers jours et par la suite environ 8% d7@puisement est survenue par jour. La periode de temps requise pour restorer B e ARN a Ct C dkterrninee en nourissant des rats privCs de protkines d'un rCyime rkgulier. Un gain significatif en contenu total d'ARN a Ct C not6 le deuxiitme jour et la restoration totale du sontenu AWN etait complete le quatrikmc jour du rkgirne. Ee rnontant de protCines musculaires synthCtisCs in vivo ont Ct C estime en incorporant 14C de L-leucine dans les proteines muscuPaires et %a relation entre Ies concentrations ARN et Ie montant de protkl'nes synthCtisCs ont etC 6valuCs par une analyse de rkgression linkaire. I1 n9existait pas de relation significative entre la concentration AWN soluble et la synthkse protkine. La relation entre la synthkse protkine et la concentration ARN microsomique Ctctit significative. Les resultats s~rpporte la conclusion que le rnontant de ARN ribosomique est un facteklr nnajeur dans la rkg~rlation dc langue durCe de biosynthese de muscle protkine.