Protein regulator of cytokinesis 1 overexpression predicts biochemical recurrence in men with prostate cancer

Luo, Hongwei; Chen, Qing-Biao; Wan, Yuqing; Chen, Guanxing; Zhuo, Yangjia; Cai, Zhiduan; Luo, Zheng; Han, Zhengxue; Liang, Yu‐Xiang; Zhong, Weide

doi:10.1016/j.biopha.2016.01.004

Cited by 24 publications

(19 citation statements)

References 18 publications

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“…For example, the study of Wang et al has revealed that lncRNA HCP5 may promote the malignant behavior of ovarian cancer cells by miR-525-5p/PRC1 crosstalk and Wnt/β-catenin pathway [60]. PRC1 has been associated with many malignant carcinomas, such as NPC [61], prostate cancer [62], and lung cancer [63]. In vitro experiments have shown that PRC1 depletion inhibits the multiplication and invasiveness of NPC, while in vivo studies have found that PRC1 inhibits the neoplasia and radioresistance of NPC [61].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Huang

et al. 2020

Cancer Cell Int

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Background: Nasopharyngeal carcinoma (NPC) is an epithelial malignancy with high morbidity rates in the east and southeast Asia. The molecular mechanisms of NPC remain largely unknown. We explored the pathogenesis, potential biomarkers, and prognostic indicators of NPC. Methods: We analyzed mRNAs, long non-coding RNAs (lncRNAs), and microRNAs (miRNAs) in the whole transcriptome sequencing dataset of our hospital (five normal tissues vs. five NPC tissues) and six microarray datasets (62 normal tissues vs. 334 NPC tissues) downloaded from the Gene Expression Omnibus (GSE12452, GSE13597, GSE95166, GSE126683, and GSE70970, GSE43039). Differential expression analyses, gene ontology (GO) enrichment, kyoto encyclopedia of genes and genomes (KEGG) analysis, and gene set enrichment analysis (GSEA) were conducted. The lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) networks were constructed using the miRanda and Tar-getScan database, and a protein-protein interaction (PPI) network of differentially expressed genes (DEGs) was built using Search Tool for the Retrieval of Interacting Genes (STRING) software. Hub genes were identified using Molecular Complex Detection (MCODE), NetworkAnalyzer, and CytoHubba. Results: We identified 61 mRNAs, 14miRNAs, and 10 lncRNAs as shared DEGs related to NPC in seven datasets. Changes in NPC were enriched in the chromosomal region, sister chromatid segregation, and nuclear chromosome segregation. GSEA indicated that the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 OH kinase/protein kinase B (PI3K-Akt) pathway, apoptotic pathway, and tumor necrosis factor (TNF) were involved in the initiation and development of NPC. Finally, 20 hub genes were screened out via the PPI network. Conclusions: Several DEGs and their biological processes, pathways, and interrelations were found in our current study by bioinformatics analyses. Our findings may offer insights into the biological mechanisms underlying NPC and identify potential therapeutic targets for NPC.

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Huang

et al. 2020

Cancer Cell Int

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“…Numbers studies have reported the role of PRC1 in cancer development. Luo et al found that PRC1 expression levels were significantly higher in prostate cancer (PCa) tissues than those in normal tissues; in addition, overexpression of PRC1 was significantly associated with advanced clinicopathologic features and a shorter biochemical recurrence-free survival in patients with PCa [ 29 ]. Bu et al observed that the mRNA and protein expression levels were up-regulated in high-grade serous ovarian carcinoma (HGSOC) tissues, and knockdown of PRC1 could reduce the proliferation, metastasis, and in vitro multidrug resistance of ovarian cancer cell [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…The deregulation of PRC1 contributes to tumorigenesis and cancer process [ 14 , 15 ]. A recent study confirmed that the dysregulation of PRC1 might be responsible for biochemical recurrence in patients with prostate cancer, implying that PRC1 could be considered as a prognostic indicator [ 16 ]. Consistently, a previous report revealed PRC1 was accumulated in the breast cancer, and PRC1 promoter exhibited cancer-specific activity [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Consistently, a previous report revealed PRC1 was accumulated in the breast cancer, and PRC1 promoter exhibited cancer-specific activity [ 17 ]. Besides, evidence suggests that PRC1 is involved in breast tumorigenesis, and it is a promising target for clinical treatment of breast cancer [ 16 ]. However, it is still unclear whether PRC1 shows similar expression profile and what roles PRC1 exactly plays in colon cancer.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of protein regulator of cytokinesis 1 facilitates tumor growth and indicates unfavorable prognosis of patients with colon cancer

Wang

Xing

et al. 2020

Cancer Cell Int

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Background Protein regulator of cytokinesis 1 (PRC1) has been reported to play important role in the pathogenesis of various cancers. However, its role in colon cancer has not been studied. Here, we aimed to investigate the biological functions and potential mechanism of PRC1 in colon cancer. Methods The expression level of PRC1 in colon cancer tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemical (IHC) staining of a tissue microarray (TMA). Furthermore, colon cancer cell lines HCT116 and SW480 were treated with short hairpin RNAs against PRC1. The biological function of PRC1 was determined by MTT proliferation, colony formation assay, cell cycle, and apoptosis assays. Then, an in vivo tumor formation assay was conducted to explore the effects of PRC1 on tumor growth. Results The mRNA and protein expression levels of PRC1 were highly expressed in colon cancer tissues and cell lines. PRC1 expression was associated with clinicopathological characteristics and overall survival of patients with colon cancer. Knockdown of PRC1 could decrease proliferation and colony forming ability of colon cancer cells, as well as arrested more cells at G2/M phase and promoted cell apoptosis. In cancer cells, the expression pattern of protein regulators included in cell cycle and apoptosis progress were reverted by PRC1 down-regulation. Additionally, PRC1 down-regulation could suppress colon tumor growth and differentiation. Conclusions We confirmed that PRC1 was overexpressed in colon cancer and was associated with poor prognosis of colon cancer patients. PRC1 down-regulation could arrest cell cycle at G2/M stage, inhibit proliferation, and elicit apoptosis. These findings showed the potential of PRC1 to be used for therapeutic approaches in colon cancer.

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“…For example, PRC1 levels signi cantly decrease after cells exit mitosis and enter G1, and are high during the S and G2/M stages [58]. PRC1 has been associated with many malignant carcinomas, such as NPC [59], prostate cancer [60], and lung cancer [61]. In vitro experiments have shown that PRC1 depletion inhibits the multiplication and invasiveness of NPC, while in vivo studies have found that PRC1 inhibits the neoplasia and radioresistance of NPC [59].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Huang

et al. 2020

Preprint

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Background Nasopharyngeal carcinoma (NPC) is an epithelial malignancy with high morbidity rates in the east and southeast Asia. The molecular mechanisms of NPC remain largely unknown. We explored the pathogenesis, potential biomarkers, and prognostic indicators of NPC. Methods We analyzed mRNAs, long non-coding RNAs (lncRNAs), and microRNAs (miRNAs) in the whole transcriptome sequencing dataset of our hospital (five normal tissues vs. five NPC tissues) and six microarray datasets (62 normal tissues vs. 334 NPC tissues) downloaded from the Gene Expression Omnibus (GSE12452, GSE13597, GSE95166, GSE126683, and GSE70970, GSE43039). Differential expression analyses, gene ontology (GO) enrichment, kyoto encyclopedia of genes and genomes (KEGG) analysis, and gene set enrichment analysis (GSEA) were conducted. The lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) networks were constructed using the miRanda and TargetScan database, and a protein-protein interaction (PPI) network of differentially expressed genes (DEGs) was built using Search Tool for the Retrieval of Interacting Genes (STRING) software. Hub genes were identified using Molecular Complex Detection (MCODE), NetworkAnalyzer, and CytoHubba. Results We identified 61 mRNAs, 14miRNAs, and 10 lncRNAs as DEGs related to NPC. Changes in NPC were enriched in the chromosomal region, sister chromatid segregation, and nuclear chromosome segregation. GSEA indicated that the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 OH kinase/protein kinase B (PI3K-Akt) pathway, apoptotic pathway, and tumor necrosis factor (TNF) were involved in the initiation and development of NPC. Finally, 20 hub genes were screened out via the PPI network. Conclusions Our findings could offer insights into the biological mechanisms underlying NPC and identify potential therapeutic targets, biomarkers and prognostic indicators for NPC.

show abstract

Protein regulator of cytokinesis 1 overexpression predicts biochemical recurrence in men with prostate cancer

Cited by 24 publications

References 18 publications

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Overexpression of protein regulator of cytokinesis 1 facilitates tumor growth and indicates unfavorable prognosis of patients with colon cancer

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

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Protein regulator of cytokinesis 1 overexpression predicts biochemical recurrence in men with prostate cancer

Cited by 24 publications

References 18 publications

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

Overexpression of protein regulator of cytokinesis 1 facilitates tumor growth and indicates unfavorable prognosis of patients with colon cancer

­ Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis

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Comprehensive analysis of key genes associated with ceRNA networks in nasopharyngeal carcinoma based on bioinformatics analysis