Overexpression of protein regulator of cytokinesis 1 facilitates tumor growth and indicates unfavorable prognosis of patients with colon cancer

Abstract

Background Protein regulator of cytokinesis 1 (PRC1) has been reported to play important role in the pathogenesis of various cancers. However, its role in colon cancer has not been studied. Here, we aimed to investigate the biological functions and potential mechanism of PRC1 in colon cancer. Methods The expression level of PRC1 in colon cancer tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemical (IHC) staining of a tissue microarray (TMA). Furthermore, colon cancer cell lines HCT116 and SW480 were treated with short hairpin RNAs against PRC1. The biological function of PRC1 was determined by MTT proliferation, colony formation assay, cell cycle, and apoptosis assays. Then, an in vivo tumor formation assay was conducted to explore the effects of PRC1 on tumor growth. Results The mRNA and protein expression levels of PRC1 were highly expressed in colon cancer tissues and cell lines. PRC1 expression was associated with clinicopathological characteristics and overall survival of patients with colon cancer. Knockdown of PRC1 could decrease proliferation and colony forming ability of colon cancer cells, as well as arrested more cells at G2/M phase and promoted cell apoptosis. In cancer cells, the expression pattern of protein regulators included in cell cycle and apoptosis progress were reverted by PRC1 down-regulation. Additionally, PRC1 down-regulation could suppress colon tumor growth and differentiation. Conclusions We confirmed that PRC1 was overexpressed in colon cancer and was associated with poor prognosis of colon cancer patients. PRC1 down-regulation could arrest cell cycle at G2/M stage, inhibit proliferation, and elicit apoptosis. These findings showed the potential of PRC1 to be used for therapeutic approaches in colon cancer.

A pan-cancer analysis of the oncogenic role of protein regulator of cytokinesis 1(PRC1) in human tumors

“…Jia et al con rmed that PRC1 is highly expressed in bladder cancer, and They reported that miR-139-5p derived from exosomes (hUCMSCs) could upstream regulate PRC1 expression, thereby affecting tumor invasion and metastasis 23 . Furthermore, it also has been reported that PRC1 could accelerates cell cycle, inhibits tumor cell apoptosis, and correlates with poor prognosis in Colon cancer 24 . As for ovarian cancer, Bu et al reported that forkhead box protein M1 (FOXM1) can regulated the mRNA and protein expression of PRC1, they found that PRC1 is highly expressed in ovarian cancer and is associated with sensitivity to platinumbased chemotherapy, which predicts poor prognosis 25 .…”

CircKDM1B promotes hepatocellular carcinoma progression through regulating miR‐1322/PRC1 axis

“…Protein regulator of cytokinesis 1 (PRC1), localized on chromosome 15q26.1, is strongly related to cell movement and mitosis 27 . Current studies have also shown that PRC1 is up‐regulated in various tumors such as ovarian cancer, esophageal cancer and colon cancer, and exerts pro‐cancer effect 28–30 . As for HCC, Chen et al identified that PRC1 could promote HCC metastasis and recurrence 31 .…”

“…27 Current studies have also shown that PRC1 is up-regulated in various tumors such as ovarian cancer, esophageal cancer and colon cancer, and exerts pro-cancer effect. [28][29][30] As for HCC, Chen et al identified that PRC1 could promote HCC metastasis and recurrence. 31 In our…”

Identification and Validation of Potential Candidate Genes of Colorectal Cancer in Response to Fusobacterium nucleatum Infection

“…We further searched the literature in PubMed for associations among the 10 hub genes in CRC. Recent studies revealed that upregulated CDK1 promotes CRC cell proliferation via the inhibition of the p53 pathway ( Gan et al, 2017 ), CCNB1 overexpression exerts oncogenic role in CRC cells by phosphorylating CDK1 ( Fang et al, 2014 ), high expression of MAD2L1 drives aneuploidy and carcinogenesis in CRC ( Ding et al, 2020 ), TPX2 promotes proliferation and tumorigenicity of colon cancer cells ( Wei et al, 2013 ), MELK overexpression is significantly correlated with advanced tumor stage and further lymph node metastasis ( Gong et al, 2018 ), TRIP13 and KIF4Acould promote CRC cell proliferation, invasion and migration and subcutaneous tumor formation ( Hou et al, 2018 ; Sheng et al, 2018 ), overexpression of PRC1 and ANLN facilitate CRC tumor growth and proliferation ( Wang et al, 2016 ; Xu et al, 2020 ). The increase expression of these hub genes is closely related to the occurrence and development of CRC.…”