Protein Profiling of Microdissected Pancreas Carcinoma and Identification of HSP27 as a Potential Serum Marker

Melle, Christian; Ernst, Günther; Escher, Niko; Hartmann, Daniel; Schimmel, Bettina; Bleul, Annett; Thieme, Heike; Kaufmann, Roland; Felix, Klaus; Frieß, Helmut; Settmacher, Utz; Hommann, Merten; Richter, Konrad Klaus; Daffner, Wolfgang; Täubig, Horst; Mussack, Thomas; Claussen, Uwe; Eggeling, Ferdinand von

doi:10.1373/clinchem.2006.079194

Cited by 92 publications

(69 citation statements)

References 29 publications

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“…Circulating sHsp27 levels were significantly higher in patients with HCC than in healthy control subjects. Previous studies have reported elevated sHsp27 levels in patients with breast cancer (Fanelli et al 1998;Banerjee et al 2011), non-small cell lung cancer (Zimmermann et al 2012) and both pancreatic and gastric adenocarcinoma (Melle et al 2007;Huang et al 2010); however, this is the first report of a rise in circulating sHsp27 levels in patients with HCC.…”

Section: Discussionmentioning

confidence: 54%

Serum heat shock protein 27 levels in patients with hepatocellular carcinoma

Gruden

Carucci

Lolli

et al. 2013

Cell Stress and Chaperones

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Levels of serum heat shock protein 27 (sHsp27) have been studied in numerous cancer types, but their potential relevance in patients with hepatocellular carcinoma (HCC) is undetermined. Our aim was to compare sHsp27 levels in patients with HCC and HCC-free controls. Specifically, we recruited 71 patients with HCC (80 % with early tumour), 80 patients with chronic liver disease (59 with liver cirrhosis and 21 with chronic active hepatitis) and 42 healthy subjects. sHsp27 was measured by immunoenzymatic assay. Results showed that sHsp27 levels were significantly (p<0.001) higher in patients with HCC than in the other groups, particularly in those with hepatitis C virus (HCV)-related disease. In HCC patients, sHsp27 levels were not associated with prognostic risk factors, such as size/ multiplicity of nodules and stage. In logistic regression analysis, performed in patients with liver disease, logsHsp27 was associated with a significant age-adjusted 2.5-fold increased odds ratio of HCC and with a significant 4.4-fold higher odds ratio of HCC in the subgroup with HCVrelated liver disease. In receiver operating characteristic curve analysis, sensitivity and specificity of the best sHsp27 cut-off value (456.5 pg/ml) for differentiating patients with HCC from those with HCC-free chronic liver disease were 70 and 73 %, respectively. In conclusion, sHsp27 levels are enhanced in patients with HCC and may represent a candidate biomarker of HCC.

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Section: Discussionmentioning

confidence: 54%

Serum heat shock protein 27 levels in patients with hepatocellular carcinoma

Gruden

Carucci

Lolli

et al. 2013

Cell Stress and Chaperones

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“…Besides these main chaperone functions, HSP27 has been associated with apoptosis and cell proliferation and differentiation (6,7). Additionally, HSP27 is over-expressed in many kinds of cancers, although its role in cancer remains unclear (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma

Huang¹,

Ye²,

Huang

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: In a previous study, we found that heat shock protein 27 (HSP27) was over-expressed in gastric adenocarcinoma (GA) tissue. In this study, our goal was to further verify the expression profile of HSP27 in patients with GA. Methods: Western blot and immunohistochemistry were employed to determine HSP27 expression in 50 paired tumor and adjacent normal tissue. ELISA was used to quantify serum HSP27 concentrations in the same 50 GA patients and 50 healthy individuals. Results: Compared to adjacent normal tissues, HSP27 was over-expressed in 25 (50%, ps0.000) and 24 (48%, ps 0.000) cases of GA tissue by Western blot and immunohistochemistry, respectively. ELISA revealed significantly higher serum concentrations of HSP27 in patients with GA patients (means986 pg/mL) compared to healthy individuals (means573 pg/mL) (ps0.003). In addition, infection with Helicobacter pylori (HP) in healthy individuals was associated with increased expression of HSP27 in both gastric mucosa and serum. Conclusions: These data suggest that HSP27 is overexpressed in GA tissue and serum concentrations of HSP27 are increased in patients with GA. Over-expression of HSP27 may indicate a gastric malignant/infectious process. The detection of serum HSP27 concentrations by ELISA may be useful for screening for GA. Clin Chem Lab Med 2010;48:263-9.

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“…63) DJ-1 is translocated from the cytoplasm to nucleus upon addition of a mitogen to the culture medium 26) and is translocated to mitochondria after oxidative stress. 64) Increased levels of DJ-1 expression have been observed in various cancer cells and tissues, including breast, 51,52) prostate, 65,66) lung, 67,68) and endometrial, 69,70) thyroid, 71,72) pancreas, [73][74][75][76] esophageal, 77) ovary, 78,79) cervical, 80) liver, 81) gastric, 82 57,90) and leukemia. 91,92) Secretion of DJ-1 into the serum or nipple fluid has been found in patients with breast cancer.…”

Section: Tumor Suppressor Mm-1 and Neurodegenerationmentioning

confidence: 99%

Common Mechanisms of Onset of Cancer and Neurodegenerative Diseases

Ariga

2015

Biological & Pharmaceutical Bulletin

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Onset of cancer and neurodegenerative disease occurs by abnormal cell growth and neuronal cell death, respectively, and the number of patients with both diseases has been increasing in parallel with an increase in mean lifetime, especially in developed countries. Although both diseases are sporadic, about 10% of the diseases are genetically inherited, and analyses of such familial forms of gene products have contributed to an understanding of the molecular mechanisms underlying the onset and pathogenesis of these diseases. I have been working on c-myc, a protooncogene, for a long time and identified various c-Myc-binding proteins that play roles in c-Myc-derived tumorigenesis. Among these proteins, some proteins have been found to be also responsible for the onset of neurodegenerative diseases, including Parkinson's disease, retinitis pigmentosa and cerebellar atrophy. In this review, I summarize our findings indicating the common mechanisms of onset between cancer and neurodegenerative diseases, with a focus on genes such as DJ-1 and Myc-Modulator 1 (MM-1) and signaling pathways that contribute to the onset and pathogenesis of cancer and neurodegenerative diseases.

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Protein Profiling of Microdissected Pancreas Carcinoma and Identification of HSP27 as a Potential Serum Marker

Cited by 92 publications

References 29 publications

Serum heat shock protein 27 levels in patients with hepatocellular carcinoma

Serum heat shock protein 27 levels in patients with hepatocellular carcinoma

Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma

Common Mechanisms of Onset of Cancer and Neurodegenerative Diseases

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