Serum heat shock protein 27 levels in patients with hepatocellular carcinoma

Gruden, Gabriella; Carucci, P.; Lolli, Valentina; Cosso, Loretta; Dellavalle, E; Rolle, Emanuela; Cantamessa, A.; Pinach, Silvia; Abate, Maria Lorena; Campra, D; Brunello, F.; Bruno, Graziella; Rizzetto, Mario; Perin, Paolo Cavallo

doi:10.1007/s12192-012-0377-8

Cited by 27 publications

(23 citation statements)

References 22 publications

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“…Likewise, the HCC biomarker cystatin C, an inhibitor of cystein proteinases, showed significantly higher levels also in sera from lung cancer patients [132], and the HCC and lung cancer putative biomarker alpha-1 acid glycoprotein 1, an acute phase protein, was found as well elevated in sera and tumor tissues from patients affected by gastric carcinoma [133]. The here described liver cancer biomarker heat shock protein 27, a protein with cytoprotective and anti-apoptotic activity, measured by immunoenzymatic assay, was confirmed to be elevated in an independent cohort of sera from HCC patients [134]. …”

Section: Discussionmentioning

confidence: 96%

Serum Biomarkers Identification by Mass Spectrometry in High-Mortality Tumors

Tessitore

Gaggiano

Cicciarelli

et al. 2013

International Journal of Proteomics

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Cancer affects millions of people worldwide. Tumor mortality is substantially due to diagnosis at stages that are too late for therapies to be effective. Advances in screening methods have improved the early diagnosis, prognosis, and survival for some cancers. Several validated biomarkers are currently used to diagnose and monitor the progression of cancer, but none of them shows adequate specificity, sensitivity, and predictive value for population screening. So, there is an urgent need to isolate novel sensitive, specific biomarkers to detect the disease early and improve prognosis, especially in high-mortality tumors. Proteomic techniques are powerful tools to help in diagnosis and monitoring of treatment and progression of the disease. During the last decade, mass spectrometry has assumed a key role in most of the proteomic analyses that are focused on identifying cancer biomarkers in human serum, making it possible to identify and characterize at the molecular level many proteins or peptides differentially expressed. In this paper we summarize the results of mass spectrometry serum profiling and biomarker identification in high mortality tumors, such as ovarian, liver, lung, and pancreatic cancer.

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Section: Discussionmentioning

confidence: 96%

Serum Biomarkers Identification by Mass Spectrometry in High-Mortality Tumors

Tessitore

Gaggiano

Cicciarelli

et al. 2013

International Journal of Proteomics

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“…Augmentation of Hsp27 level has been observed both in serum [61] and liver tumor tissue [62,63] from patients with HCC and also in tumor and bile from patients with CCA [64]. Increased MK2 and Hsp27 expression in liver cancer tissues may be linked to a higher activity of MK2/Hsp27 signaling pathway.…”

Section: Discussionmentioning

confidence: 97%

Mitogen-activated protein kinase-activated protein kinase 2 mediates resistance to hydrogen peroxide-induced oxidative stress in human hepatobiliary cancer cells

Ho-Bouldoires

Clapéron

Mergey

et al. 2015

Free Radical Biology and Medicine

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The development and progression of liver cancer are characterized by increased levels of reactive oxygen species (ROS). ROS-induced oxidative stress impairs cell proliferation and ultimately leads to cell death. Although liver cancer cells are especially resistant to oxidative stress, mechanisms of such resistance remain understudied. We identified the MAPK-activated protein kinase 2 (MK2)/heat shock protein 27 (Hsp27) signaling pathway mediating defenses against oxidative stress. In addition to MK2 and Hsp27 overexpression in primary liver tumors compared to adjacent nontumorous tissues, the MK2/Hsp27 pathway is activated by hydrogen peroxide-induced oxidative stress in hepatobiliary cancer cells. MK2 inactivation or inhibition of MK2 or Hsp27 expression increases caspase-3 and PARP cleavage and DNA breaks and therefore cell death. Interestingly, MK2/Hsp27 inhibition decreases antioxidant defenses such as heme oxygenase 1 through downregulation of the transcription factor nuclear factor erythroid-derived 2-like 2. Moreover, MK2/Hsp27 inhibition decreases both phosphorylation of epidermal growth factor receptor (EGFR) and expression of its ligand, heparin-binding EGF-like growth factor. A new identified partner of MK2, the scaffold PDZ protein EBP50, could facilitate these effects through MK2/Hsp27 pathway regulation. These findings demonstrate that the MK2/Hsp27 pathway actively participates in resistance to oxidative stress and may contribute to liver cancer progression.

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“…At a serum of 557 ng/mL, OPN has sensitivity, specificity and AUC of 26%, 92.5% and 51%, respectively, for the diagnosis of HCC [55,56] . of 0.749 [60] .…”

Section: Osteopontinmentioning

confidence: 98%

Role of biomarkers in the prediction and diagnosis of hepatocellular carcinoma

Khattab

Fouad

Ahmed

2015

WJH

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The prevalence of hepatocellular carcinoma (HCC) has progressively increased in recent years and is now the fifth and the second most common cancer in the World and in Egypt, respectively. Much work has focused in the development of assays for detecting hepatic carcinogensis before the observance of hepatic focal lesions. Particular attention has been directed towards HCC-specific biomarkers for use in the early diagnosis of HCC and in the confirmation of radiological studies. Although a number of biomarkers have been identified, none have been considered reliable indicators of early HCC lesions. This review presents a few of the most relevant HCC biomarkers and suggests improvements to the accuracy of diagnostic assays through their combined use. Furthermore, we present an algorithm for the biomarker-based diagnosis of HCC and highlight its important role in the early prediction of HCC. Core tip: Alpha-fetoprotein (AFP) has been widely used as a reference biomarker to validate the diagnosis of hepatocellular carcinoma (HCC). However, normal physiological-levels of AFP are observed in approximately one third of HCC cases. Furthermore, a number of HCC positive patients have AFP levels less than the threshold value of 400 ng/mL. These factors make an AFP-based diagnosis of HCC far from reliable. However, high diagnostic accuracy indices have been reported when AFP is combined with other biomarkers such as midkine, golgi protein 73, des-γ-carboxyprothrombin, glypican-3, and gamma-glutamyl transferase.Khattab M, Fouad M, Ahmed E. Role of biomarkers in the prediction and diagnosis of hepatocellular carcinoma. World J Hepatol 2015; 7(23): 24742481 Available from:

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Serum heat shock protein 27 levels in patients with hepatocellular carcinoma

Cited by 27 publications

References 22 publications

Serum Biomarkers Identification by Mass Spectrometry in High-Mortality Tumors

Serum Biomarkers Identification by Mass Spectrometry in High-Mortality Tumors

Mitogen-activated protein kinase-activated protein kinase 2 mediates resistance to hydrogen peroxide-induced oxidative stress in human hepatobiliary cancer cells

Role of biomarkers in the prediction and diagnosis of hepatocellular carcinoma

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