Protein profiling employing capillary electrophoresis with dendrimers as pseudostationary phase media

Stathakis, Costas; Arriaga, Edgar A.; Dovic̀hi, Norman J.

doi:10.1016/s0021-9673(98)00370-7

Cited by 37 publications

(26 citation statements)

References 21 publications

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“…The selectivity was different from that observed with micelles as PSP. Stathakis et al [49] used anionic and cationic poly(amido-amine) (PAMAM) dendrimers for separation of chicken sarcoplasmic proteins. Resolution improved as dendrimer concentration was increased.…”

Section: Dendrimersmentioning

confidence: 99%

Nanoparticle‐based pseudostationary phases in capillary electrochromatography

2006

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During the past decades, research has been performed to enhance selectivity in CE by introducing different types of additives into the electrolyte. Research concerning this has taken many directions, especially during the last 5 years. A promising technique, which benefits from no packing or frits, is to use nanoparticles as the pseudostationary phase (PSP) in CEC. PSPs have the advantage of introducing a novel interaction phase for every analysis, which greatly simplify column exchange and circumvent contamination inherited from complex mixtures, e.g., biological samples. The field of nanoparticle-based PSPs used in CEC is covered in this review. The term CEC will be used consequently throughout this review, although some authors used the term EKC to categorize their work. Important requirements for the nanoparticles used and possible reasons for band broadening will be discussed. Applications with silica nanoparticles, polymer nanoparticles, molecularly imprinted polymer nanoparticles, gold nanoparticles, dendrimers, and polymeric surfactants as PSP will also be discussed.

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Section: Dendrimersmentioning

confidence: 99%

Nanoparticle‐based pseudostationary phases in capillary electrochromatography

2006

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“…The application of dendrimers in electroseparation can be dated back to the pioneer works of Tanaka et al [9], in which they were employed as pseudostationary phase (PSP) for capillary electrochromatography. They were lately applied to capillary electrophoretic analysis of aromatic hydrocarbons [10], amino acids [1], parabens [11], proteins [12] and positional isomers of neutral phenols [13]. It was proposed that dendrimers' uniform surface charge density and structural homogeneity are mainly responsible for the decrease of plate height relative to the conventional cationic or anionic surfactants [1,10,14].…”

Section: Introductionmentioning

confidence: 99%

“…It was proposed that dendrimers' uniform surface charge density and structural homogeneity are mainly responsible for the decrease of plate height relative to the conventional cationic or anionic surfactants [1,10,14]. Dovichi et al [12] reported that in a neutral phosphate buffer (pH 7.2) the resolution of chicken sarcoplasmic proteins was improved in the presence of anionic half-generation polyamidoamine (PAMAM) dendrimers, but they found that analysis time enormously prolonged in the presence of cationic entire-generation PAMAM.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the influence of PAMAMs as additives on separation performance varies with buffer pH. On the other hand, buffers of wide pH range have been employed for high performance separation of proteins by CE/CEC, from extremely acidic [15], mild acidic [16][17][18][19][20] to neutral [12,[21][22][23][24], mild alkaline [25,26] and extremely alkaline [27]. Therefore, examining the properties of PAMAM dendrimers as additives in different pHs is helpful in extending their potential applications in protein analysis.…”

Section: Introductionmentioning

confidence: 99%

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Cationic poly(amidoamine) dendrimers as additives for capillary electroseparation and detection of proteins

et al. 2011

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We examine the influence of cationic poly(amidoamine) (PAMAM) dendrimers on capillary electroseparation-UV analysis of proteins. PAMAMs adsorbing to the capillary surface suppressed the wall-adsorption of proteins; meanwhile, PAMAMs added to the buffer exhibited selectivity toward proteins. Presence of 3 x 10⁻⁴ g/mL PAMAM generation one (G 1.0) in 30 mM phosphate, at pH 2.6, rendered significant enhancement in separation efficiency; the merged peaks of myoglobin and trypsin inhibitor were separated. Moreover, the protein-dendrimer interactions changed the inherent UV absorbance profiles of proteins. UV-Vis study showed that the absorbance of cytochrome C and transferrin increased at the detection wavelength of 214 nm; their detection sensitivity enhanced by 2.44 and 2.01-folds, respectively, with addition of 5 x 10⁻⁴ g/mL PAMAM G 1.0.

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“…34 It was also shown in the literature that polycationic dendrimes are strongly adsorbed on capillary walls. 35 From a large offer of commercially available dendrimers for experiments with modification of BGE the poly[propyleneimine] tetraammonium dendrimer generation 1.0 (DAB) was employed, and as model plant material for obtaining protein profile a commercially available soybean (SB) was employed.…”

Section: Dendrimers With Amine Core As Bge Modifiersmentioning

confidence: 86%

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

et al. 2011

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Abstract. The capillary electrophoresis with UV detection was employed for protein profiling in extracts from maize and soybeans. Modifications of back-ground electrolyte and coating the capillary wall with polybrene was employed in order to decrease the protein adsorption on the capillary walls. The obtained protein profiles were compared for transgenic and non-transgenic variants, showing in some cases significant changes that might be employed for identification of genetic modifications of plants or foods of plant origin. (doi: 10.5562/cca1777)

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Protein profiling employing capillary electrophoresis with dendrimers as pseudostationary phase media

Cited by 37 publications

References 21 publications

Nanoparticle‐based pseudostationary phases in capillary electrochromatography

Nanoparticle‐based pseudostationary phases in capillary electrochromatography

Cationic poly(amidoamine) dendrimers as additives for capillary electroseparation and detection of proteins

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

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