Protein profile and physicochemical characteristics of meat of lambs fed diets supplemented with rapeseed oil, fish oil, carnosic acid, and different chemical forms of selenium

Abstract

Abstract. The objective of this study was to evaluate the physicochemical characteristics of the longissimus muscle of lambs fed a control diet containing 3 % rapeseed oil (RO) (group I); an experimental diet with 2 % RO and 1 % fish oil (FO) (group II); or experimental diets with coupled addition of 2 % RO, 1 % FO, and 0.1 % carnosic acid (CA) (group III) without/with 0.35 ppm Se as selenized yeast (SeY) (group IV) or selenate (SeVI as sodium selenate, Na 2 SeO 4 ) (group V). The results showed that dietary FO or SeVI affected the ultimate pH and meat color in comparison to lambs fed the control diet. The differences noted between diets in the case of muscle tissue were as follows: myosin HC (heavy chain) and LC1 (light chain), 150 kDa, α-actinin, 60 kDa, and TnT (troponin T), 30 kDa protein. The addition of FO to lamb diets resulted in a lower content of 30 kDa proteins, which are indicators of proteolysis. Considering the protein profile of drip loss, results showed effects of dietary administration of FO, CA, and Se (as SeY and SeVI) on the abundance of the following sarcoplasmic proteins: AMPDA (AMP deaminase), PGM (phosphoglucomutase), PK/PGI (pyruvate kinase/phosphoglucose isomerase), CK/PGAK (creatine kinase/phosphoglycerate kinase), ALD (aldolase), LDH (lactate dehydrogenase), PGAM (phosphoglycerate mutase), and TPI (triosephosphate isomerase). The lower content of TPI (in group II with comparison to groups III-V) and PGAM (in group II with comparison to group I and III) could be due to a higher ultimate pH in the group with FO addition, whereas lower contents of CK/PGAK, ALD, and LDH were attributed to Se addition. In comparison to the control diet, all experimental diets without SeVI decreased the content of the sum of all assayed amino acids ( AAs), indispensable amino acids ( IAAs), and dispensable amino acids ( DAAs) in the muscle. The highest ratios of IAAs to DAAs and IAAs to AAs were found in the muscle of lambs fed the diet containing RO, FO, and CA. The FO-supplemented diet with CA and SeVI increased the abundance of methionine in the muscle in comparison to the control diet and the FO-supplemented diet without/with CA.

Proximate Composition, Amino Acid Profile, and Mineral Content of Four Sheep Meats Reared Extensively in Morocco: A Comparative Study

“…This result could be explained by the livestock system since the BG and ODj sheep were reared in the same cradle, same livestock system, and practically same feeding. In comparison with the reported results by Polidori et al [ 21 ] in the meat of Fabrianese sheep breed and Przybylski et al [ 50 ] in the meat of Corriedale lambs, the studied meats had a high protein quality and are rich in essential amino acids. The nature of the breeding system adopted by Moroccan breeders was based mainly on pasture rich in greenery (rich in nitrogenous materials) such as Artemisia herba-alba , Stipa tenacissima, and Atriplex halimus , which can explain this recorded wealth [ 51 – 53 ].…”

Diets enriched in fish and rapeseed oils, carnosic acid, and different chemical forms of selenium affect fatty acid profile in the periintestinal fat and indices of nutritional properties of selected tissues of lambs

“…Our studies show that short-term dietary supplementation of CA and Se as VI Se or Y Se (rich in Se-Met) can be used to modify the concentration of FA, TCh and tocopherols in the PIF, SCF and muscles of lambs without adversely influencing performance or causing physiological disorders in the liver, blood and brain (Eun et al 2013, Rozbicka-Wieczorek et al 2016b, 2016c, Krajewska-Bienias et al 2017, Przybylski et al 2017, Białek et al 2018. Se-compounds are metabolised to intermediates and then utilised for the biosynthesis of Se-Cys-or Se-Met-containing proteins/enzymes in the tissues of mammals (Collins 2017).…”

“…The oxidation of polyunsaturated fatty acids (PUFA), cholesterol and proteins in tissues is one of the most prevalent modifications caused by reactive oxygen and nitrogen species (ROS and RNS) in animal organisms (Saheem et al 2017). Numerous studies have indicated that ruminants' tissues are susceptible to oxidation of lipid unsaturated fatty acids (UFA), sulphur-amino acids in proteins or cholesterol in cell membranes (Krajewska-Bienias et al 2017, Morán et al 2017, Przybylski et al 2017. Dietary antioxidants (like seleno-compounds, polyphenols, carotenoids, lycopene or tocopherols) may prevent or delay some types of oxidative damage in tissues of animals and humans (Choe and Min 2009, El-Ramady et al 2015, Gargiulo et al 2017.…”

The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation

“…Also, Fu et al (2014) showed a significant influence of stress on the level of glycolytic enzymes such as: Glucose-6-phosphate isomerase, 6-Phosphofructokinase, Glyceraldehyde-3-phosphate dehydrogenase, Phosphoglycerate mutase, Pyruvate kinase, Lactate dehydrogenase, Creatine kinase. Some studies have shown that nutritional factors can significantly affect the sarcoplasmic protein profile and glycolytic enzyme abundance ( Shibata et al, 2009 ; Przybylski et al, 2017 ; Watanabe et al 2020 ). The study of Watanabe et al (2020) showed that reduction in dietary lysine (from 100% to 90% recommended requirement) upregulated 22 metabolites (among them, inter alia, related to glycolytic transformations) and 24 were downregulated.…”