Protein phosphorylation in Nicotiana tabacum cells in response to perception of lipopolysaccharides from Burkholderia cepacia

Gerber, Isak B.; Dubery, Ian A.

doi:10.1016/j.phytochem.2004.09.005

Cited by 31 publications

(25 citation statements)

References 30 publications

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“…Despite the vast differences in their lifestyles, these plant pathogens share a common dilemma: They need to physically interact with the host plant, thereby generating wounds and releasing components from the plant surface, which allow the plant to recognize the invasion. Furthermore, exposed microbial surface structures and essential components, such as the major protein of bacterial flagellae, flagellin (Gomez-Gomez and Boller, 2002), or the outermembrane lipooligosaccharides of gram-negative bacteria (Gerber and Dubery, 2004;Gross et al, 2005), can serve as vehicles to launch the plant defense response. These molecules possess crucial basic functions for the microbe without being specifically intended for the interaction and have been termed microbe-or pathogen-associated molecular patterns (MAMPs/PAMPs; Nü rnberger and Lipka, 2005).…”

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confidence: 99%

A Single Binding Site Mediates Resistance- and Disease-Associated Activities of the Effector Protein NIP1 from the Barley Pathogen Rhynchosporium secalis

2007

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The effector protein NIP1 from the barley (Hordeum vulgare) pathogen Rhynchosporium secalis specifically induces the synthesis of defense-related proteins in cultivars of barley expressing the complementary resistance gene, Rrs1. In addition, it stimulates the activity of the barley plasma membrane H 1 -ATPase in a genotype-unspecific manner and it induces necrotic lesions in leaf tissues of barley and other cereal plant species. NIP1 variants type I and II, which display quantitative differences in their activities as elicitor and H 1 -ATPase stimulator, and the inactive mutant variants type III* and type IV*, were produced in Escherichia coli. Binding studies using 125 I-NIP1 type I revealed a single class of binding sites with identical binding characteristics in microsomes from near-isogenic resistant (Rrs1) and susceptible (rrs1) barley. Binding was specific, reversible, and saturable, and saturation ligand-binding experiments yielded a K d of 5.6 nM. A binding site was also found in rye (Secale cereale) and the nonhost species wheat (Triticum aestivum), oat (Avena sativa), and maize (Zea mays), but not in Arabidopsis (Arabidopsis thaliana). For NIP1 types I and II, equilibrium competition-binding experiments revealed a correlation between the difference in their affinities to the binding site and the differences in their elicitor activity and H 1 -ATPase stimulation, indicating a single target molecule to mediate both activities. In contrast, the inactive proteins type III* and type IV* are both characterized by high affinities similar to type I, suggesting that binding of NIP1 to this target is not sufficient for its activities.

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confidence: 99%

A Single Binding Site Mediates Resistance- and Disease-Associated Activities of the Effector Protein NIP1 from the Barley Pathogen Rhynchosporium secalis

2007

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“…[15][16][17] However, some of the responses seemed to be specific to LPS of the pathogen Pectobaterium (e.g. PCD), or non-pathogenic bacteria (e.g., medium alkalization), when other shared responses (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…14 Nevertheless, LPS from different bacterial species can trigger or potentiate PTI and several studies show their implication into the mechanism of plant defense activation. [15][16][17] Lipopolysaccharides have been reported to induce an oxidative burst, NO production, calcium influx, alkalization of the extracellular medium, pathogenesis-related (PR) gene expression and cell wall alterations that include the deposition of callose and phenolics.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the dual effect of lipopolysaccharides from plant pathogenicPectobacterium

Kettani-Halabi

Tran

Dauphin

et al. 2015

Plant Signaling & Behavior

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“…These analyses suggest that the resistance mechanism in «Hydra» is more efficient than in «Bysra», since it affects all the tested components. The difference of the resistance mechanism could be due to the protein phosphorylation which plays an important regulatory role in the delivery and response to different biotic stress signals, and also induces a variety of stress signal-induced gene expressions (Gerber and Dubery, 2004). The cultivar «Hydra» could also affect the number of pathogen genotypes with matching virulence gene(s) that they may possibly not proliferate during the vegetation season to substantial level in the population and in turn would not enhance epidemic level of the disease.…”

Section: Discussionmentioning

confidence: 99%

Response of Tunisian Durum and Common Wheat Cultivars to Powdery Mildew under Natural Infection

Hammami¹,

Mansouri²,

Rassaa³

et al. 2014

Turkish Journal of Field Crops

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Cereal production is an important component of agriculture. Powdery mildew (Erysiphe graminis f. sp tritici Em. Marchal), caused by Blumeria graminis (DC.) E.O Speerf. sp. tritici (Bgt), which is one of the most worldwide devastating diseases of durum and common wheat specially in Tunisia. Several works have discussed plant resistance to mildew and its potential risks but, it was neglected or no study was reported for that in Tunisia. There is an increasing need for studies that address the potential side effects of mildew to different wheat cultivars and their outcome and behave via disease criteria. This study focuses to compare the behave of ten Tunisian wheat cultivars to powdery mildew under field conditions during two successive cropping seasons 2011-2013. Using the 5 disease criteria (infected leaf number, pustules per stem and per leaf, number of spores produced per pustule and AUDPC "Area Under the Disease Progress Curve", based on pustules per leaf) to investigate the plant disease resistance, specially the AUDPC parameter, durum wheat showed susceptibility to natural infection of powdery mildew specially the cultivar Souabaa Eljia with mean AUDPC value of 583.8, while common wheat, the cultivar Hydra, showed resistance with mean AUDPC value of 52.1,while, the improved durum cultivars Nasr and Karim showed an intermediate reaction. Principal Components Analysis (PCA) showed that the variables were grouped in two principal components that explained 76.65% of the total variance. The projection of the point-cloud representing the populations on the plan formed by the principal components «Fact.1» (49.65%), in abscissa, and «Fact.2» (27%) in ordinate, permitted to distribute the accessions in several groups. Two common wheat cultivars (Hydra and Bysra) showed on one hand a high level of resistance to Bgt and on other hand this fungus behaves differently on these cultivars via the conidia production. The cluster analysis suggested that these two cultivars are genetically similar, but the PCA distribute each one in a distinct single group. Hydra affect all the component of resistance tested and seems more efficient than Bysra.

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Protein phosphorylation in Nicotiana tabacum cells in response to perception of lipopolysaccharides from Burkholderia cepacia

Cited by 31 publications

References 30 publications

A Single Binding Site Mediates Resistance- and Disease-Associated Activities of the Effector Protein NIP1 from the Barley Pathogen Rhynchosporium secalis

A Single Binding Site Mediates Resistance- and Disease-Associated Activities of the Effector Protein NIP1 from the Barley Pathogen Rhynchosporium secalis

Deciphering the dual effect of lipopolysaccharides from plant pathogenicPectobacterium

Response of Tunisian Durum and Common Wheat Cultivars to Powdery Mildew under Natural Infection

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