Deciphering the dual effect of lipopolysaccharides from plant pathogenic<i>Pectobacterium</i>

Kettani-Halabi, Mohamed; Tran, Daniel; Dauphin, Aurélien; El-Maarouf-Bouteau, Hayat; Errakhi, Rafik; Arbelet-Bonnin, Delphine; Biligui, Bernadette; Mustapha, Ennaji Moulay; François, Bouteau

doi:10.1080/15592324.2014.1000160

Cited by 12 publications

(8 citation statements)

References 80 publications

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“…Previous research reported that different defense response patterns could be induced by the LPS of P. atrosepticum and Pseudomonas corrugata in three Solanaceae species, including tobacco, tomato, and potato [84]. Additionally, different signaling pathways could also be activated by LPS in Arabidopsis thaliana cells [85]. A previous study showed that LPS are crucial for the optimal growth, survival and virulence of P. atrosepticum [86], but the roles of LPS in the SX309 strain remain to be determined.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of Pectobacterium carotovorum subsp. brasiliense SX309 provides novel insights into its genetic and phenotypic features

Yuan

Shi

et al. 2019

BMC Genomics

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Background Pectobacterium carotovorum subsp. brasiliense is a broad host range bacterial pathogen, which causes blackleg of potatoes and bacterial soft rot of vegetables worldwide. Production of plant cell wall degrading enzymes is usually critical for Pectobacterium infection. However, other virulence factors and the mechanisms of genetic adaptation still need to be studied in detail. Results In this study, the complete genome of P. carotovorum subsp. brasiliense strain SX309 isolated from cucumber was compared with eight other pathogenic bacteria belonging to the Pectobacterium genus, which were isolated from various host plants. Genome comparison revealed that most virulence genes are highly conserved in the Pectobacterium strains, especially for the key virulence determinants involved in the biosynthesis of extracellular enzymes and others including the type II and III secretion systems, quorum sensing system, flagellar and chemotactic genes. Nevertheless, some variable regions of the T6SS and the CRISP-Cas immune system are unique for P. carotovorum subsp. brasiliense . Conclusions The extensive comparative genomics analysis revealed highly conserved virulence genes in the Pectobacterium strains. However, several variable regions of type VI secretion system and two subtype Cas mechanism-Cas immune systems possibly contribute to the process of Pectobacterium infection and adaptive immunity. Electronic supplementary material The online version of this article (10.1186/s12864-019-5831-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of Pectobacterium carotovorum subsp. brasiliense SX309 provides novel insights into its genetic and phenotypic features

Yuan

Shi

et al. 2019

BMC Genomics

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“…In Arabidopsis cells, different kinetics of the ROS burst were observed 97 using LPS derived from pathogenic and nonpathogenic bacteria, and ROS were 98 detected in the cytosol (Mohamed et al, 2015). These variable immune responses 99 present a challenge regarding identification of the common plant components 100 involved in LPS recognition and the related signal transduction events.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharides Trigger Two Successive Bursts of Reactive Oxygen Species at Distinct Cellular Locations

et al. 2018

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“…A homologue of wcaG gene is also found in Pectobacterium carotovorum that encodes GDP fucose synthetase-like protein and has been annotated as NAD-dependent epimerase/dehydratase. Recently, the exopolysaccharide, colanic acid (CA) and lipopolysaccharide (LPS) have been reported to be associated with virulence in Pectobacterium as the purified molecules induced pathogenicity-related physiological responses such as cell death and generation of reactive oxygen species in host cells [20]. Here, we demonstrate that mutation in wcaG gene in Pectobacterium carotovorum strain Ecc71 reduces PCWDEs production and virulence remarkably and affects cell surface properties including biofilm formation and cell surface hydrophobicity.…”

Section: Introductionmentioning

confidence: 74%

The Gene Encoding NAD-Dependent Epimerase/Dehydratase, wcaG, Affects Cell Surface Properties, Virulence, and Extracellular Enzyme Production in the Soft Rot Phytopathogen, Pectobacterium carotovorum

et al. 2019

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Pectobacterium carotovorum is a gram-negative bacterium that, together with other soft rot Enterobacteriaceae causes soft rot disease in vegetables, fruits, and ornamental plants through the action of exoproteins including plant cell wall-degrading enzymes (PCWDEs). Although pathogenicity in these bacteria is complex, virulence levels are proportional to the levels of plant cell wall-degrading exoenzymes (PCWDEs) secreted. Two low enzyme-producing transposon Tn5 mutants were isolated, and compared to their parent KD100, the mutants were less virulent on celery petioles and carrot disks. The inactivated gene responsible for the reduced virulence phenotype in both mutants was identified as wcaG. The gene, wcaG (previously denoted fcl) encodes NAD-dependent epimerase/dehydratase, a homologue of GDP-fucose synthetase of Escherichia coli. In Escherichia coli, GDP-fucose synthetase is involved in the biosynthesis of the exopolysaccharide, colanic acid (CA). The wcaG mutants of P. carotovorum formed an enhanced level of biofilm in comparison to their parent. In the hydrophobicity test the mutants showed more hydrophobicity than the parent in hexane and hexadecane as solvents. Complementation of the mutants with extrachromosomal copies of the wild type gene restored these functions to parental levels. These data indicate that NAD-dependent epimerase/dehydratase plays a vital rule in cell surface properties, exoenzyme production, and virulence in P. carotovorum.

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Deciphering the dual effect of lipopolysaccharides from plant pathogenicPectobacterium

Cited by 12 publications

References 80 publications

Comparative genomic analysis of Pectobacterium carotovorum subsp. brasiliense SX309 provides novel insights into its genetic and phenotypic features

Comparative genomic analysis of Pectobacterium carotovorum subsp. brasiliense SX309 provides novel insights into its genetic and phenotypic features

Lipopolysaccharides Trigger Two Successive Bursts of Reactive Oxygen Species at Distinct Cellular Locations

The Gene Encoding NAD-Dependent Epimerase/Dehydratase, wcaG, Affects Cell Surface Properties, Virulence, and Extracellular Enzyme Production in the Soft Rot Phytopathogen, Pectobacterium carotovorum

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