Protein microarrays and quantum dot probes for early cancer detection

Zajac, Aleksandra; Song, Dongzhe; Qian, Wei; Zhukov, Tatyana

doi:10.1016/j.colsurfb.2007.02.019

Cited by 115 publications

(63 citation statements)

References 23 publications

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“…Functionalized semiconductor QDs have been used as fluorescence labels in numerous biorecognition events, [60][61][62][63][64][65] such as immunoassays for protein detection (Figure 3 A) or the analysis of nucleic acids (Figure 3 B). For example, CdSe/ ZnS QDs were functionalized with avidin groups and used as fluorescent labels for biotinylated antibodies.…”

Section: Water Solubilization and Functionalization Of Quantum Dots Wmentioning

confidence: 99%

Semiconductor Quantum Dots for Bioanalysis

2008

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Semiconductor nanoparticles, or quantum dots (QDs), have unique photophysical properties, such as size-controlled fluorescence, have high fluorescence quantum yields, and stability against photobleaching. These properties enable the use of QDs as optical labels for the multiplexed analysis of immunocomplexes or DNA hybridization processes. Semiconductor QDs are also used to probe biocatalytic transformations. The time-dependent replication or telomerization of nucleic acids, the oxidation of phenol derivatives by tyrosinase, or the hydrolytic cleavage of peptides by proteases are probed by using fluorescence resonance energy transfer or photoinduced electron transfer. The photoexcitation of QD-biomolecule hybrids associated with electrodes enables the photoelectrochemical transduction of biorecognition events or biocatalytic transformations. Examples are the generation of photocurrents by duplex DNA assemblies bridging CdS NPs to electrodes, and by the formation of photocurrents as a result of biocatalyzed transformations. Semiconductor nanoparticles are also used as labels for the electrochemical detection of DNA or proteins: Semiconductor NPs functionalized with nucleic acids or proteins bind to biorecognition complexes, and the subsequent dissolution of the NPs allows the voltammetric detection of the related ions, and the tracing of the recognition events.

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Section: Water Solubilization and Functionalization Of Quantum Dots Wmentioning

confidence: 99%

Semiconductor Quantum Dots for Bioanalysis

2008

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“…These QDs also are used for real-time in vitro [34] and in vivo [35] optical imaging of cancer cells. Cancer biomarkers such as prostate-specific antigen [36], HER2 [37], CD44 [38], and folic acid [39] specifically present in the tumor microenvironment or on the cancer cells surface, can be recognized by the appropriate monoclonal antibody [40], peptide [41], or immunoglobulin [18,42] that are conjugated onto the QDs surface ( Figure 4).…”

Section: Qds As Contrast Agents For Optical Imagingmentioning

confidence: 99%

A Review on Aptamer-Conjugated Quantum Dot Nanosystems for Cancer Imaging and Theranostic

Johari-Ahar¹

2017

JNMR

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Over the last 10 years, fluorescent semiconductor QD (quantum dot)-aptamer conjugates have emerged as an efficient platform for cancer imaging and therapy in animal models and in vitro. In addition, these conjugates show potential in a wide range of applications in environmental monitoring, disease diagnosis, and bio-sensing. The present review represents the recent developments in QDaptamer bio-conjugates for applications in cancer studies. It starts with a brief introduction to Semiconductor Quantum dots (QDs), bio-conjugation of QDs and aptamer molecules, and advantages-disadvantages of using these novel tools for biochemical applications.

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“…In this study, three advances in optical protein biosensor are presented: 1) without enzymatic signal amplification, the surface immobilized optical protein sensor can reach a LOD of detecting IL-8 at 1.1 pM in buffer, 2) with the utilization of confocal optics, the LOD of this sensor can be further reduced by two orders of magnitude (Schweitzer et al, 2002, Zajac et al, 2007, detecting IL-8 at 4.0 fM in buffer and 3) the optical protein sensor is validated through detecting IL-8 protein in clinical saliva samples by comparing with traditional ELISA measurements. Our results show that the patients with oral cancer can be clearly distinguished from the control subjects.…”

Section: Introductionmentioning

confidence: 99%

Optical protein sensor for detecting cancer markers in saliva

Tan

Sabet

et al. 2008

Biosensors and Bioelectronics

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A surface immobilized optical protein sensor has been utilized to detect Interleukin-8 (IL-8) protein, an oral cancer marker, and can reach limit of detection (LOD) at 1.1 pM in buffer without using enzymatic amplification. Only after applying enzymatic amplification to increase the signal level by a few orders of magnitude, ELISA can reach the LOD of 1pm level. We then develop the confocal optics based sensor for further reducing the optical noise and can extend the LOD of the surface immobilized optical protein sensor two orders in magnitude. These improvements have allowed us to detect IL-8 protein at 4.0 fM in buffer. In addition, these sensitive LODs were achieved without the use of enzymatic signal amplification, such that the simplified protocol can further facilitate the development of point-of-care devices.The ultra sensitive optical protein sensor presented in this paper has a wide number of applications in disease diagnoses. Measurements for detecting biomarkers in clinical sample are much more challenging than the measurements in buffer, due to high background noise contributed by large collections of non-target molecules. We used clinical salvia samples to validate the functionality of the optical protein sensor. Clinical detection of disease-specific biomarkers in saliva offers a noninvasive, alternative approach to using blood or urine. Currently, the main challenge of using saliva as a diagnostic fluid is its inherently low concentration of biomarkers. We compare the measurements of 40 saliva samples; half from oral cancer patients and half from a control group. The data measured by the optical protein sensor is compared with the traditional Enzyme-Linked Immunosorbant Assay (ELISA) values to validate the accuracy of our system. These positive results enable us to proceed to using confocal optical protein sensor to detect other biomarkers, which have much lower concentrations.

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Protein microarrays and quantum dot probes for early cancer detection

Cited by 115 publications

References 23 publications

Semiconductor Quantum Dots for Bioanalysis

Semiconductor Quantum Dots for Bioanalysis

A Review on Aptamer-Conjugated Quantum Dot Nanosystems for Cancer Imaging and Theranostic

Optical protein sensor for detecting cancer markers in saliva

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