Protein metabolism in Spodoptera litura (F.) is influenced by the botanical insecticide azadirachtin

Huang, Zhiwei; Shi, Ping; Dai, J.; Du, Jiawei

doi:10.1016/j.pestbp.2004.07.001

Cited by 78 publications

(45 citation statements)

References 32 publications

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“…The females of Chrotogonus homalodemus (Blanch) (Orthoptera: Acrididae) showed greater reluctant feeding behavior than the males to neem extracts (Grahn, 1993). The females of Spodoptera litura (F.) (Lepidoptera: Noctuidae) feeding on the artificial diets showed a stronger response to azadirachtin, with large differences between the controls and treated diet compared to the males (Huang et al, 2004). The females of the parasitoids Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) were affected by the neem seed oil but the males were unaffected in the contact toxicity tests (Raguraman and Singh, 1999).…”

Section: Multiple-choice Assaysmentioning

confidence: 99%

Repellent, antifeedant and insecticidal effects of neem oil on Microtheca punctigera

Mikami

Ventura

2008

Braz. arch. biol. technol.

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Section: Multiple-choice Assaysmentioning

confidence: 99%

Repellent, antifeedant and insecticidal effects of neem oil on Microtheca punctigera

Mikami

Ventura

2008

Braz. arch. biol. technol.

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“…The reduced level of ALP suggests that energy metabolism may have been directly affected by cantharidin, by reducing the level of phosphorus liberation that in turn decreases metabolism as well as the transport of metabolites. Similar effects of azadirachtin and nucleopolyhedrovirus were observed on ALP and other digestive enzymes (Huang et al, 2004;Senthil-Nathan et al, 2005).…”

Section: Discussionmentioning

confidence: 64%

Molecular and biochemical characterization of the effects of insecticidal toxin from meloidae beetles on Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae)

Khan¹,

Rashid²,

Wang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The molecular and biochemical effects of an insecticidal toxin extracted from Meloidae beetles were investigated on Helicoverpa armigera. The toxin was identified as cantharidin, a well-known natural compound produced by beetles of family Meloidae and Oedemeridae. Furthermore, the effect of the toxin on the metabolic enzymes alkaline phosphatase (ALP) and glutathione S-transferase (GST), responsible for the metabolism of insecticides, was also investigated. Results of a diet incorporation bioassay performed under laboratory conditions showed that the LC 50 value of cantharidin was 0.068 mg/g. The body weight of the insect was also significantly reduced by cantharidin treatment. The LC 10 concentration of cantharidin, 0.01 mg/g, was also tested to determine its effect on ALP and GST. Our results showed that cantharidin significantly inhibited ALP activity after 48 h, whereas GST activity was significantly inhibited after 24 h. The decline of ALP and GST transcript levels was also validated by semiquantitative RT-PCR analysis. It may be concluded from the results that ALPs and GSTs may be targets of the cantharidin intoxication mechanism. Moreover, the inability of ALP and GST to metabolize cantharidin shows that the mechanism of detoxification for cantharidin is different from that for conventional insecticides. On the basis of our investigations, the chemical structure of insecticides may be modified using a model structure of cantharidin, to avoid metabolism by metabolic enzymes.

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“…Overall, the depletion of energy reserves could be due to a reduction in plant consumption, or to high mobilization of these primary metabolites to compensate the metabolic stress produced by the toxic effects of the plant extracts. A depression of energy reserves has also been reported after ingestion of Jatropherol-I by B. mori (Jing et al, 2005), neem oil by Choristoneura rosaceana (Smirle et al, 1996), P. harmala leaves by T. castaneum , and azadirachtin by some insects such as S. litura (Huang et al, 2004). Moreover, reductions in energy reserves were reported in P. interpunctella after ingestion of harmine (Bouayad et al, 2012), harmaline (Rharrabe et al, 2007), azadirachtin and 20-hydroxyecdysone (Rharrabe et al, 2009).…”

Section: Discussionmentioning

confidence: 88%

Insecticidal effects of Moroccan plant extracts on development, energy reserves and enzymatic activities of Plodia interpunctella

Bouayad

Rharrabe

Ghailani

et al. 2012

Span. j. agric. res.

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The aim of this work was to study the effects of methanol extracts of ten plant species used in traditional medicine in Morocco (Peganum harmala, Ajuga iva, Rosmarinus officinalis, Lavandula stoechas, Lavandula dentata, Cistus ladanifer, Cistus salviaefolius, Cistus monspeliensis, Centaurium erythraea and Launaea arborescens) on Plodia interpunctella Hübner (Lepidoptera: Pyralidae) larvae. Firstly, we studied the effects of the ingestion of these extracts at 500 ppm on post-embryonic development parameters. Most plant extracts provoked a notable decrease of larval weight 8 days after treatment (up to 33% weight loss with C. erythraea) and caused significant alterations on pupation (ranging from 5% to 85%) and adult emergence (below 2.5% with R. officinalis, C. erythraea and A. iva). The plant extracts that showed strongest effects on post-embryonic development were selected to test their effects on the following physiological parameters: larval reserve substances (at 500 ppm); and midgut activities of hydrolytic and detoxification enzymes (at 500, 750 and 1000 ppm). All treatments provoked a significant reduction of protein and carbon hydrate larval contents, the inhibition of proteases and α-amylase activities in a dose depended manner, and the induction of glutathione S-transferase and esterase (using MtB as substrate) activities, whereas the activity of cytochrome P450 monooxygenases and esterases (using 1-NA as substrate) increase or decrease depending on the extract concentration and the plant analyzed.

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Protein metabolism in Spodoptera litura (F.) is influenced by the botanical insecticide azadirachtin

Cited by 78 publications

References 32 publications

Repellent, antifeedant and insecticidal effects of neem oil on Microtheca punctigera

Repellent, antifeedant and insecticidal effects of neem oil on Microtheca punctigera

Molecular and biochemical characterization of the effects of insecticidal toxin from meloidae beetles on Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae)

Insecticidal effects of Moroccan plant extracts on development, energy reserves and enzymatic activities of Plodia interpunctella

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