Protein metabolism during natural ageing in desiccating recalcitrant seeds of Shorea robusta

Parkhey, Suruchi; Naithani, S. C.; Keshavkant, S.

doi:10.1007/s11738-014-1540-x

Cited by 31 publications

(31 citation statements)

References 39 publications

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“…In agreement, similar results were also published for tissues of Quercus robur and Pisum sativum when were kept under defined conditions by Ntuli et al (2011;2013). Desiccation implies over production of ROS in seeds kept under storage (Pukacka and Ratajczak 2006;Parkhey et al 2014a). Like ours (Fig.…”

Section: Discussionsupporting

confidence: 69%

“…Our data depicted an inverse relationship (r=−94, P<0.05) between DT capacity and MDA accumulation. In agreement to Ntuli et al (2013) and Parkhey et al (2014a), C. arietinum L. exhibited considerable (P<0.05) fall in protein content under both SD and WS, but was maintained high enough throughout the RD (Fig. 3a).…”

Section: Discussionsupporting

confidence: 68%

“…It has already been suggested that during desiccation, electron transport chain of mitochondrial and microsomal membranes becomes disrupted, which results in reactive oxygen species (ROS)-induced oxidative situation that culminates with loss of seed viability (Parkhey et al 2012;2014a). Moreover, Song and co-workers (2009) recently reported a fall in the activity of cytochrome c oxidase in desiccating radicles and embryos of Antaris toxicaria and Zea mays respectively, allowing excessive accumulation of ROS in these tissues.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…It was shown to alter both structure and functions of proteins through oxidative modifications (Parkhey et al 2014a). Reactive oxygen species oxidizes to proteins by directly attacking on it following various strategies like carbonylation, nitrosylation, glutathionylation and disulphide bond formation with specific amino acids, and indirectly by making adducts of it with lipid peroxidized products such as MDA (Parkhey et al 2014a). Accumulation of large amount of protein hydroperoxide (PrOOH), a well recognized derivative of oxidized protein, has also been indicated during accelerated ageing (Keshavkant et al 2013) and desiccation (Parkhey et al 2014a) of seeds.…”

Section: Introductionmentioning

confidence: 99%

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Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

Chandra

Tandon

Keshavkant

2015

Physiol Mol Biol Plants

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Orthodox seed serves as easily accessible model to study desiccation-sensitivity in plant tissues because once they undergo germination, they become sensitive to desiccation imposed injuries. In the proposed study, effects of rate of drying on the viability, electrolyte leakage, superoxide accumulation, lipid-protein oxidation and antioxidant enzymes were explored in excised radicles of Cicer arietinum L. under dehydration and wet storage. For both the drying conditions, desiccation could be explained by exponential and inverse functions. Under rapid drying tissue viability as scored by germination efficiency and tetrazolium staining remained 100 % all through the analysis (24 h) but declined remarkably after 0.30 g g(-1) fresh mass water content (4 days) under slow drying. Moreover, precipitous fall in tissue viability was observed after 2 weeks of wet storage. Rapid drying was also accompanied with limited amounts of electrolyte leakage, superoxide radical, malondialdehyde and protein hydroperoxide, together with enhanced level of protein. Additionally, activities of both superoxide dismutase and ascorbate peroxidase were increased in rapidly dried radicles, but guaiacol peroxidase was declined. In contrary, above referred biomarkers were observed to perform either inversely or poorly during slow drying and wet storage suggesting that above documented alterations might be the resultant of ageing and not desiccation. Gathered data demonstrated that increased drying lowers the critical water content for tissue survival and also reduces the risk of damage resulting from aqueous-based deleterious reactions. Additionally, it also showed that growing radicles are a popular model to explore desiccation-sensitivity in plant tissues and/or seeds.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

Chandra

Tandon

Keshavkant

2015

Physiol Mol Biol Plants

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Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two‐dimensional gel electrophoresis

Parkhey

Chandrakar

Naithani

et al. 2015

J of Separation Science

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Protein extraction for two-dimensional electrophoresis from tissues of recalcitrant species is quite problematic and challenging due to the low protein content and high abundance of contaminants. Proteomics in Shorea robusta is scarcely conducted due to the lack of a suitable protein preparation procedure. To establish an effective protein extraction protocol suitable for two-dimensional electrophoresis in Shorea robusta, four procedures (borate buffer/trichloroacetic acid extraction, organic solvent/trichloroacetic acid precipitation, sucrose/Tris/phenol, and organic solvent/phenol/sodium dodecyl sulfate) were evaluated. Following these, proteins were isolated from mature leaves and were analyzed for proteomics, and also for potential contaminants, widely reported to hinder proteomics. The borate buffer/trichloroacetic acid extraction had the lowest protein yield and did not result in any banding even in one-dimensional electrophoresis. In contrast, organic solvent/phenol/sodium dodecyl sulfate extraction allowed the highest protein yield. Moreover, during proteomics, organic solvent/phenol/sodium dodecyl sulfate extracted protein resolved the maximum number (144) of spots. Further, when proteins were evaluated for contaminants, significant (77-95%) reductions in the nucleic acids, phenol, and sugars were discernible with refinement in extraction procedure. Accumulated data suggested that the organic solvent/phenol/sodium dodecyl sulfate extraction was the most effective protocol for protein isolation for proteomics of Shorea robusta and can be used for plants that have a similar set of contaminants.

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Inimical Effects of Arsenic on the Plant Physiology and Possible Biotechnological Solutions to Mitigate Arsenic-Induced Toxicity

Sharma

Monika

Goyal

et al. 2020

Contaminants in Agriculture

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Protein metabolism during natural ageing in desiccating recalcitrant seeds of Shorea robusta

Cited by 31 publications

References 39 publications

Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two‐dimensional gel electrophoresis

Inimical Effects of Arsenic on the Plant Physiology and Possible Biotechnological Solutions to Mitigate Arsenic-Induced Toxicity

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