Protein Mediated Magnetic Coupling between Lactate and Water Protons

Swanson, Scott D.

doi:10.1006/jmre.1998.1535

Cited by 34 publications

(46 citation statements)

References 27 publications

(21 reference statements)

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“…This could lead to a nonspecific off-resonance MT effect similar to that of water and the lactate H3 protons as observed previously (27,35). It is unknown whether such a mechanism is actually operative for 13 C. A previous study demonstrated that no tritium-tritium MT exists in a tritium-doped aqueous lipid bilayer system (37).…”

Section: Discussionmentioning

confidence: 80%

In vivo ¹³C saturation transfer effect of the lactate dehydrogenase reaction

Yang

Shen

2007

Magnetic Resonance in Med

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Lactate dehydrogenase (LDH, EC 1.1.1.27) catalyzes an exchange reaction between pyruvate and lactate. It is demonstrated here that this reaction is sufficiently fast to cause a significant magnetization (saturation) transfer effect when the 13 C resonance of pyruvate is saturated by a continuous-wave (CW) RF pulse. Infusion of [2-13 C]glucose was used to allow labeling of pyruvate C2 at 207.9 ppm to determine the pseudo first-order rate constant of the unidirectional lactate 3 pyruvate flux in vivo. During systemic administration of GABA A receptor antagonist bicuculline, this pseudo first-order rate constant was determined to be 0.08 ؎ 0.01 s ؊1 (mean ؎ SD, N ‫؍‬ 4) in halothane-anesthetized adult rat brains. In 9L and C6 rat glioma models, the 13 C saturation transfer effect of the LDH reaction was also detected in vivo. Our results demonstrate that the 13 C magnetization transfer effect of the LDH reaction may be useful as a novel marker for utilizing noninvasive in vivo MRS to study many physiological and pathological conditions, such as cancer. Magn Reson Med 57:258 -264, 2007.

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Section: Discussionmentioning

confidence: 80%

In vivo ¹³C saturation transfer effect of the lactate dehydrogenase reaction

Yang

Shen

2007

Magnetic Resonance in Med

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“…Whether this is due to a true immobilized lactate pool or via cross-relaxation including water molecules cannot concluded from these data. However, the presence of macromolecules from human plasma 68 or cross-linked bovine serum albumin (BSA) 70 in lactate phantoms is needed to produce correspondingly similar experimental results, suggesting water-mediated cross relaxation. The latter hypothesis is confirmed by MT from water to lactate as well as vice versa.…”

Section: Lactatementioning

confidence: 99%

“…The latter hypothesis is confirmed by MT from water to lactate as well as vice versa. 70 By this means lactate can be imaged indirectly from the difference of two water images, one saturated onresonance on the lactate resonance and one irradiated on the contralateral frequency. 70 Figure 9.…”

Section: Lactatementioning

confidence: 99%

Magnetization transfer MRS

Leibfritz¹,

Dreher²

2001

NMR in Biomedicine

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This review deals with magnetization transfer (MT) effects observed in in vivo NMR spectroscopy. The basic experimental methods of MT experiments, the underlying kinetic mechanisms as well as the evaluation of measured data by fits to two-or three-pool models are described. Experimental results of both 31 P and 1 H in vivo MRS are reviewed showing the potential of MT experiments to characterize kinetic equilibrium reactions. This includes reactions where all involved components are MR visible, as well as situations where one indirectly measures pools of bound spins which cannot directly be observed in vivo. In particular, MT effects are described which have been observed in in vivo 1 H NMR spectra measured on the animal or human brain or on skeletal muscle. Possible mechanisms for the strong MT effects observed for the signals of creatine/phosphocreatine, lactate, alcohol and other metabolites are discussed. It is also emphasized that MT effects caused by water suppression techniques may lead to systematic errors in the quantification of in vivo 1 H NMR spectra.

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“…The difference spectrum showed significant intensity changes in Glu C2 at 55.7 ppm only. (a) The 1 H results obtained after infusion of [1,[6][7][8][9][10][11][12][13] C 2 ]glucose from the localized spectroscopy voxel (5 × 2.9 × 5 mm 3 ) in rat brain using the INEPT-based inverse 13 C-to-1 H heteronuclear polarization transfer technique. TR = 9.2 sec.…”

Section: Discussionmentioning

confidence: 99%

“…The crowded proton spectrum may also make it difficult to avoid RF spillover effect unless extreme care is exercised [8][9][10]. These factors may explain why magnetization transfer effect of AAT, LDH and MDH reactions has escaped detection although the offset-dependent magnetization transfer effect between tissue water and metabolites has been investigated extensively using proton MRS [11,12].…”

Section: Introductionmentioning

confidence: 99%

Inverse polarization transfer for detecting in vivo 13C magnetization transfer effect of specific enzyme reactions in 1H spectra

Yang

Shen

2008

Magnetic Resonance Imaging

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The wide chemical shift dispersion and long T 1 of 13 C have allowed determination of in vivo magnetization transfer effects caused by aspartate aminotransferase and lactate dehydrogenase reactions using 13 C MRS. In this report, we demonstrate that these effects can be observed in the proton spectra by transferring the equilibrium magnetization of 13 C via the one-bond scalar coupling between 13 C and 1 H using an inverse INEPT-based heteronuclear polarization transfer method. This inverse method allows a combination of the advantages of the long 13 C T 1 for maximum magnetization transfer and the high sensitivity of proton detection. The feasibility of this in vivo inverse polarization transfer approach was evaluated for detecting the 13 C magnetization transfer effect of aspartate aminotransferase and lactate dehydrogenase reactions from a 72.5 μL voxel in the rat brain at 11.7 Tesla.

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Protein Mediated Magnetic Coupling between Lactate and Water Protons

Cited by 34 publications

References 27 publications

In vivo ¹³C saturation transfer effect of the lactate dehydrogenase reaction

In vivo ¹³C saturation transfer effect of the lactate dehydrogenase reaction

Magnetization transfer MRS

Inverse polarization transfer for detecting in vivo 13C magnetization transfer effect of specific enzyme reactions in 1H spectra

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Protein Mediated Magnetic Coupling between Lactate and Water Protons

Cited by 34 publications

References 27 publications

In vivo 13C saturation transfer effect of the lactate dehydrogenase reaction

In vivo 13C saturation transfer effect of the lactate dehydrogenase reaction

Magnetization transfer MRS

Inverse polarization transfer for detecting in vivo 13C magnetization transfer effect of specific enzyme reactions in 1H spectra

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Product

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In vivo ¹³C saturation transfer effect of the lactate dehydrogenase reaction

In vivo ¹³C saturation transfer effect of the lactate dehydrogenase reaction