Protein-imprinted materials: rational design, application and challenges

Yang, Kaiguang; Zhang, Lihua; Liang, Zhen; Zhang, Yukui

doi:10.1007/s00216-012-5840-y

Cited by 92 publications

(58 citation statements)

References 93 publications

(131 reference statements)

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“…The difficulty in epitope imprinting however originates from the choice of specific fragments that can be imprinted and are capable of selectively capturing the target [150]. In addition, certain reports suggest that whole-protein imprinting is more efficient as compared to epitope imprinting [151].…”

Section: Discussionmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Discussionmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…Natural products, on the other hand, have evolved to interact with biomolecules, which have been dominating the pharmaceutical industry for a long time, increasingly turning out to be successful. They have contributed to the development of important therapeutics to combat diseases over the past decades (Yang et al, 2012). The value of natural products to drug discovery is still considerable, however, the protein target(s) of action of these fascinating compounds are largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

Zhang

Sun

et al. 2013

OMICS: A Journal of Integrative Biology

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Scoparone is an active ingredient of Yinchenhao (Artemisia annua L.), a well-known Chinese medicinal plant, and has been utilized in prevention and therapy of liver damage. However, the molecular drug targets associated with the pharmacological effects of scoparone are largely unknown. In the present article, we extend the previous research on Yinchenhao through a study of its active ingredient and thus the putative targets of scoparone. We employed two-dimensional gel electrophoresis, and all proteins expressed were identified by MALDI-TOF/TOF MS and database research. Protein-interacting networks and pathways were also mapped and evaluated. The possible protein network associated with scoparone was constructed, and contribution of these proteins to the protective effect of scoparone against the carbon tetrachloride-induced acute liver injury in rats are discussed herein. Hepatoprotective effects of scoparone on liver injury in rats were associated with regulated expression of six proteins which were closely related in our protein-protein interaction network, and appear to be involved in antioxidation and signal transduction, energy production, immunity, metabolism, and chaperoning. These observations collectively provide new insights on the molecular mechanisms of scoparone action against hepatic damage in rats.

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“…In the former case [16,20,21], the binding sites are formed throughout bulk materials, originated from small molecule imprinting, but suffered from poor mass transfer and heterogeneity of recognition sites [22]. In the latter case, binding sites are formed on the material surface [22,23], which gains the popularity because of easy recognition accessibility and template removal.…”

Section: Introductionmentioning

confidence: 99%

“…Grafted imprinting is a novel surface imprinting strategy to immobilize template on the film or particle surface, followed by the polymerization of monomers and cross-linkers to form a state-of-the-art recognition sites [15,23,24]. As a result, effective template utilization and more imprinting sites can be achieved.…”

Section: Introductionmentioning

confidence: 99%

“…Constrained imprinting is achieved by a planar thin film produced by casting process. With polymerization, the template structure is first confined between the matrix and polymer-forming components, followed by the support removal, by either simply peeling imprinted polymer for the matrix or dissolving the matrix [23]. As a result, this approach gain its advantages that the imprinted sites are exposed on the surface after etching and high template utilization can be achieved.…”

Section: Introductionmentioning

confidence: 99%

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Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique

Yang

Liu

et al. 2013

Microchim Acta

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A novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. First, transferrin was immobilized on silica beads by non-covalent absorption. Then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25°C. Finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. The binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. The imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease B, cytochrome c and β-lactoglobulin. The results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. They may be applied as an artificial antibody to remove the high abundance proteins in plasma.

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Protein-imprinted materials: rational design, application and challenges

Cited by 92 publications

References 93 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique

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