Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction

Murata, Masaru; Yano, Kentaro; Kuroki, Shinichiro; Suzutani, Tatsuo; Katayama, Yoshiki

doi:10.2116/analsci.19.1569

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…[15][16][17] We found that ICSs do not require complete dehydration of the analyte ions from the aqueous phase as reported in previous research, 6 which allows the detection of hydrophilic ions more easily. We also found that a larger change in the surface charge upon binding of analytes to receptors generally results in a higher sensitivity in the ICSs.…”

Section: Introductionsupporting

confidence: 78%

Ion-channel Sensors Based on ETH 1001 Ionophore Embedded in Charged-alkanethiol Self-assembled Monolayers on Gold Electrode Surfaces

et al. 2006

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An ion-channel sensor was demonstrated by immobilizing ETH 1001, an ionophore for ion-selective electrodes, on a gold electrode surface. The approach for preparing the sensor was to incorporate the ionophore into a mixed self-assembled monolayer of 10-mercaptodecanesulfonate and 11-hydroxy-1-undecanethiol formed on the surface. The voltammetric responses for the thus prepared sensor to the primary cation Ca 2+ were observed by using [Fe(CN)6] 3-/4-as an electroactive marker. The ionophore was stably immobilized on the electrode surface with the hydrophobic interaction between its alkyl chains and those of the alkanethiol. The introduction of a proper charge density to the electrode surface improved the sensor sensitivity with retaining the selective response to Ca 2+ against Mg 2+ with concentrations above 10 -4 M.

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Section: Introductionsupporting

confidence: 78%

Ion-channel Sensors Based on ETH 1001 Ionophore Embedded in Charged-alkanethiol Self-assembled Monolayers on Gold Electrode Surfaces

et al. 2006

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“…11 Electrochemical immunosensors have also proven to be capable of the simultaneous detection of L-T4, D-T4 and L-T3 molecules. 13 Murata et al 14 have reported an affinitybased biosensor for the detection of hormones as estrogen and THs, in which TR-LBD was immobilized on a thiolmodified gold electrode; the detection response was evaluated by cyclic and differential pulse voltammetries. These techniques showed the specific binding of T3 to the bioreceptor layer, i.e., the receptor immobilized on a gold electrode.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Sensors Containing Immobilized Nuclear Receptors for Thyroid Hormone Detection

Bendo¹,

Figueira²,

Polikarpov³

et al. 2014

j biomed nanotechnol

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Thyroid hormone receptors (TRs) are members of the nuclear receptors (NRs) superfamily, being encoded by two genes: TRa and TRbeta. In this paper, the ligand-binding domain (LBD) of the TRbeta1 isoform was immobilized on the surface of nanostructured electrodes for TR detection. The platforms containing TRbeta1-LBD were applied to the detection of specific ligand agonists, including the natural hormones T3 (triiodothyronine) and T4 (thyroxine), and the synthetic agonists TRIAC (3,5,3'-triiodothyroacetic acid) and GC-1 [3,5-dimethyl-4-(4'-hydroxy-3'-isopropylbenzyl phenoxy) acetic acid]. Detection was performed via impedance spectroscopy. The biosensors were capable of distinguishing between the thyroid hormones T3 and T4, and/or the analogues TRIAC and GC-1 at concentrations as low as 50 nM. The detection and separation of thyroid hormones and analogue ligands by impedance techniques represents an innovative tool in the field of nanomedicine because it allows the design of inexpensive devices for the rapid and real-time detection of distinct ligand/receptor systems.

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“…Katayama et al . also reported immobilization of an estrogen-receptor [10] and transcription factor [11] on a gold electrode using this method.…”

Section: Introductionmentioning

confidence: 99%

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

Isoda

Maeda

2012

JFB

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Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using silica particles with surfaces modified by aminoalkyl chains ([A-AM silane]/SiO2). [A-AM silane]/SiO2 was synthesized by silane coupling reaction of N-2-(aminoethyl)-3-aminopropyltrimethoxysilane (A-AM silane) with SiO2 particles with a diameter of 5 μm at 100 °C for 20 min. The surface chemical structure of [A-AM silane]/SiO2 was characterized by Fourier transform infrared spectroscopy and molecular orbital calculations. The surface of the silica particles was modified with A-AM silane and primary amine groups were formed. [A-AM silane]/SiO2 was trapped with single-stranded nucleic acids [(Poly-X; X = A (adenine), G (guanine) and C (cytosine)] in PBS solution at 37 °C for 1 h. The single-stranded nucleic acids were trapped on the surface of the [A-AM silane]/SiO2 by hydrogen bonding to form conjugated materials. The resulting complexes were further conjugated by derivatives of acridine orange (AO) as fluorescent labels under the same conditions to form [AO:Poly-X:A-AM silane]/SiO2 complexes. Changes in the fluorescence intensity of these complexes originating from interactions between the single-stranded nucleic acid and aromatic compounds were also evaluated. The change in intensity displayed the order [AO: Poly-G: A-AM silane]/SiO2 > [AO:Poly-A:A-AM silane]/SiO2 >> [AO:Poly-C:A-AM silane]/SiO2. This suggests that the single-stranded nucleic acids conjugated with aminoalkyl chains on the surfaces of SiO2 particles and the change in fluorescence intensity reflected the molecular interaction between AO and the nucleic-acid base in a polynucleotide.

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Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction

Cited by 9 publications

References 20 publications

Ion-channel Sensors Based on ETH 1001 Ionophore Embedded in Charged-alkanethiol Self-assembled Monolayers on Gold Electrode Surfaces

Ion-channel Sensors Based on ETH 1001 Ionophore Embedded in Charged-alkanethiol Self-assembled Monolayers on Gold Electrode Surfaces

Nanostructured Sensors Containing Immobilized Nuclear Receptors for Thyroid Hormone Detection

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

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