Protein hydrolysates and ultrafiltered &lt; 1 KDa fractions from <scp>Phaseolus lunatus</scp>, Phaseolus vulgaris and <scp>Mucuna pruriens</scp> exhibit antihyperglycemic activity, intestinal glucose absorption and α‐glucosidase inhibition with no acute toxicity in rodents

Núñez-Aragón, Pablo Noé; Campos, Maira Rubí Segura; Negrete-León, Elizabeth; Acevedo-Fernández, Juan José; Betancur‐Ancona, David; Chel‐Guerrero, Luis; Castañeda-Corral, Gabriela

doi:10.1002/jsfa.9219

Cited by 25 publications

(15 citation statements)

References 31 publications

(87 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Weight loss can be explained as independent of food intake: Fat reserves of HFD mice could have been mobilized due to reduced total energy in form of glucose, whose plasmatic level was lowered under treatment [17,33,34]. Some decades after its discovery, the alpha amylase inhibitor-1 phaseolamin was tested for anti-obesity starch-blocker effects on humans: Even if a significant weight loss in obese and healthy subjects treated with the commercial αAI1 extract Phase 2 TM was reported, in other trials different commercial phaseolamin-based starch-blockers did not influence body weight.…”

Section: Discussionmentioning

confidence: 99%

“…The kidney extract was protective against hyperglycaemia, a result consistent with many studies on rats (diabetic or healthy, fed with normal or starch-enriched diet), where different quantities of the α-amylase inhibitor, chronically administrated from 10 to 20 days, induced a significant reduction of glucose blood levels at the end of the prolonged treatment. In addition, when acutely administrated, this lowering effect was observed on the post-prandial glycaemia levels, even if not always statistically significant [17,33,34]. The efficacy on post-prandial hyperglycaemia was confirmed in humans by a study of Spadafranca and her collaborators on healthy men and women, who were given a tablet of P. vulgaris extract (containing both α-AI and phytohaemagglutinin; the same used in the present study) to be ingested before a standardized meal [37].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Phaseolus vulgaris L. Extract: Alpha-Amylase Inhibition against Metabolic Syndrome in Mice

et al. 2019

View full text Add to dashboard Cite

To examine the effects of the alpha-amylase inhibitor isoform 1 called phaseolamin, a standardized extract from white kidney beans (Phaseolus vulgaris L) was tested against the hallmarks of metabolic syndrome. The efficacy of a per os repeated treatment with P. vulgaris extract (500 mg/kg) was compared with metformin (100 mg/kg) and atorvastatin (10 mg/kg) in a model of metabolic syndrome evoked by prolonged high fat diet (HFD; week 1 to week 19) in C57BL/6 mice. Bean extract and compounds administration started after metabolic syndrome establishment (week 11). P. vulgaris extract reduced the body weight overtime, as well as effectively lowered glycaemia, triglycerides, and cholesterol. On week 19, bean extract normalized the HFD-evoked tolerance to glucose and insulin. According to the phytochemical characterization, it inhibited the alpha-amylase activity. Animals treated with the extract were rescued from motor impairments and nociceptive threshold alterations induced by HFD. Specific organs analysis revealed that P. vulgaris extract decreased hepatic steatosis and lipid peroxidation in liver. It protected the heart from HFD oxidative alterations increasing the expression of the detoxifying enzymes catalase and glutathione reductase, and normalizing NADH dehydrogenase level. The histological analysis of aorta showed a protection about the development of fatty streaks in the muscular layers. In conclusion, a prolonged treatment with the standardized extract of P. vulgaris significantly reduced several pathological features related to a metabolic syndrome-like condition; a multifactorial approach that candidates this vegetal product as a possible therapeutic option against metabolic syndrome.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phaseolus vulgaris L. Extract: Alpha-Amylase Inhibition against Metabolic Syndrome in Mice

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Bioactive peptide extraction was reported using different proteases hydrolysis of protein, and different peptide fractions were obtained by ultrafiltration membranes with different cutoff molecular weights, while the α-amylase inhibition rate was also determined . [29] These methods are summarized in Table 1 separation section.…”

Section: Separation and Purification Of Proteinaceous α-Amylase Inhibitorsmentioning

confidence: 99%

“…The results showed that protein hydrolyzate with a molecular weight below 1 kDa inhibited αamylase activity and reduced glucose uptake in the small intestine . [29] At present, measurements of α-amylase inhibitory activity in vitro are quite mature, and based mainly on the quantification of amylase activity, so that the activity of the inhibitors can be obtained indirectly. However, amylase activity is affected by many factors that are more complex in vivo than those in vitro, so the development of a more targeted in vivo assay method is essential.…”

Section: Vivo Experimentsmentioning

confidence: 99%

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Zhou

Zhang

et al. 2021

International Journal of Food Properties

View full text Add to dashboard Cite

α-Amylase is abundant in plants and animals. α-Amylase inhibitors can reduce endogenous α-amylase activity, playing an essential role in agricultural pest control, and preventing and treating human disease. In the agricultural field, α-Amylase inhibitors can restrict pest that relies on the starch of crops. Acarbose is an α-amylase inhibitor used to treat diabetes. Some αamylase inhibitors are represented by antinutritional factors, while others are proteinaceous. Depending on their structures and sources, researchers have divided them into seven types: The knottin-like type, the γ-thionin-like type, the cereal type, the Kunitz type, the thaumatin-like type, and the lectin-like type. This paper introduces the methods for separating, purifying, and detecting proteinaceous α-amylase inhibitors while examining the structure and inhibition mechanism of several proteinaceous α-amylase inhibitors. Finally, it explores the potential applications of α-amylase inhibitors.

show abstract

“…Antihyperglycemic activity was measured by OSTT tests in healthy and HSD Wistar rats (n = 5), as follows: (i) a positive control using starch and acarbose (0.5 mg kg −1 ); (ii) a negative control using only starch; (iii) treatment groups using starch and different extracts of Mayan plant foods at concentrations of 5 and 10 mg kg −1 . 17 To determine the doses for this study, all Mayan plant food extracts were tested at 5 mg kg −1 as initial dose screening. These results compared to the negative control group were used to determine a second dose (10 mg kg −1 ).…”

Section: Antihyperglycemic Activitymentioning

confidence: 99%

Antihyperglycemic and hypoglycemic activity of Mayan plant foods in rodent models

et al. 2021

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Postprandial hyperglycemia and decreased insulin secretion are relevant to risk factors in the development of type 2 diabetes and its complications. Plant foods with antidiabetic properties could be an affordable alternative in the prevention and treatment of this disease. In the present study, the antihyperglycemic and hypoglycemic activity of Bixa orellana, Psidium guajava L., Cucurbita moschata, Raphanus sativus L. and Brassica oleracea var. capitata -Mayan plant foodswere evaluated at doses of 5 and 10 mg kg −1 . Antihyperglycemic activity was measured in healthy Wistar rats and those with obesity induced by high-sucrose diet (group HSD) (20%). The hypoglycemic activity was measure in healthy CD1 mice. RESULTS: Fasting glucose, Lee index and the body weight of HSD rats increased significantly (P ≤ 0.05) after 12 weeks of induction compared to healthy rats. In healthy rats, P. guajava and Bixa orellana (10 mg kg −1 ) demonstrated higher and statistically different (P ≤ 0.05) antihyperglycemic activity compared to control acarbose (0.5 mg kg −1 ). In the HSD rat group, all Mayan plant foods (10 mg kg −1 ) demonstrated antihyperglycemic activity statistically equal (P ≤ 0.05) to control acarbose. However, Brassica oleracea and R. sativus registered the highest antihyperglycemic activity. Bixa orellana and P. guajava (5 mg kg −1 ) showed similar hypoglycemic activity (P ≤ 0.05) to glibenclamide (0.5 mg kg −1 ) but was not significant (P ≤ 0.05) compared to insulin (5 UI kg −1 ). CONCLUSION:The present study provides valuable evidence on the possible health benefits of Mayan plant foods. These foods could contribute to the development of therapeutic diet strategies for the prevention and treatment of diabetes.

show abstract

Protein hydrolysates and ultrafiltered < 1 KDa fractions from Phaseolus lunatus, Phaseolus vulgaris and Mucuna pruriens exhibit antihyperglycemic activity, intestinal glucose absorption and α‐glucosidase inhibition with no acute toxicity in rodents

Abstract: These results suggest that hydrolysates and < 1 kDa fractions from P. lunatus, P. vulgaris and M. pruriens are suitable candidates to treat or prevent T2DM. © 2018 Society of Chemical Industry.

Cited by 25 publications

References 31 publications

Phaseolus vulgaris L. Extract: Alpha-Amylase Inhibition against Metabolic Syndrome in Mice

Phaseolus vulgaris L. Extract: Alpha-Amylase Inhibition against Metabolic Syndrome in Mice

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Antihyperglycemic and hypoglycemic activity of Mayan plant foods in rodent models

Contact Info

Product

Resources

About