Protein Haemaglutinin Outer Membran Protein (OMP) 35 kDa sebagai Protein Adhesin Proteus mirabilis pada Vesika Urinaria Kelinci

Abstract

Proteus mirabilis is opportunistic and nosocomial pathogen that usually found in clinical specimen from patients with catheter. The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity of the protein as hemaglutinin and adhesion molecule. The aim of this study is to evaluate the role of 35 kDa outer membrane protein from P. mirabilis. After identification, bacterial isolate of OMP fraction 12,5% SDS-PAGE were used to isolate OMP followed by hemaglutinin test and invitro adhesion test. The study showed that the 35 kDa OMP of P. mirabilis was a hemaglutinin protein that could agglutinate mice erythrocytes, rabbit erythrocytes, and human group O erythrocytes. Hemaglutination test were negative on erythrocytes human blood group A,B, and AB. The 35 kDa OMP was also adhesion protein showed by its activity to adhere to the rabbit vesica urinaria epithel receptor. The increase dose of 35 kDa OMP will decrease the amount of P. mirabilis bacteria to adhere to rabbit vesica urinaria epithel (p< 0,05 ).