Protein Fractions of the Blood Plasma and Dental-Pulp Fluid of the Dog

Abstract

The concentration of protein in the dental-pulp fluid of the dog has been found to be approximately one-fifth of that in the blood plasma.' The present study was undertaken to determine the relative concentration of protein fractions in these two body fluids.Materials and Methods After the dog had been completely anesthetized with nembutal, a hole was drilled with an air-turbine handpiecet in the manner described elsewhere' through the buccal enamel and dentin to the pulp chamber in the maxillary and mandibular canine, premolar, and first molar teeth on the same side of the jaw. A capillary glass tube was inserted in each hole and sealed in place. After several hours, clear, colorless fluid, which we have designated as dental-pulp fluid, had collected in the tubes in sufficient amount for the desired analyses. Plasma was obtained from venous blood drawn toward the end of the experiment.In numerous experiments in which this technique was employed, we could detect, upon microscopic examination, no erythrocytes or leukocytes in the dental-pulp fluid. The chairman of the Department of Oral Pathology at this institution made and examined for us histologic decalcified sections of the teeth in some of these experiments. He could find no evidence of pulpal damage. He saw no indication of inflammatory response in the pulp at the levels where the holes had been drilled. We have therefore concluded that the dental-pulp fluid is a capillary transudate.Separation of the protein fractions was effected in an electrophoresis instrument,: using filter-paper strips § and sodium barbitone-sodium acetate buffer of pH 8.6 and 0.1 ionic strength. In preliminary trials it was found that the concentration of the protein fractions in the pulp fluid was so low as to give only faint, indistinct markings on the filter-paper strips when subjected to electrophoresis. The pulp fluid was therefore freeze-dried and then made up with distilled water to a volume that was five times as concentrated as the original.

Comparative Concentration of Various Constituents of Blood Plasma and Dental-Pulp Fluid

“…In the light of the results in the present experiments taken in conjunction with those in previous studies," 20,21 we are now of the opinion that the pulp fluid is a capillary filtrate. As the average concentrations of the blood constituents found in the pulp fluid were never significantly higher than in the blood plasma, filtration and perhaps also diffusion would appear to account for the production of the pulp fluid.…”

Classification of Ancient Mammal Individuals Using Dental Pulp MALDI-TOF MS Peptide Profiling

“…Peptide sequencing confirmed the detection of collagen in both modern and ancient dental pulp specimens, indicating that the dental pulp is a previously uninvestigated source for this protein which is widely used for the classification of ancient mammal individuals at the species level, in addition to bone tissue [7], [24]–[27]. Moreover, peptide sequencing still proved the detection of 2 additional proteins in ancient dental pulp specimens, which have been previously reported as present in the human dental pulp [23], [28], [29]. Sequencing therefore indicated that peptide profiling identification did not rely on only one protein and that the dental pulp is a suitable source of non-collagen proteins.…”

Effectiveness and cytotoxicity of two desensitizing agents: a dentin permeability measurement and dentin barrier testing in vitro study

“…When the dentin is exposed, the outflowing dentinal fluid contains a fraction of plasma proteins [ 30 ]. Albumin is the main protein component in the plasma and dentinal fluid [ 38 ]. According to the protein fraction in the plasma and dentinal fluid, 1:3 diluted bovine serum was used to simulate the dentinal fluid in some studies [ 28 , 38 ], although Özok et al suggested that it was not a realistic substitute for dentinal fluid because of the relatively large molecular weight of the substances in the serum fraction [ 30 ].…”

“…Albumin is the main protein component in the plasma and dentinal fluid [ 38 ]. According to the protein fraction in the plasma and dentinal fluid, 1:3 diluted bovine serum was used to simulate the dentinal fluid in some studies [ 28 , 38 ], although Özok et al suggested that it was not a realistic substitute for dentinal fluid because of the relatively large molecular weight of the substances in the serum fraction [ 30 ]. Substances in the serum components with a molecular weight > 100,000 Da, such as globulins and lipoproteins, can reduce dentin permeability [ 30 ].…”