Protein fractionation of Moringa oleifera Lam. seeds and functionalization with magnetic particles for the treatment of reactive black 5 solution

Abstract

Industrial effluents are sewage from industrial processes, such as polluted cooling waters and waters used for cleaning. It is extremely important that these contaminated waters undergo treatment to allow for water reuse or safe disposal, without damage to the environment. In the present work, the efficacy of coagulating/flocculating aqueous solutions containing reactive black 5 (RB5) dye was evaluated using protein fractions derived from Moringa oleifera seeds (albumin and globulin) as natural coagulants functionalized with iron oxide magnetic nanoparticles. The application of a magnetic field during sedimentation allowed for faster settlement of the dye particles and their separation. Variations in parameters such as the protein concentration, nanoparticle concentration, solution pH, dye concentration, and the presence or absence of an alkalizing agent were assessed. Moreover, the sedimentation time was analyzed with and without the magnetic field and with the reuse of iron oxide nanoparticles. The functionalized magnetic coagulants were able to remove more than 90 % of RB5 dye within 5 min of sedimentation. Figure 11. Influence of the presence of an alkalizing agent (concentration of AlbFe (a): 115 mg L À1 ; concentration of GloFe (b): 200 mg L À1 ; pH: 6; and dye concentration: 20 mg L À1 ).

Water quality restoration by harvesting mixed culture microalgae using Moringa oleifera

“…The two strong absorption bands at 1,656 and 1,542 cm −1 usually signify amide I and II, respectively, which are in the usual range of absorption of helices of proteins, confirming the presence of proteins in the coagulating active materials (Kwaambwa & Maikokera, 2008). The FTIR results of the present study are in conjunction with the study by Baptista et al (2017) and Reck et al (2019). They fractionalized the coagulating protein of MO seeds into albumin and globulin for conducting coagulation.…”

“…The large bands in the region of 3,200-3,500 cm −1 may be attributed to the presence of hydroxyl groups (OH) that might be present in proteins, fatty acids, carbohydrates, and lignin (Baptista et al, 2017). The bands present at 2,923 and 2,852 cm −1 signify the C-H-CH 2 group that is present in fatty acids (Reck et al, 2019). The two strong absorption bands at 1,656 and 1,542 cm −1 usually signify amide I and II, respectively, which are in the usual range of absorption of helices of proteins, confirming the presence of proteins in the coagulating active materials (Kwaambwa & Maikokera, 2008).…”

Experimental methods in chemical engineering: Zeta potential

“…journal published 16 articles that mention the technique. [ 43,48,50–63 ] The hot topics in the journal relate to the oil industry including asphaltene adsorption on calcite packs, [ 56 ] improved heavy oil recovery by injecting alkaline ionic slugs (blue cluster), [ 54 ] and rheological properties for composite fluids for fracturing and in mineral processing (green cluster). [ 58,61 ] Other topics relate to nanoparticles in polymers (red cluster) and bioimaging, [ 43,48 ] drug delivery (red cluster), [ 50 ] and environmental remediation (yellow cluster).…”

Discolouration of contaminated water with textile dye through a combined coagulation/flocculation and membrane separation process with different natural coagulants extracted from Moringa oleifera Lam. seeds

“…[ 5,6 ] Thus, the treatment of dye‐containing wastewaters has become a major challenge, especially for reactive and anionic dyes, such as the reactive black 5 dye (RB5), used to colour cotton fibres, because ~30% of these dyes are wasted due to hydrolysis of the dye in alkaline in bath. [ 7,8 ]…”