Protein Fibre Surface Modification

Dyer, J. W. W.; Grosvenor, Anita J.

doi:10.5772/22601

Cited by 8 publications

(7 citation statements)

References 53 publications

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“…Human hair by nature is a fibrous material with good tensile properties. The primary component of the hair fiber is keratin which consists of proteins and long chain (polymers) of amino acids 3 . Cortex in the hair fiber is primarily responsible for the tensile properties of human hair 4 .…”

Section: Introductionmentioning

confidence: 99%

Tensile Studies on Random Oriented Human Hair Fiber Reinforced Polyester Composites

Rao¹,

Kiran²,

Prasad³

2018

J. mech. eng.

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Section: Introductionmentioning

confidence: 99%

Tensile Studies on Random Oriented Human Hair Fiber Reinforced Polyester Composites

Rao¹,

Kiran²,

Prasad³

2018

J. mech. eng.

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“…This l-Cys fall is understandable, in that recently new approaches relying on removal of lipids from the surface of fibers have exposed their surface with a variety of reactive moieties. In this sense, covalent treatments can be directed straightforward to these reactive sites, rather than physically-directed, which offers higher durability and resistance to laundry [25].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and antioxidant surface modification toward a new silk-fibroin (SF)-l-Cysteine material for skin disease management

Nogueira

Granadeiro

Mouro

et al. 2016

Applied Surface Science

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A novel dressing material-silk fibroin fabric (SF)-l-Cysteine (l-Cys)-is here developed to be used as standard treatment for atopic dermatitis (AD), which combines comfort, thermic, and tensile strength properties of silk materials with antioxidant and antimicrobial effects of l-Cys. A careful understanding about the linking strategies is needed in order not to compromise the bioavailability of l-Cys and deplenish its bioactivity. Durability was also addressed through washing cycles and compared with hospital requirements, according to international Standard EN ISO 105-C06:2010. The present research also analyze the interactions between Staphylococcus aureus and SF-l-Cys under simulating conditions of AD and demonstrated the effectiveness of a double covalent grafting, with the importance of SF tyrosine (Tyr) covalent linkage with l-Cys (SF-g-l-Cys/Tyr-g-l-Cys) even after several washing cycles, twenty five, whereas for a disposable application a single covalent mechanism of grafting l-Cys proved to be sufficient (SF-g-l-Cys). Results showed effective antimicrobial activities exhibiting higher inhibition ratios of 98.65% for SF-g-l-Cys after 5 washing cycles, whereas 97.55% for SF-g-l-Cys/Tyr-g-l-Cys after 25 washing cycles, both at pH 9.5 grafting strategy. Furthermore, it is also reported a non-protumoral effect of l-Cys. A new advance is herein achieved at the world of medical antimicrobial textiles tailored to address wound moisture environment and exudate self-cleaning, which may open novel applications as complementary therapy for AD disease.

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“…The latter is applied for the treatment of heat-sensitive objects such as polymeric materials and biological samples without introducing damages. During the process, gas particles such as ions, electrons, radicals, neutrals, and UV radiation etch the surface in the nanoscale and lead to modified functional properties of the matrix [6]. Recek et al (2013) have used this characteristic of plasma to study protein adhesion and cell response to plasma-treated polymer surfaces, such as absorption of albumin protein on the modified surfaces [7].…”

Section: Introductionmentioning

confidence: 99%

Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus

Ehsani

Fahmide

Norouzian

et al. 2019

JoMMID

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Introduction: Staphylococcus aureus is a source of nosocomial infections and one of the significant concerns in patients with indwelling devices. Lysostaphin is a bacterially produced endopeptidase with a unique activity on S. aureus. Plasma, the fourth state of the material, consists of charged ions, free electrons, and activated neutral species. Biomedical applications of cold plasma are rapidly growing due to its capacity to treat heat-sensitive objects such as polymeric materials and biological samples. It activates surfaces by etching them to stabilize proteins. The direct effect of cold atmospheric plasma on the eradication of microorganisms have been investigated. However, there is no report on immobilizing antibiotic agents. Methods: In this study, the lysostaphin protein was expressed and purified using Ni-NTA column, then the purified enzyme was immobilized on glass surfaces pretreated with cold atmospheric plasma for 150 s, 200 s, and 300 s. The antimicrobial activity of immobilized lysostaphin on S. aureus was approved by in vitro analysis. Results: The 300 s plasma treatment confirmed to be the best time arrangement for more lysostaphin immobilization, shown by Atomic Force Microscopy. Conclusion: Our results showed that passive adsorption to the treated surface does not affect the structure and subsequent antimicrobial function of the recombinant protein compared to the standard proteins.

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Protein Fibre Surface Modification

Cited by 8 publications

References 53 publications

Tensile Studies on Random Oriented Human Hair Fiber Reinforced Polyester Composites

Tensile Studies on Random Oriented Human Hair Fiber Reinforced Polyester Composites

Antimicrobial and antioxidant surface modification toward a new silk-fibroin (SF)-l-Cysteine material for skin disease management

Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus

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