Protein engineering approaches for enhanced catalytic efficiency

Zhang, Guoqiang; Chen, Yukun; Du, Guocheng

doi:10.1016/b978-0-12-819820-9.00007-7

Cited by 4 publications

(2 citation statements)

References 96 publications

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“…4 These phases have become indispensable in harnessing the immense potential of enzyme biocatalysis, enabling pharmaceutical scientists to judiciously engineer enzymes, fabricate novel biocatalysts, and systematically refine their performance. 95 In this manner, they drive innovation and enhance efficiency in both drug discovery and production.…”

Section: Key Factors Enabling Enzyme Biocatalysis In Pharmaceutical A...mentioning

confidence: 99%

Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

Kim,

Ga,

Bae

et al. 2024

EES. Catal.

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Section: Key Factors Enabling Enzyme Biocatalysis In Pharmaceutical A...mentioning

confidence: 99%

Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

Kim,

Ga,

Bae

et al. 2024

EES. Catal.

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“…Traditionally, the rational design concept is applied to in silico design of new biomaterials and/or nanomaterials, followed by experimental verification using various techniques [ 80 ]. Some argue that directed evolution approaches, or combinations of these two approaches, are advantageous with respect to classic protein engineering [ 81 , 82 , 83 , 84 ]. Both of these frameworks have been extremely powerful for protein engineering and have also advanced the field of biosensor development.…”

Section: A Framework For Connecting Biomolecular Interaction Paramete...mentioning

confidence: 99%

An Experimental Framework for Developing Point-of-Need Biosensors: Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy

et al. 2022

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Biolayer interferometry (BLI) is a well-established laboratory technique for studying biomolecular interactions important for applications such as drug development. Currently, there are interesting opportunities for expanding the use of BLI in other fields, including the development of rapid diagnostic tools. To date, there are no detailed frameworks for implementing BLI in target-recognition studies that are pivotal for developing point-of-need biosensors. Here, we attempt to bridge these domains by providing a framework that connects output(s) of molecular interaction studies with key performance indicators used in the development of point-of-need biosensors. First, we briefly review the governing theory for protein-ligand interactions, and we then summarize the approach for real-time kinetic quantification using various techniques. The 2020 PRISMA guideline was used for all governing theory reviews and meta-analyses. Using the information from the meta-analysis, we introduce an experimental framework for connecting outcomes from BLI experiments (KD, kon, koff) with electrochemical (capacitive) biosensor design. As a first step in the development of a larger framework, we specifically focus on mapping BLI outcomes to five biosensor key performance indicators (sensitivity, selectivity, response time, hysteresis, operating range). The applicability of our framework was demonstrated in a study of case based on published literature related to SARS-CoV-2 spike protein to show the development of a capacitive biosensor based on truncated angiotensin-converting enzyme 2 (ACE2) as the receptor. The case study focuses on non-specific binding and selectivity as research goals. The proposed framework proved to be an important first step toward modeling/simulation efforts that map molecular interactions to sensor design.

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Microbial Peptidase in Food Processing: Current State of the Art and Future Trends

et al. 2022

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Protein engineering approaches for enhanced catalytic efficiency

Cited by 4 publications

References 96 publications

Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

An Experimental Framework for Developing Point-of-Need Biosensors: Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy

Microbial Peptidase in Food Processing: Current State of the Art and Future Trends

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