Protein encapsulation in SBA-15 with expanded pores

Garcia, Paulo R. A. F.; Bicev, Renata N.; Oliveira, Cristiano L. P.; Sant’Anna, Osvaldo A.; Fantini, M.C.A.

doi:10.1016/j.micromeso.2016.07.033

Cited by 26 publications

(41 citation statements)

References 36 publications

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“…Our previous works explained in detail how the complementary techniques, such as NAI, scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), synchrotron radiation circular dichroism (SRCD) and intrinsic tryptophan fluorescence spectroscopies, were used to further evaluate the effectiveness of our strategies to develop oral vaccines, including biological assays. Thus, these results will not be detailed here, we refer the reader to the literature (Carvalho et topical reviews , 2010;Mariano-Neto et al, 2014;Scaramuzzi et al, 2016;Garcia et al, 2016;Lopes et al, 2019;Mercuri et al, 2006;Rasmussen et al, 2019bRasmussen et al, , 2021. These applications will be described and correlated in the Discussion.…”

Section: Materials Characterizationmentioning

confidence: 99%

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Using crystallography tools to improve vaccine formulations

Fantini

Oliveira

Lopes

et al. 2021

Int Union Crystallogr J

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This article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer's patches in the small intestine to be reached and consequently enhancing immunity. Focusing on the pioneering research conducted at the Butantan Institute in Brazil, the optimization of the antigen encapsulation yield is reported, as well as their distribution inside the meso- and macroporous network of the porous silica. As the development of vaccines requires proper inclusion of antigens in the antibody cells, X-ray crystallography is one of the most commonly used techniques to unveil the structure of antibody-combining sites with protein antigens. Thus structural characterization and modelling of pure antigen structures, showing different dimensions, as well as their complexes, such as silica with encapsulated hepatitis B virus-like particles and diphtheria anatoxin, were performed using small-angle X-ray scattering, X-ray absorption spectroscopy, X-ray phase contrast tomography, and neutron and X-ray imaging. By combining crystallography with dynamic light scattering and transmission electron microscopy, a clearer picture of the proposed vaccine complexes is shown. Additionally, the stability of the immunogenic complex at different pH values and temperatures was checked and the efficacy of the proposed oral immunogenic complex was demonstrated. The latter was obtained by comparing the antibodies in mice with variable high and low antibody responses.

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Section: Materials Characterizationmentioning

confidence: 99%

“…The SAXS scattering intensity results I(q) were simulated according to the literature (Mariano-Neto et al, 2014;Garcia et al, 2016), given by…”

Section: Samplementioning

confidence: 99%

Using crystallography tools to improve vaccine formulations

Fantini

Oliveira

Lopes

et al. 2021

Int Union Crystallogr J

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“…203 Relatively few other periodically structured porous hosts or supports can accommodate the intermediate size range that is characteristic of enzymestypically 3-7 nm; 204 SBA-15 and MCM-41 type silicates and aluminosilicates are non-MOF examples. 205,206 The 3 to 7 nm size is greater than what is needed for materials optimized to handle conventional molecules, but smaller than what is needed for materials geared toward interference-based optical sensing applications. Thus far, the broadest benefit of sizematched MOF encapsulation of enzymes has proven to be physical inhibition of denaturation, even when the driving force for denaturation is thermal, chemical (e.g., ureatriggered unfolding), or dehydration.…”

Section: Enzymesmentioning

confidence: 99%

MOF-enabled confinement and related effects for chemical catalyst presentation and utilization

et al. 2022

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“…The final product was kept aging for 24 h at room temperature. The pore-expanded SBA-15 (PE-SBA-15) was prepared in the same way as the SBA-15 with the single exception that the swelling agent 1,3,5-trimethylbenzene (TMB) was added to the synthesis solution prior to the addition of the silatrane complex, with the function of working as a micelle expander [28]. The final solution was kept aging at 70°C for 24 h. The employed molar ratios were: 0.017 P123: 0.0054 TMB: 4.35 HCl: 183 H 2 O: 1 TEOS.…”

Section: Sba-15 and Pore-expanded Pe-sba-15 Synthesismentioning

confidence: 99%

Silica with 3D Mesocellular Pore Structure Used as Support for Cobalt Fischer-Tropsch Catalyst

Pardo-Tarifa¹,

Sánchez²,

Claure³

et al. 2017

Synth Catal

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The typical MCM-41 and SBA-15 silica types were modified in order to expand their pores by addition of a swelling agent at mild conditions. The MCM-41, SBA-15 and Pore-expanded (PE) mesoporous silicas, i.e., PE-SBA-15 and PE-MCM-41 were synthesized by the atrane route and used as supports in the preparation of cobalt Fischer-Tropsch catalysts. The synthetized silicas presented a homogeneous morphology with narrow pore size distribution of 1D, 2D and 3D pore structures. The most interesting pore structure and size was presented by the PE-SBA-15 silica, which showed a 3D spherical cell structure of 30 nm diameter interconnected by small window pores of 7 nm of diameter. These supports were used in cobalt catalyst preparation. The characterization results showed that the pore structure and pore size affected the growth of cobalt oxide particles and cobalt-silica interaction. These factors played an important role on the cobalt dispersion and Degree of Reduction (DOR). Co/SBA-15 and Co/PE-SBA-15 catalysts presented similar physicochemical properties related with the Co 3 O 4 , but the pore size and structure of the support was different. All the catalysts were tested in Fischer-Tropsch reaction at close to industrial conditions. Co/PE-SBA-15 catalyst showed the best CO conversion and selectivity to long-chain hydrocarbons, results attributed to the higher degree of reduction of cobalt but mainly to the open network with 3D structure pores of the silica support which reduced the diffusion limitations of the reactants and products.

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Protein encapsulation in SBA-15 with expanded pores

Cited by 26 publications

References 36 publications

Using crystallography tools to improve vaccine formulations

Using crystallography tools to improve vaccine formulations

MOF-enabled confinement and related effects for chemical catalyst presentation and utilization

Silica with 3D Mesocellular Pore Structure Used as Support for Cobalt Fischer-Tropsch Catalyst

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