Protein detection based on rolling circle amplification sensors

Abstract

Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large-scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection.Sensors based on RCA have attracted increasing interest. Advances in RCA-based sensors for protein detection are reviewed in this paper. The advantages and detection mechanisms of sensors based on RCA are revealed and discussed. Finally, possible challenges and future perspectives are also outlined.

Functional nucleic acid biosensors utilizing rolling circle amplification

“…RCA is an alternative ITA method that has received significant attention for biosensing applications, as described in recent reviews. [84][85][86][87][88][89][90] Replication of DNA around a circular DNA template (CDT) is a naturally occurring process which typically involves circular DNA plasmids that are thousands of nucleotides in length. 91 However, the CDTs used in RCA are often synthesized to be less than one hundred nucleotides long instead.…”

“…99,[101][102][103] Several recent reviews have also covered specific aspects of FNAcoupled RCA for detection of non-NA targets, including development of optical biosensors, 87,89 assays for microorganisms, 88 or assays for protein detection. 90 However, none of these comprehensively cover both DNA aptamer-and DNAzymecoupled RCA systems or discuss examples from the viewpoint of relevance for point-of-care diagnostics, which is a key focus of this review.…”

A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the rpsL Gene Mutation Assay

“…In addition, RCA can amplify a single molecule binding event a thousand-fold, making it suitable for the detection of ultralow abundance targets [24]. Currently, RCA, as a general signal amplification tool, has a wide range of applications in genomics, proteomics, diagnostics, and biosensing [25,26].…”

Ultrasensitive detection of CA125 based on a triple signal amplification strategy with a huge number of loaded probes via exonuclease cyclic cleavage, rolling cyclic amplification and strand self-growth

“…28 These methods involve primary amplification, which usually uses dsDNA loaded signal probes for target detection. 29,30 However, due to the small number of carriers produced by the primary amplification of RCA and EASA, the amount of loaded probes is insufficient, resulting in the signal when detecting the target not being strong enough, 31,32 which limits the sensitivity of the detection. It makes early diagnosis of cancer and monitoring during the treatment period difficult.…”