Functional nucleic acid biosensors utilizing rolling circle amplification

Abstract

Functional nucleic acids regulate rolling circle amplification to produce multiple detection outputs suitable for the development of point-of-care diagnostic devices.

Impact of Divalent Metal Ions on Regulation of Trans‐Cleavage Activity of CRISPR‐Cas13a: A Combined Experimental and Computational Study

“…This phenomenon has also been observed for other Cas protein [28] . For future work, we expect further improvements in the analytical performance of RNA sensor with LbuCas13a or crRNA engineering, [29] and additional integration of the nucleic acid‐based signal amplification methods [30] . Another limitation of our sensors is their ability to detect only one target at a time using the current CRISPR sensor.…”

“…[28] For future work, we expect further improvements in the analytical performance of RNA sensor with LbuCas13a or crRNA engineering, [29] and additional integration of the nucleic acid-based signal amplification methods. [30] Another limitation of our sensors is their ability to detect only one target at a time using the current CRISPR sensor. To expand its potential applications across various fields, future efforts could concentrate on devising a multiplexed CRISPR sensor that can detect multiple targets simultaneously.…”

Detection of Large Genomic RNA via DNAzyme‐Mediated RNA Cleavage and Rolling Circle Amplification: SARS‐CoV‐2 as a Model

“…The resulting long, concatenated reaction products (RPs) can be detected with high sensitivity using many methods, including fluorometrically (using fluorophore-conjugated probes or intercalating dyes) or colorimetrically (using gold nanoparticle hybridization). [19][20][21][22] RCA assays have been widely used for detection of short RNA sequences, such as microRNA targets, as these can directly bind to complementary CDTs to initiate amplification and generate sensitive output signals. [23][24][25] However, direct detection of large genomic RNA by RCA has yet to be demonstrated.…”

A fully integrated nucleic acid analysis system for multiplex detection of genetic polymorphisms related to folic acid metabolism

“…Moreover, the chip's material must have a low fluorescence background to achieve effective detection. 20 In comparison, some isothermal amplification techniques, 21 such as loop-mediated isothermal amplification (LAMP), 22 nucleic acid sequence-based amplification (NASBA), 23 and rolling circle amplification (RCA), 24 only require a constant reaction temperature with a simple control system that is appropriate for point of care testing (POCT). Among these methods, LAMP shows high sensitivity and specificity, but is rarely used for detecting SNPs due to the complex primer design.…”