Protein Corona-Enabled Systemic Delivery and Targeting of Nanoparticles

Chen, Dongyu; Ganesh, Shanthi; Wang, Weimin; Amiji, Mansoor M.

doi:10.1208/s12248-020-00464-x

Cited by 49 publications

(32 citation statements)

References 96 publications

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“…However, successful in vivo outcomes of the use of GNPs are strongly dependent on the interactions between the protein corona layer and the surrounding cells [31,32]. Understanding GNP-protein interactions is crucial for the development, manufacturing, and translation of GNP-based nanotheranostics [33,34]. An extensive body of literature has shown the effects of the surface chemistry and size of spherical GNPs on the protein corona, with the aim of controlling opsonization on GNPs [35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles

et al. 2021

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The heterogeneous distribution of delivery or treatment modalities within the tumor mass is a crucial limiting factor for a vast range of theranostic applications. Understanding the interactions between a nanomaterial and the tumor microenvironment will help to overcome challenges associated with tumor heterogeneity, as well as the clinical translation of nanotheranostic materials. This study aims to evaluate the influence of protein surface adsorption on gold nanoparticle (GNP) biodistribution using high-resolution computed tomography (CT) preclinical imaging in C57BL/6 mice harboring Lewis lung carcinoma (LLC) tumors. LLC provides a valuable model for study due to its highly heterogenous nature, which makes drug delivery to the tumor challenging. By controlling the adsorption of proteins on the GNP surface, we hypothesize that we can influence the intratumoral distribution pattern and particle retention. We performed an in vitro study to evaluate the uptake of GNPs by LLC cells and an in vivo study to assess and quantify the GNP biodistribution by injecting concentrated GNPs citrate-stabilized or passivated with bovine serum albumin (BSA) intratumorally into LLC solid tumors. Quantitative CT and inductively coupled plasma optical emission spectrometry (ICP-OES) results both confirm the presence of particles in the tumor 9 days post-injection (n = 8 mice/group). A significant difference is highlighted between citrate-GNP and BSA-GNP groups (** p < 0.005, Tukey’s multiple comparisons test), confirming that the protein corona of GNPs modifies intratumoral distribution and retention of the particles. In conclusion, our investigations show that the surface passivation of GNPs influences the mechanism of cellular uptake and intratumoral distribution in vivo, highlighting the spatial heterogeneity of the solid tumor.

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Section: Introductionmentioning

confidence: 99%

Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles

et al. 2021

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“…The effect of serum on NP penetration in the spheroid was also investigated in this experiment [ 46 ]. The images and distributions of various MSNs in the spheroid in the serum-containing medium are depicted in Figure 5 .…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Nanoparticle Penetration in the Tumor Spheroid Using Two-Photon Microscopy

Pratiwi

Peng

et al. 2020

Biomedicines

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Mesoporous silica nanoparticles (MSNs) have emerged as a prominent nanomedicine platform, especially for tumor-related nanocarrier systems. However, there is increasing concern about the ability of nanoparticles (NPs) to penetrate solid tumors, resulting in compromised antitumor efficacy. Because the physicochemical properties of NPs play a significant role in their penetration and accumulation in solid tumors, it is essential to systematically study their relationship in a model system. Here, we report a multihierarchical assessment of the accumulation and penetration of fluorescence-labeled MSNs with nine different physicochemical properties in tumor spheroids using two-photon microscopy. Our results indicated that individual physicochemical parameters separately could not define the MSNs’ ability to accumulate in a deeper tumor region; their features are entangled. We observed that the MSNs’ stability determined their success in reaching the hypoxia region. Moreover, the change in the MSNs’ penetration behavior postprotein crowning was associated with both the original properties of NPs and proteins on their surfaces.

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“…Certain components of the so-called protein corona such as Opsonins (IgG, complement and many others) may accelerate the RES uptake of coated nanoparticles [ 44 , 45 ]. On the other hand, there are so-called proteins dysopsonins, such as albumin, which, if coated with particles, prevent the activation of the complement, thus prolonging the circulation time and reducing toxicity [ 46 , 47 ]. The rate of adsorbtion and desorption for each protein determines the retention time in the corona and the way it interacts with the nanoparticle surface.…”

Section: The Composition Of Protein Coronamentioning

confidence: 99%

A review of the current understanding of nanoparticles protein corona composition

Breznica

Koliqi

Daka

2020

Medicine and Pharmacy Reports

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Upon entering into the biological environments, the surface of the nanoparticles is immediately coated with proteins and form the so-called a protein corona due to which a nanoparticle changes its "synthetic" identity to a new "biological" identity. Different types of nanoparticles have different protein binding profiles, which is why they have different protein corona composition and therefore it cannot be said that there is a universal protein corona. The composition and amount of protein in the corona depends on the physical and chemical characteristics of the nanoparticles, the type of biological medium and the exposure time. Protein corona increases the diameter but also changes the composition of the surface of the nanoparticles and these changes affect biodistribution, efficacy, and toxicity of the nanoparticles.

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Protein Corona-Enabled Systemic Delivery and Targeting of Nanoparticles

Cited by 49 publications

References 96 publications

Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles

Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles

Evaluation of Nanoparticle Penetration in the Tumor Spheroid Using Two-Photon Microscopy

A review of the current understanding of nanoparticles protein corona composition

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