Protein corona components of polyethylene glycol-conjugated organosilica nanoparticles modulates macrophage uptake

Kim, Hyungjin; Röth, Daniel; Isoe, Yasuhiro; Hayashi, Koichiro; Mochizuki, Chihiro; Kalkum, Markus; Nakamura, Michihiro

doi:10.1016/j.colsurfb.2020.111527

Cited by 28 publications

(25 citation statements)

References 56 publications

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“…Such a high incidence of low Au uptake may not be solely because more AuNRs@BSA 100 were monodisperse, but also because BSA pre-coating depressed the incidence of cellular recognition and uptake of AuNRs. Therefore, our single-cell quantification confirmed the stealth effect of BSA coating on NPs, consistent with previous reports [ 37 , 38 ]. But nevertheless, cellular uptake of AuNRs in vertically cultured cells was still quite heterogeneous.…”

Section: Discussionsupporting

confidence: 92%

Impact of Albumin Pre-Coating on Gold Nanoparticles Uptake at Single-Cell Level

Wang

et al. 2022

Nanomaterials

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Nanoparticles (NPs) suspension is thermodynamically unstable, agglomeration and sedimentation may occur after introducing NPs into a physiological solution, which in turn affects their recognition and uptake by cells. In this work, rod-like gold NPs (AuNRs) with uniform morphology and size were synthesized to study the impact of bovine serum albumin (BSA) pre-coating on the cellular uptake of AuNRs. A comparison study using horizontal and vertical cell culture configurations was performed to reveal the effect of NPs sedimentation on AuNRs uptake at the single-cell level. Our results demonstrate that the well-dispersed AuNRs-BSA complexes were more stable in culture medium than the pristine AuNRs, and therefore were less taken up by cells. The settled AuNRs agglomerates, although only a small fraction of the total AuNRs, weighed heavily in determining the average AuNRs uptake at the population level. These findings highlight the necessity of applying single-cell quantification analysis in the study of the mechanisms underlying the cellular uptake of NPs.

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Section: Discussionsupporting

confidence: 92%

Impact of Albumin Pre-Coating on Gold Nanoparticles Uptake at Single-Cell Level

Wang

et al. 2022

Nanomaterials

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“…This observation matches with previous NP studies showing an inverse correlation between the PEG chain length and particle uptake. 30 Higher concentrations of bare disks had little impact on uptake by RAW 264.7 and MutuDC1, whereas uptake of bare disks by HeLa cells was not observed even at a concentration of 1 nM, confirming differential uptake rates by different cell types. These results clearly show the importance of coating the NPs in cases where the target cells perform poor or slow uptake.…”

Section: Resultsmentioning

confidence: 82%

Strategic Insights into Engineering Parameters Affecting Cell Type-Specific Uptake of DNA-Based Nanomaterials

et al. 2022

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DNA-based nanomaterials are gaining popularity as uniform and programmable bioengineering tools as a result of recent solutions to their weak stability under biological conditions. The DNA nanotechnology platform uniquely allows decoupling of engineering parameters to comprehensively study the effect of each upon cellular encounter. We here present a systematic analysis of the effect of surface parameters of DNA-based nanoparticles on uptake in three different cell models: tumor cells, macrophages, and dendritic cells. The influence of surface charge, stabilizing coating, fluorophore types, functionalization technique, and particle concentration employed is found to cause significant differences in material uptake among these cell types. We therefore provide new insights into the large variance in cell type-specific uptake, highlighting the necessity of proper engineering and careful assay development when DNA-based materials are used as tools in bioengineering and as future nanotherapeutic agents.

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“…39 Since the biological identity could cause an unexpected response including the behaviour and distribution of NPs and cellular interaction like uptake, the effect of physiological factors on the identity of NPs needs to be considered in order to predict their cytotoxicity. 46,57–59…”

Section: Nano–bio Interactions In Vivo: Exploring the Behaviour Of Na...mentioning

confidence: 99%